| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Unidades Centrais. |
|
Data corrente: |
23/10/2018 |
|
Data da última atualização: |
23/10/2018 |
|
Autoria: |
MATTO, C.; VARELA, G.; BRAGA, V.; VICO, V.; GIANNEECHINI, R. E.; RIVERO, R. |
|
Afiliação: |
Carolina Matto; Gustavo Varela; Valeria Braga; Victoria Vico; Ruben E. Gianneechini; Rodolfo Rivero. |
|
Título: |
Detection of Listeria spp. in cattle and environment of pasture-based dairy farms. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 9, p. 1734-1741, setembro 2018 |
|
Idioma: |
Inglês |
|
Notas: |
Título em português: Detecção de Listeria spp. em gados leiteiros no meio ambiente com base na pastagem. |
|
Conteúdo: |
The aim of the study was to detect Listeria spp., particularly Listeria monocytogenes, in cattle and environment of pasture based dairy farms in Paysandú, Uruguay. A two-stage sampling was conducted, 10 farms were selected by probability proportional to size. A single visit was made to each farm. Samples from bovine faeces, feedstuffs, bulk tank milk, drinking water and soil from the entry and exit pens of the milking parlour were collected for bacteriological studies. PCR assays were used to confirm species and determine the serotype profile of L. monocytogenes isolates. AscI-pulsed-field gel electrophoresis was done to genetically compare them. Listeria spp. were isolated from eight of ten dairy farms, whereas L. monocytogenes in three of them. Serotype distribution in L. monocytogenes was as follows: 1/2a, three isolates; 4b, one isolate. L. monocytogenes or L. innocua excreted from clinically healthy milking cows was detected via faeces. In feedstuffs, only one L. monocytogenes 1/2a isolate from a pasture was obtained. The strain was identical by PFGE to an isolate 1/2a obtained from a pool of milking cow feces that grazed on this farm. No isolation of Listeria spp. was retrieved from the bulk tank milk or drinking water from any of the farms. Listeria innocua was detected in 13 feedstuffs and seven samples of soil from the entry and exit pens of the milking parlour. This is a first local study that confirms the presence of Listeria spp. including L. monocytogenes in healthy cattle and environment of pasture-based dairy farms. These results suggest the potential role that healthy cattle and their sub-products would play as a source of these agents for humans and/or others animals. More detailed studies that include genetic comparison of human and animal isolates are required in order to clearly establish the epidemiological relationship. MenosThe aim of the study was to detect Listeria spp., particularly Listeria monocytogenes, in cattle and environment of pasture based dairy farms in Paysandú, Uruguay. A two-stage sampling was conducted, 10 farms were selected by probability proportional to size. A single visit was made to each farm. Samples from bovine faeces, feedstuffs, bulk tank milk, drinking water and soil from the entry and exit pens of the milking parlour were collected for bacteriological studies. PCR assays were used to confirm species and determine the serotype profile of L. monocytogenes isolates. AscI-pulsed-field gel electrophoresis was done to genetically compare them. Listeria spp. were isolated from eight of ten dairy farms, whereas L. monocytogenes in three of them. Serotype distribution in L. monocytogenes was as follows: 1/2a, three isolates; 4b, one isolate. L. monocytogenes or L. innocua excreted from clinically healthy milking cows was detected via faeces. In feedstuffs, only one L. monocytogenes 1/2a isolate from a pasture was obtained. The strain was identical by PFGE to an isolate 1/2a obtained from a pool of milking cow feces that grazed on this farm. No isolation of Listeria spp. was retrieved from the bulk tank milk or drinking water from any of the farms. Listeria innocua was detected in 13 feedstuffs and seven samples of soil from the entry and exit pens of the milking parlour. This is a first local study that confirms the presence of Listeria spp. including L. monocytogenes in hea... Mostrar Tudo |
|
Palavras-Chave: |
Bacterioses; Dairy farms; Faecal shedding; Graze; Listeria spp. |
|
Thesagro: |
Bacteriose; Bovino; Fezes; Gado Leiteiro; Leite; Listeriose; Meio Ambiente; Pastagem. |
|
Thesaurus Nal: |
Cattle; Listeriosis; Milk. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/184888/1/Detection-of-Listeria-spp.-in-cattle.pdf
|
|
Marc: |
LEADER 02972naa a2200385 a 4500
001 2097965
005 2018-10-23
008 2018 bl uuuu u00u1 u #d
100 1 $aMATTO, C.
245 $aDetection of Listeria spp. in cattle and environment of pasture-based dairy farms.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Detecção de Listeria spp. em gados leiteiros no meio ambiente com base na pastagem.
520 $aThe aim of the study was to detect Listeria spp., particularly Listeria monocytogenes, in cattle and environment of pasture based dairy farms in Paysandú, Uruguay. A two-stage sampling was conducted, 10 farms were selected by probability proportional to size. A single visit was made to each farm. Samples from bovine faeces, feedstuffs, bulk tank milk, drinking water and soil from the entry and exit pens of the milking parlour were collected for bacteriological studies. PCR assays were used to confirm species and determine the serotype profile of L. monocytogenes isolates. AscI-pulsed-field gel electrophoresis was done to genetically compare them. Listeria spp. were isolated from eight of ten dairy farms, whereas L. monocytogenes in three of them. Serotype distribution in L. monocytogenes was as follows: 1/2a, three isolates; 4b, one isolate. L. monocytogenes or L. innocua excreted from clinically healthy milking cows was detected via faeces. In feedstuffs, only one L. monocytogenes 1/2a isolate from a pasture was obtained. The strain was identical by PFGE to an isolate 1/2a obtained from a pool of milking cow feces that grazed on this farm. No isolation of Listeria spp. was retrieved from the bulk tank milk or drinking water from any of the farms. Listeria innocua was detected in 13 feedstuffs and seven samples of soil from the entry and exit pens of the milking parlour. This is a first local study that confirms the presence of Listeria spp. including L. monocytogenes in healthy cattle and environment of pasture-based dairy farms. These results suggest the potential role that healthy cattle and their sub-products would play as a source of these agents for humans and/or others animals. More detailed studies that include genetic comparison of human and animal isolates are required in order to clearly establish the epidemiological relationship.
650 $aCattle
650 $aListeriosis
650 $aMilk
650 $aBacteriose
650 $aBovino
650 $aFezes
650 $aGado Leiteiro
650 $aLeite
650 $aListeriose
650 $aMeio Ambiente
650 $aPastagem
653 $aBacterioses
653 $aDairy farms
653 $aFaecal shedding
653 $aGraze
653 $aListeria spp
700 1 $aVARELA, G.
700 1 $aBRAGA, V.
700 1 $aVICO, V.
700 1 $aGIANNEECHINI, R. E.
700 1 $aRIVERO, R.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 9, p. 1734-1741, setembro 2018
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 500 | |
| 1. |  | MACHADO, R. Avaliação do sêmen congelado de ovinos. In: SEMINÁRIO DE PESQUISA AGROPECUÁRIA DO PIAUÍ, 8.; SIMPÓSIO AGROPECUÁRIO E FLORESTAL DO MEIO-NORTE, 1., 1994, Teresina. Pesquisa e desenvolvimento para o Meio-Norte: resumos. Teresina: EMBRAPA-CPAMN; São Luís: EMAPA, 1994. p. 81. (EMBRAPA-CPAMN. Documentos, 13).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
|    |
| 2. | | MACHADO, R. Avaliação do sêmen congelado de ovinos. In: SEMINÁRIO DE PESQUISA AGROPECUÁRIA DO PIAUÍ, 8., 1994, Teresina. Anais... Teresina: Embrapa-CPAMN, 1997. p. 249-252. (EMBRAPA-CPAMN. Documentos, 16).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 9. | | MACHADO, R. Diretrizes éticas para a experimentação animal na Embrapa. In: REUNIÃO ANUAL DA REGIÃO BRASILEIRA DA SOCIEDADE INTERNACIONAL DE BIOMETRIA, 57, 2012. Modelos da regressão: aplicações interdisciplinares - resumos. Piracicaba: Região Brasileira da Sociedade Internacional de Biometria, 2012. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| 15. | | MACHADO, R. Manejo reprodutivo em sistemas intensivos de produção leite. In: SEMANA DO ESTUDANTE, 12., 1998, Sao Carlos, SP. Estrategias para intensificacao da producao de leite : situacao atual e perspectivas: anais. São Carlos: Embrapa Pecuária Sudeste, 1998. p.80-97. Coordenado por Rogerio Taveira Barbosa, Andre Luiz Monteiro Novo, Pedro Franklin Barbosa, Sergio Novita Esteves.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| 19. | | MACHADO, R. Sincronização do estro. In: BARBOSA, R. T.; ESTEVES, S. N.; BARBOSA, P. F. (Ed.). Intensificação da bovinocultura de corte: estratégias de manejo reprodutivo e sanitário. São Carlos: EMBRAPA-CPPSE, 1997. p.19-28. p.19-28. (EMBRAPA-CPPSE. Documentos, 26).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| Registros recuperados : 500 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
