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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
07/05/2013 |
Data da última atualização: |
17/01/2024 |
Autoria: |
LIMA-VERDE, I. B.; ROSSETTO, R.; MATOS, M. H. T.; CELESTINO, J. J. H.; BUENO, J. B.; SILVA, C. M. G.; FAUSTINO, L. R.; MORORÓ, M. B. S.; ARAÚJO, V. T.; CAMPELLO, C. C.; FIGUEIREDO, J. R. |
Título: |
Androstenedione and follicle stimulating hormone involvement in the viability and development of goat preantral follicles in vitro. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Animal Reproduction, Belo Horizonte, v. 7, n. 2, p. 80-89, Apr./Jun. 2010. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: This study investigated the effects of androstenedione and follicle-stimulating hormone (FSH) on the viability and growth of caprine preantral follicles. Ovarian tissues were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) containing androstenedione (0, 1, 10, 50, or 100 ng/ml), FSH (50 ng/ml), or a combination of these two hormones. Cultured and noncultured control tissues were processed for histological and fluorescence analysis. In comparison with noncultured control, a significant reduction was noted in the percentage of normal follicles in all treatments after 1 and 7 days of culture (except treatment with 1 ng/ml of androstenedione for 1 day). As the culture period progressed from 1 to 7 days, treatments with 10 ng/ml of androstenedione + FSH or 50 ng/ml of androstenedione alone maintained the percentage of normal follicles. After 1 day, treatments with 10, 50, or 100 ng/ml of androstenedione + FSH, or with 50 ng/ml of androstenedione alone had more developing follicles than fresh control tissue. When comparing the culture periods, treatments with 1, 10 or 100 ng/ml of androstenedione alone, or FSH alone, or FSH with 1 ng/ml of androstenedione, showed an increase in the percentage of developing follicles. After 1 and 7 days, there were no differences in oocyte and follicular diameter among the treated samples and non-cultured control or MEM+ cultured tissue. Fluorescence analysis demonstrated that only fragments cultured in 50 or 100 ng/ml of androstenedione + FSH had viable preantral follicles similar to those observed in MEM+alone. In conclusion, androstenedione at 50 or 100 ng/ml, either associated with FSH or at 50 ng/ml alone, plays an important role in the maintenance of caprine preantral follicle viability and activation after only a short in vitro culture period. In addition, after 7 days MEM+alone was efficient in the maintenance of viability and in follicular activation, showing the importance of basic medium composition. MenosAbstract: This study investigated the effects of androstenedione and follicle-stimulating hormone (FSH) on the viability and growth of caprine preantral follicles. Ovarian tissues were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) containing androstenedione (0, 1, 10, 50, or 100 ng/ml), FSH (50 ng/ml), or a combination of these two hormones. Cultured and noncultured control tissues were processed for histological and fluorescence analysis. In comparison with noncultured control, a significant reduction was noted in the percentage of normal follicles in all treatments after 1 and 7 days of culture (except treatment with 1 ng/ml of androstenedione for 1 day). As the culture period progressed from 1 to 7 days, treatments with 10 ng/ml of androstenedione + FSH or 50 ng/ml of androstenedione alone maintained the percentage of normal follicles. After 1 day, treatments with 10, 50, or 100 ng/ml of androstenedione + FSH, or with 50 ng/ml of androstenedione alone had more developing follicles than fresh control tissue. When comparing the culture periods, treatments with 1, 10 or 100 ng/ml of androstenedione alone, or FSH alone, or FSH with 1 ng/ml of androstenedione, showed an increase in the percentage of developing follicles. After 1 and 7 days, there were no differences in oocyte and follicular diameter among the treated samples and non-cultured control or MEM+ cultured tissue. Fluorescence analysis demonstrated that only fragments cultured in 50 or 100 ng/ml of andr... Mostrar Tudo |
Palavras-Chave: |
Esteroidogenese; Foliculo pre-antral; FSH. |
Thesagro: |
Caprino; Cultura in vitro; Endocrinologia; Hormônio; Ovário; Reprodução animal. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03032naa a2200349 a 4500 001 1957239 005 2024-01-17 008 2010 bl uuuu u00u1 u #d 100 1 $aLIMA-VERDE, I. B. 245 $aAndrostenedione and follicle stimulating hormone involvement in the viability and development of goat preantral follicles in vitro.$h[electronic resource] 260 $c2010 520 $aAbstract: This study investigated the effects of androstenedione and follicle-stimulating hormone (FSH) on the viability and growth of caprine preantral follicles. Ovarian tissues were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) containing androstenedione (0, 1, 10, 50, or 100 ng/ml), FSH (50 ng/ml), or a combination of these two hormones. Cultured and noncultured control tissues were processed for histological and fluorescence analysis. In comparison with noncultured control, a significant reduction was noted in the percentage of normal follicles in all treatments after 1 and 7 days of culture (except treatment with 1 ng/ml of androstenedione for 1 day). As the culture period progressed from 1 to 7 days, treatments with 10 ng/ml of androstenedione + FSH or 50 ng/ml of androstenedione alone maintained the percentage of normal follicles. After 1 day, treatments with 10, 50, or 100 ng/ml of androstenedione + FSH, or with 50 ng/ml of androstenedione alone had more developing follicles than fresh control tissue. When comparing the culture periods, treatments with 1, 10 or 100 ng/ml of androstenedione alone, or FSH alone, or FSH with 1 ng/ml of androstenedione, showed an increase in the percentage of developing follicles. After 1 and 7 days, there were no differences in oocyte and follicular diameter among the treated samples and non-cultured control or MEM+ cultured tissue. Fluorescence analysis demonstrated that only fragments cultured in 50 or 100 ng/ml of androstenedione + FSH had viable preantral follicles similar to those observed in MEM+alone. In conclusion, androstenedione at 50 or 100 ng/ml, either associated with FSH or at 50 ng/ml alone, plays an important role in the maintenance of caprine preantral follicle viability and activation after only a short in vitro culture period. In addition, after 7 days MEM+alone was efficient in the maintenance of viability and in follicular activation, showing the importance of basic medium composition. 650 $aCaprino 650 $aCultura in vitro 650 $aEndocrinologia 650 $aHormônio 650 $aOvário 650 $aReprodução animal 653 $aEsteroidogenese 653 $aFoliculo pre-antral 653 $aFSH 700 1 $aROSSETTO, R. 700 1 $aMATOS, M. H. T. 700 1 $aCELESTINO, J. J. H. 700 1 $aBUENO, J. B. 700 1 $aSILVA, C. M. G. 700 1 $aFAUSTINO, L. R. 700 1 $aMORORÓ, M. B. S. 700 1 $aARAÚJO, V. T. 700 1 $aCAMPELLO, C. C. 700 1 $aFIGUEIREDO, J. R. 773 $tAnimal Reproduction, Belo Horizonte$gv. 7, n. 2, p. 80-89, Apr./Jun. 2010.
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1. | | RAMOS, F. N.; MORTARA, S. R.; MONALISA-FRANCISCO, N.; ELIAS, J. P. C.; MENINI NETO, L.; FREITAS, L.; KERSTEN, R. de A.; AMORIM, A. M.; MATOS, F. B. de; NUNES-FREITAS, A. F.; ALCANTARA, S.; ALEXANDRE, M. H. N.; ALMEIDA-SCABBIA, R. J. de; ALMEIDA, O. J. G. de; ALVES, F. E.; ALVES, R. M. de O.; ALVIM, F. S.; ANDRADE, A. C. S. de; ANDRADE, S. de; AONA, L. Y. S.; ARAUJO. A. C.; ARAÚJO, K. C. T. de; ARIATI, V.; ASSIS, J. C.; AZEVEDO, C. O. de; BARBOSA, B. F.; BARBOSA, D. E. F.; BARBOSA, F. dos R.; BARROS, F. de; BASILIO, G. A.; BATAGHIN, F. A.; BERED, F.; BIANCHI, J. S.; BLUM, C. T.; BOETLER, C. R.; BONNET, A.; BRANCALION, P. H. S.; BREIER, T. B.; BRION, C. de T.; BUZATTO, C. R.; CABRAL, A.; CADORIN, T. J.; CAGLIONI, E.; CANÊZ, L.; CARDOSO, P. H.; CARVALHO, F. S. de; CARVALHO, R. G.; CATHARINO, E. L. M.; CEBALLOS, S. J.; CEREZINI, M. T.; CÉSAR, R. G.; CESTARI, C.; CHAVES, C. J. N.; CITADINE-ZANETTE, V.; COELHO, L. F. M.; COFFANI-NUNES, J. V.; COLARES, R.; COLLETTA, G. D.; CORRÊA, N. de M.; COSTA, A. F. da; COSTA, G. M. da; COSTA, L. M. S.; COSTA, N. G. S.; COUTO, D. R.; CRISTOFOLINI, C.; CRUZ, A. C. R. da; DEL NERI, L. A.; DI PASQUO, M.; DIAS, A. dos S.; DIAS, L. do C. D.; DISLICH, R.; DUARTE, M. C.; FABRICANTE, J. R.; FARACHE, F. H. A.; FARIA, A. P. G. de; FAXINA, C.; FERREIRA, M. T. M.; FISCHER, E.; FONSECA, C. R.; FONTOURA, T.; FRANCISCO, T. M.; FURTADO, S. G.; GALETTI, M.; GALETTI, M.; GARBIN, M. L.; GASPER, A. L. de; GOETZE, M.; GOMES-DA-SILVA, J.; GONÇALVES, M. F. A.; GONZAGA, D. R.; SILVA, A. C. G. e; GUARALDO, A. de C.; GUARINO, E. de S. G.; GUISLON, A. V.; HUDSON, L. B.; JARDIM, J. G.; JUNGBLUTH, P.; KAESER, S. dos S.; KESSOUS, I. M.; KOCH, N. M.; KUNIYOSHI, Y. S.; LABIAK, P. H.; LAPATE, M. E.; SANTOS, A. C. L.; LEAL, R. L. B.; LEITE, F. S.; LEITMAN, P.; LIBONI, A. P.; LIEBSCH, D.; LINGNER, D. V.; LOMBARDI, J. A.; LUCAS, E.; LUZZI, J. dos R.; MAI, P.; MANIA, L. F.; MANTOVANI, W.; MARAGNI, A. G.; MARQUES, M. C. M.; MARQUEZ, G.; MARTINS, C.; MARTINS, L. do N.; MARTINS, P. L. S. S.; MAZZIERO, F. F. F.; MELO, C. de A.; MELO, M. M. F. de; MENDES, A. F.; MESACASA, L.; MORELLATO, L. P. C.; MORENO, V. de S.; MULLER, A.; MURAKAMI, M. M. da S.; CECCONELLO, E.; NARDY, C.; NERVO, M. H.; NEVES, B.; NOGUEIRA, M. G. C.; NONATO, F. R.; OLIVEIRA-FILHO, A. T. de; OLIVEIRA, C. P. L. de; OVERBECK, G. E.; MARCUSSO, G. M.; PACIENCIA, M. L. B.; PADILHA, P.; PADILHA, P. T.; PEREIRA, A. C. A.; PEREIRA, L. C.; PEREIRA, R. A. S.; PINCHEIRA-ULBRICH, J.; PIRES, J. S. R.; PIZO, M. A.; PÔRTO, K. C.; RATTIS, L.; REIS, J. R. de M.; REIS, S. G. dos; ROCHA-PESSÔA, T. C.; ROCHA, C. F. D.; ROCHA, F. S.; RODRIGUES, A. R. P.; RODRIGUES, R. R.; ROGALSKI, J. M.; ROSANELLI, R. L.; ROSSADO, A.; ROSSATTO, D. R.; ROTHER, D. C.; RUIZ-MIRANDA, C. R.; SAITER, F. Z.; SAMPAIO, M. B.; SANTANA, L. D.; SANTOS, J. S. dos; SARTORELLO, R.; SAZIMA, M.; SCHMITT, J. L.; SCHNEIDER, G.; SCHROEDER, B. G.; SEVEGNANI, L.; SILVA JÚNIOR, V. O.; SILVA, F. R. da; SILVA, M. J. da; SILVA, M. P. P.; SILVA, R. G.; SILVA, S. M.; SINGER, R. B.; SIQUEIRA, G.; SOARES, L. E.; SOUSA, H. C. de; SPIELMANN, A.; TONETTI, V. R.; TONIATO, M. T. Z.; ULGUIM, P. S. B.; VAN DEN BERG, C.; VAN DEN BERG, E.; VARASSIN, I. G.; SILVA, I. B. V. da; VIBRANS, A. C.; WAECHTER, J. L.; WEISSENBERG, E. W.; WINDISCH, P. G.; WOLOWSKI, M.; YAÑEZ, A.; YOSHIKAWA, V. N.; ZANDONÁ, L. R.; ZANELLA, C. M.; ZANIN, E. M.; ZAPPI, D. C.; ZIPPARRO, V. B.; ZORZANELLI, J. P. F.; RIBEIRO, M. C. Atlantic epiphytes: a data set of vascular and non-vascular epiphyte plants and lichens from the Atlantic Forest. Ecology, v. 100, n. 2, e02541, 2019. 59 p.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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