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Registro Completo |
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
22/03/2008 |
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Data da última atualização: |
09/11/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BARRETO, A. L. H.; VASCONCELOS, I. M.; GRANGEIRO, T. B.; MELO, V. M. M.; MATOS, T. E.; ELOY, Y. R. G.; FERNANDES, C. F.; TORRES, D. C.; FREIRE FILHO, F. R.; FREIRE, F. C. O.; OLIVEIRA, J. T. A. |
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Afiliação: |
ANA LUCIA HORTA BARRETO, CPAMN; ILKA M. VASCONCELOS; FRANCISCO RODRIGUES FREIRE FILHO, CPAMN. |
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Título: |
Infection process and host defense responses in compatible and incompatible interactions between Cowpea (Vigna unguiculata) and Colletotrichum gloeosporioides. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
International Journal of Plant Sciences, Chicago, v. 168, n. 2, p. 193-203, 2007. |
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ISSN: |
1058-5893 |
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Idioma: |
Inglês |
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Conteúdo: |
The infection process of Colletotrichum gloeosporioides was examined by light microscopy on two cowpea genotypes, BR 3 Tracuateua and TE 97-411. In the susceptible genotype BR 3 Tracuateua, the pathogen acted as an intracellular hemibiotroph. Invasion of cowpea primary leaves occurred preferentially through the leaf epidermal cells by penetration tubes that emerged from appressoria. The penetration tube swelled within 48 h to form a spherical infection vesicle inside the epidermal cell in a biotrophic interaction. Subsequently, it became multilobed and multiseptated. By 4 d after inoculation, one or more large primary hyphae emerged from the lateral lobes and grew intracellularly and further colonized several adjacent epidermal host cells, characterizing the necrotrophic phase of infection. Primary leaves of the resistant cowpea genotype, TE 97-411, showed enhanced penetration resistance to C. gloeosporioides associated with higher epidermal H2O2 accumulation beneath appressoria and primary germ tubes, papilla formation, and increased phenylalanine ammonia-lyase activity, possibly related to accumulation of phenolic compounds and host cell wall lignification. Macroscopic examination of the primary leaves revealed the presence of shrunken necrotic lesions characteristic of anthracnose in infected BR 3 Tracuateua, whereas in the genotype TE 97-411, cell death was also observed but only in a reduced percentage of the infection sites. In summary, the results obtained in this study suggested that TE 97-411 is the resistant genotype to C. gloeosporioides compared with BR 3 Tracuateua because it developed more effective defense responses against the establishment of the pathogen. MenosThe infection process of Colletotrichum gloeosporioides was examined by light microscopy on two cowpea genotypes, BR 3 Tracuateua and TE 97-411. In the susceptible genotype BR 3 Tracuateua, the pathogen acted as an intracellular hemibiotroph. Invasion of cowpea primary leaves occurred preferentially through the leaf epidermal cells by penetration tubes that emerged from appressoria. The penetration tube swelled within 48 h to form a spherical infection vesicle inside the epidermal cell in a biotrophic interaction. Subsequently, it became multilobed and multiseptated. By 4 d after inoculation, one or more large primary hyphae emerged from the lateral lobes and grew intracellularly and further colonized several adjacent epidermal host cells, characterizing the necrotrophic phase of infection. Primary leaves of the resistant cowpea genotype, TE 97-411, showed enhanced penetration resistance to C. gloeosporioides associated with higher epidermal H2O2 accumulation beneath appressoria and primary germ tubes, papilla formation, and increased phenylalanine ammonia-lyase activity, possibly related to accumulation of phenolic compounds and host cell wall lignification. Macroscopic examination of the primary leaves revealed the presence of shrunken necrotic lesions characteristic of anthracnose in infected BR 3 Tracuateua, whereas in the genotype TE 97-411, cell death was also observed but only in a reduced percentage of the infection sites. In summary, the results obtained in this stu... Mostrar Tudo |
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Palavras-Chave: |
Cowpea; Defense responses; Infection process. |
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Thesagro: |
Colletotrichum Gloeosporioides. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02659naa a2200301 a 4500
001 1069732
005 2023-11-09
008 2007 bl uuuu u00u1 u #d
022 $a1058-5893
100 1 $aBARRETO, A. L. H.
245 $aInfection process and host defense responses in compatible and incompatible interactions between Cowpea (Vigna unguiculata) and Colletotrichum gloeosporioides.
260 $c2007
520 $aThe infection process of Colletotrichum gloeosporioides was examined by light microscopy on two cowpea genotypes, BR 3 Tracuateua and TE 97-411. In the susceptible genotype BR 3 Tracuateua, the pathogen acted as an intracellular hemibiotroph. Invasion of cowpea primary leaves occurred preferentially through the leaf epidermal cells by penetration tubes that emerged from appressoria. The penetration tube swelled within 48 h to form a spherical infection vesicle inside the epidermal cell in a biotrophic interaction. Subsequently, it became multilobed and multiseptated. By 4 d after inoculation, one or more large primary hyphae emerged from the lateral lobes and grew intracellularly and further colonized several adjacent epidermal host cells, characterizing the necrotrophic phase of infection. Primary leaves of the resistant cowpea genotype, TE 97-411, showed enhanced penetration resistance to C. gloeosporioides associated with higher epidermal H2O2 accumulation beneath appressoria and primary germ tubes, papilla formation, and increased phenylalanine ammonia-lyase activity, possibly related to accumulation of phenolic compounds and host cell wall lignification. Macroscopic examination of the primary leaves revealed the presence of shrunken necrotic lesions characteristic of anthracnose in infected BR 3 Tracuateua, whereas in the genotype TE 97-411, cell death was also observed but only in a reduced percentage of the infection sites. In summary, the results obtained in this study suggested that TE 97-411 is the resistant genotype to C. gloeosporioides compared with BR 3 Tracuateua because it developed more effective defense responses against the establishment of the pathogen.
650 $aColletotrichum Gloeosporioides
653 $aCowpea
653 $aDefense responses
653 $aInfection process
700 1 $aVASCONCELOS, I. M.
700 1 $aGRANGEIRO, T. B.
700 1 $aMELO, V. M. M.
700 1 $aMATOS, T. E.
700 1 $aELOY, Y. R. G.
700 1 $aFERNANDES, C. F.
700 1 $aTORRES, D. C.
700 1 $aFREIRE FILHO, F. R.
700 1 $aFREIRE, F. C. O.
700 1 $aOLIVEIRA, J. T. A.
773 $tInternational Journal of Plant Sciences, Chicago$gv. 168, n. 2, p. 193-203, 2007.
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Embrapa Meio-Norte (CPAMN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Amazônia Ocidental. |
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Data corrente: |
28/08/2023 |
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Data da última atualização: |
06/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SOUSA, T. F.; REÇA, B. N. P. V.; CASTRO, G. S.; SILVA, I. J. S. da; CANIATO, F. F.; JÚNIOR, M. B. de A.; YAMAGISHI, M. E. B.; KOOLEN, H. H. F.; BATAGLION, G. A.; HANADA, R. E.; SILVA, G. F. da. |
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Afiliação: |
THIAGO FERNANDES SOUSA, UNIVERSIDADE FEDERAL DO AMAZONAS; BRUNA NAYARA PANTOJA VIEIRA REÇA, INSTITUTO NACIONAL DE PESQUISAS DA AMAZÔNIA; GLEUCINEI SANTOS CASTRO, UNIVERSIDADE DO ESTADO DO AMAZONAS; INGRIDE JARLINE SANTOS DA SILVA, UNIVERSIDADE FEDERAL DO AMAZONAS; FERNANDA FÁTIMA CANIATO, UNIVERSIDADE FEDERAL DO AMAZONAS; MOYSÉS BATISTA DE ARAÚJO JÚNIOR, UNIVERSIDADE FEDERAL DO AMAZONAS; MICHEL EDUARDO BELEZA YAMAGISHI, CNPTIA; HECTOR HENRIQUE FERREIRA KOOLEN, UNIVERSIDADE FEDERAL DO AMAZONAS; GIOVANA ANCESKI BATAGLION, UNIVERSIDADE FEDERAL DO AMAZONAS; ROGÉRIO EIJI HANADA, INSTITUTO NACIONAL DE PESQUISAS DA AMAZÔNIA; GILVAN FERREIRA DA SILVA, CPAA. |
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Título: |
Trichoderma agriamazonicum sp. nov. (Hypocreaceae), a new ally in the control of phytopathogens. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Microbiological Research, v. 275, 127469, Oct. 2023. |
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ISSN: |
1618-0623 |
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DOI: |
https://doi.org/10.1016/j.micres.2023.127469 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract. The genus Trichoderma comprises more than 500 valid species and is commonly used in agriculture for the control of plant diseases. In the present study, a Trichoderma species isolated from Scleronema micranthum (Malvaceae) has been extensively characterized and the morphological and phylogenetic data support the proposition of a new fungal species herein named Trichoderma agriamazonicum. This species inhibited the mycelial growth of all the nine phytopathogens tested both by mycoparasitism and by the production of VOCs, with a highlight for the inhibition of Corynespora cassiicola and Colletotrichum spp. The VOCs produced by T. agriamazonicum were able to control Capsicum chinense fruit rot caused by Colletotrichum scovillei and no symptoms were observed after seven days of phytopathogen inoculation. GC-MS revealed the production of mainly 6-amyl-?-pyrone, 1-octen-3-ol and 3-octanone during interaction with C. scovillei in C. chinense fruit. The HLPC-MS/MS analysis allowed us to annotate trikoningin KBII, hypocrenone C, 5-hydroxy-de-O-methyllasiodiplodin and unprecedented 7-mer peptaibols and lipopeptaibols. Comparative genomic analysis of five related Trichoderma species reveals a high number of proteins shared only with T. koningiopsis, mainly the enzymes related to oxidative stress. Regarding the CAZyme composition, T. agriamazonicum is most closely related to T. atroviride. A high protein copy number related to lignin and chitin degradation is observed for all Trichoderma spp. analyzed, while the presence of licheninase GH12 was observed only in T. agriamazonicum. Genome mining analysis identified 33 biosynthetic gene clusters (BGCs) of which 27 are new or uncharacterized, and the main BGCs are related to the production of polyketides. These results demonstrate the potential of this newly described species for agriculture and biotechnology. MenosAbstract. The genus Trichoderma comprises more than 500 valid species and is commonly used in agriculture for the control of plant diseases. In the present study, a Trichoderma species isolated from Scleronema micranthum (Malvaceae) has been extensively characterized and the morphological and phylogenetic data support the proposition of a new fungal species herein named Trichoderma agriamazonicum. This species inhibited the mycelial growth of all the nine phytopathogens tested both by mycoparasitism and by the production of VOCs, with a highlight for the inhibition of Corynespora cassiicola and Colletotrichum spp. The VOCs produced by T. agriamazonicum were able to control Capsicum chinense fruit rot caused by Colletotrichum scovillei and no symptoms were observed after seven days of phytopathogen inoculation. GC-MS revealed the production of mainly 6-amyl-?-pyrone, 1-octen-3-ol and 3-octanone during interaction with C. scovillei in C. chinense fruit. The HLPC-MS/MS analysis allowed us to annotate trikoningin KBII, hypocrenone C, 5-hydroxy-de-O-methyllasiodiplodin and unprecedented 7-mer peptaibols and lipopeptaibols. Comparative genomic analysis of five related Trichoderma species reveals a high number of proteins shared only with T. koningiopsis, mainly the enzymes related to oxidative stress. Regarding the CAZyme composition, T. agriamazonicum is most closely related to T. atroviride. A high protein copy number related to lignin and chitin degradation is observed for all ... Mostrar Tudo |
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Palavras-Chave: |
Amazon fungi; Biocontrol; Biocontrole; Fitopatógenos; Fungos amazônicos; Peptaibols; Peptídeos antibióticos; Phytopathogens; Post-harvest; Trichoderma agriamazonicum. |
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Thesagro: |
Agricultura; Pós-Colheita. |
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Thesaurus NAL: |
Agriculture; Hypocreaceae. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03140naa a2200433 a 4500
001 2156145
005 2023-09-06
008 2023 bl uuuu u00u1 u #d
022 $a1618-0623
024 7 $ahttps://doi.org/10.1016/j.micres.2023.127469$2DOI
100 1 $aSOUSA, T. F.
245 $aTrichoderma agriamazonicum sp. nov. (Hypocreaceae), a new ally in the control of phytopathogens.$h[electronic resource]
260 $c2023
520 $aAbstract. The genus Trichoderma comprises more than 500 valid species and is commonly used in agriculture for the control of plant diseases. In the present study, a Trichoderma species isolated from Scleronema micranthum (Malvaceae) has been extensively characterized and the morphological and phylogenetic data support the proposition of a new fungal species herein named Trichoderma agriamazonicum. This species inhibited the mycelial growth of all the nine phytopathogens tested both by mycoparasitism and by the production of VOCs, with a highlight for the inhibition of Corynespora cassiicola and Colletotrichum spp. The VOCs produced by T. agriamazonicum were able to control Capsicum chinense fruit rot caused by Colletotrichum scovillei and no symptoms were observed after seven days of phytopathogen inoculation. GC-MS revealed the production of mainly 6-amyl-?-pyrone, 1-octen-3-ol and 3-octanone during interaction with C. scovillei in C. chinense fruit. The HLPC-MS/MS analysis allowed us to annotate trikoningin KBII, hypocrenone C, 5-hydroxy-de-O-methyllasiodiplodin and unprecedented 7-mer peptaibols and lipopeptaibols. Comparative genomic analysis of five related Trichoderma species reveals a high number of proteins shared only with T. koningiopsis, mainly the enzymes related to oxidative stress. Regarding the CAZyme composition, T. agriamazonicum is most closely related to T. atroviride. A high protein copy number related to lignin and chitin degradation is observed for all Trichoderma spp. analyzed, while the presence of licheninase GH12 was observed only in T. agriamazonicum. Genome mining analysis identified 33 biosynthetic gene clusters (BGCs) of which 27 are new or uncharacterized, and the main BGCs are related to the production of polyketides. These results demonstrate the potential of this newly described species for agriculture and biotechnology.
650 $aAgriculture
650 $aHypocreaceae
650 $aAgricultura
650 $aPós-Colheita
653 $aAmazon fungi
653 $aBiocontrol
653 $aBiocontrole
653 $aFitopatógenos
653 $aFungos amazônicos
653 $aPeptaibols
653 $aPeptídeos antibióticos
653 $aPhytopathogens
653 $aPost-harvest
653 $aTrichoderma agriamazonicum
700 1 $aREÇA, B. N. P. V.
700 1 $aCASTRO, G. S.
700 1 $aSILVA, I. J. S. da
700 1 $aCANIATO, F. F.
700 1 $aJÚNIOR, M. B. de A.
700 1 $aYAMAGISHI, M. E. B.
700 1 $aKOOLEN, H. H. F.
700 1 $aBATAGLION, G. A.
700 1 $aHANADA, R. E.
700 1 $aSILVA, G. F. da
773 $tMicrobiological Research$gv. 275, 127469, Oct. 2023.
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