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Registro Completo |
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
15/02/2019 |
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Data da última atualização: |
15/02/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BANDEIRA, A. M. P.; MATOS, J. E.; MARIA, A. N.; CARNEIRO, P. C. F.; PURDY, P. H.; AZEVEDO, H. C. |
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Afiliação: |
ANDERSON MARQUES PINTO BANDEIRA, UFS; JOSE EDUARDO MATOS, UFS; ALEXANDRE NIZIO MARIA, CPATC; PAULO CESAR FALANGHE CARNEIRO, CPATC; PHILLIP H. PURDY, ARS; HYMERSON COSTA AZEVEDO, CPATC. |
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Título: |
The effects of gelatin supplementation prior to cooling on ram semen quality and fertility. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Animal Reproduction, v. 15, n. 1, p. 23-28, Jan./Mar. 2018. |
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DOI: |
10.21451/1984-3143-AR2017-0021 |
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Idioma: |
Inglês |
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Conteúdo: |
The physical and chemical characteristics of gelatin have been used to justify its inclusion in extenders to preserve the sperm quality and improve results of cervical artificial insemination with cooled semen. The objective of this study was to evaluate the effects of gelatin supplementation in cooling extender on the quality and fertility of ram semen stored at 5°C. Semen samples (n = 24) of Santa Inês rams (n = 6) were diluted in Glycine-Yolk-Milk extender without (control) or with 1.5% of gelatin. The samples were loaded into 0.25 mL straws, cooled to 5°C and stored vertically for 48 and 72 hours. Sample quality was evaluated using straw homogeneity tests based on pH, osmolality and the proportion of spermatozoa (PS) in both upper and lower segments of straws (US and LS), analyses of sperm motility, plasma and acrosomal membrane integrity, and by fertility after artificial insemination. Differences between the US and LS of straws were found for pH and PS (%). They were significant only in the control group at both times: pH ? 5.96 vs. 5.71 at 48 h and 6.13 vs. 5.89 at 72 h; PS ? 21.66 vs. 78.34 at 48 h and 20.87 vs. 79.13 at 72 h. Storage in gelatin had very little, to no effect on the sperm kinetics or on the sperm membrane integrity evaluations. The addition of gelatin to the extender did not affect the pregnancy rate which ranged from 4.4 to 26.1%. We conclude that gelatin is effective in maintaining the physical and chemical homogeneity of the semen samples. Further research is needed in order to optimize the use of gelatin supplementation and elucidate any potential benefits. MenosThe physical and chemical characteristics of gelatin have been used to justify its inclusion in extenders to preserve the sperm quality and improve results of cervical artificial insemination with cooled semen. The objective of this study was to evaluate the effects of gelatin supplementation in cooling extender on the quality and fertility of ram semen stored at 5°C. Semen samples (n = 24) of Santa Inês rams (n = 6) were diluted in Glycine-Yolk-Milk extender without (control) or with 1.5% of gelatin. The samples were loaded into 0.25 mL straws, cooled to 5°C and stored vertically for 48 and 72 hours. Sample quality was evaluated using straw homogeneity tests based on pH, osmolality and the proportion of spermatozoa (PS) in both upper and lower segments of straws (US and LS), analyses of sperm motility, plasma and acrosomal membrane integrity, and by fertility after artificial insemination. Differences between the US and LS of straws were found for pH and PS (%). They were significant only in the control group at both times: pH ? 5.96 vs. 5.71 at 48 h and 6.13 vs. 5.89 at 72 h; PS ? 21.66 vs. 78.34 at 48 h and 20.87 vs. 79.13 at 72 h. Storage in gelatin had very little, to no effect on the sperm kinetics or on the sperm membrane integrity evaluations. The addition of gelatin to the extender did not affect the pregnancy rate which ranged from 4.4 to 26.1%. We conclude that gelatin is effective in maintaining the physical and chemical homogeneity of the semen samples. Further ... Mostrar Tudo |
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Thesagro: |
Fertilidade Animal; Sêmen. |
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Thesaurus Nal: |
Animal fertility. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/192876/1/The-effects-of-gelatin-supplementation-p23-28-AR-2017-0021.pdf
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Marc: |
LEADER 02312naa a2200229 a 4500
001 2106085
005 2019-02-15
008 2018 bl uuuu u00u1 u #d
024 7 $a10.21451/1984-3143-AR2017-0021$2DOI
100 1 $aBANDEIRA, A. M. P.
245 $aThe effects of gelatin supplementation prior to cooling on ram semen quality and fertility.$h[electronic resource]
260 $c2018
520 $aThe physical and chemical characteristics of gelatin have been used to justify its inclusion in extenders to preserve the sperm quality and improve results of cervical artificial insemination with cooled semen. The objective of this study was to evaluate the effects of gelatin supplementation in cooling extender on the quality and fertility of ram semen stored at 5°C. Semen samples (n = 24) of Santa Inês rams (n = 6) were diluted in Glycine-Yolk-Milk extender without (control) or with 1.5% of gelatin. The samples were loaded into 0.25 mL straws, cooled to 5°C and stored vertically for 48 and 72 hours. Sample quality was evaluated using straw homogeneity tests based on pH, osmolality and the proportion of spermatozoa (PS) in both upper and lower segments of straws (US and LS), analyses of sperm motility, plasma and acrosomal membrane integrity, and by fertility after artificial insemination. Differences between the US and LS of straws were found for pH and PS (%). They were significant only in the control group at both times: pH ? 5.96 vs. 5.71 at 48 h and 6.13 vs. 5.89 at 72 h; PS ? 21.66 vs. 78.34 at 48 h and 20.87 vs. 79.13 at 72 h. Storage in gelatin had very little, to no effect on the sperm kinetics or on the sperm membrane integrity evaluations. The addition of gelatin to the extender did not affect the pregnancy rate which ranged from 4.4 to 26.1%. We conclude that gelatin is effective in maintaining the physical and chemical homogeneity of the semen samples. Further research is needed in order to optimize the use of gelatin supplementation and elucidate any potential benefits.
650 $aAnimal fertility
650 $aFertilidade Animal
650 $aSêmen
700 1 $aMATOS, J. E.
700 1 $aMARIA, A. N.
700 1 $aCARNEIRO, P. C. F.
700 1 $aPURDY, P. H.
700 1 $aAZEVEDO, H. C.
773 $tAnimal Reproduction$gv. 15, n. 1, p. 23-28, Jan./Mar. 2018.
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Registro original: |
Embrapa Tabuleiros Costeiros (CPATC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amapá. |
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Data corrente: |
04/11/2015 |
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Data da última atualização: |
14/03/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
OLIVEIRA, A. T. de; SANTOS, M. Q. de C.; LEMOS, J. R. G.; TAVARES-DIAS, M.; MARCON, J. L. |
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Afiliação: |
ADRIANO TEIXEIRA DE OLIVEIRA, IFAM, Campus Presidente Figueiredo, Manaus, AM.; MÁRCIO QUARA DE CARVALHO SANTOS, IFAM, Campus Coari, Manaus, AM.; JEFFERSON RAPHAEL GONZAGA LEMOS, FAMETRO, Manaus, AM.; MARCOS TAVARES-DIAS, CPAF-AP; JAYDIONE LUIZ MARCON, UFAM, Manaus, AM. |
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Título: |
Comparison of the effects of anticoagulants used in blood collection to determine blood parameters of free-living stingrays from the Potamotrygon genus (Elasmobranchii: Potamotrygonidae). |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Biota Amazônia, Macapá, v. 5, n. 3, p. 55-58, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
This study assessed the effectiveness of three anticoagulants in blood parameters of "cururu" stingrays Potamotrygon cf . histrix. Blood from ten individuals were collected and diluted with anticoagulants EDTA 5% and 10%, heparin 2500 and 5000 UI and sodium citrate 3.2%. A blood sample without anticoagulant was also evaluated. The blood of samples without anticoagulant and with sodium citrate 3.2% coagulated in 20% and 30% of the cases, respectively. Clumps of cells were observed during erythrocyte counting in 30% of samples with EDTA 10%, 40% of samples with heparin 2500 IU and 60% of samples with heparin 5000 IU. No alterations were observed on the erythrogram of "cururu" stingrays with different anticoagulants, the values of plasma glucose were similar in all groups and total protein levels were lower in the samples with EDTA 5% and 10%. The use of sodium citrate 3.2% is not recommended for blood sample conservation of Potamotrygon cf . histrix. stingrays, but anticoagulants did not affect the parameters analyzed in the determination of plasma glucose and erythrogram. Therefore , these results indicate that in order to blood coagulation the samples should be collected directly with any of these anticoagulants concentrations. |
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Palavras-Chave: |
Anticoagulante. |
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Thesagro: |
Hematologia; Sangue. |
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Thesaurus NAL: |
blood; hematology. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/132479/1/CPAF-AP-2015-Comparison-of-the-effects.pdf
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Marc: |
LEADER 02001naa a2200229 a 4500
001 2027937
005 2017-03-14
008 2015 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, A. T. de
245 $aComparison of the effects of anticoagulants used in blood collection to determine blood parameters of free-living stingrays from the Potamotrygon genus (Elasmobranchii$bPotamotrygonidae).$h[electronic resource]
260 $c2015
520 $aThis study assessed the effectiveness of three anticoagulants in blood parameters of "cururu" stingrays Potamotrygon cf . histrix. Blood from ten individuals were collected and diluted with anticoagulants EDTA 5% and 10%, heparin 2500 and 5000 UI and sodium citrate 3.2%. A blood sample without anticoagulant was also evaluated. The blood of samples without anticoagulant and with sodium citrate 3.2% coagulated in 20% and 30% of the cases, respectively. Clumps of cells were observed during erythrocyte counting in 30% of samples with EDTA 10%, 40% of samples with heparin 2500 IU and 60% of samples with heparin 5000 IU. No alterations were observed on the erythrogram of "cururu" stingrays with different anticoagulants, the values of plasma glucose were similar in all groups and total protein levels were lower in the samples with EDTA 5% and 10%. The use of sodium citrate 3.2% is not recommended for blood sample conservation of Potamotrygon cf . histrix. stingrays, but anticoagulants did not affect the parameters analyzed in the determination of plasma glucose and erythrogram. Therefore , these results indicate that in order to blood coagulation the samples should be collected directly with any of these anticoagulants concentrations.
650 $ablood
650 $ahematology
650 $aHematologia
650 $aSangue
653 $aAnticoagulante
700 1 $aSANTOS, M. Q. de C.
700 1 $aLEMOS, J. R. G.
700 1 $aTAVARES-DIAS, M.
700 1 $aMARCON, J. L.
773 $tBiota Amazônia, Macapá$gv. 5, n. 3, p. 55-58, 2015.
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Embrapa Amapá (CPAF-AP) |
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