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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
20/07/2021 |
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Data da última atualização: |
20/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MASCARIN, G. M.; IWANICKI, N. S.; RAMIREZ, J. L.; DELALIBERA JUNIOR, I.; DUNLAP, C. A. |
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Afiliação: |
GABRIEL MOURA MASCARIN, CNPMA; NATASHA SANT´ANNA IWANICKI, ESALQ-USP; JOSE LUIS RAMIREZ, USDA-ARS; ÍTALO DELALIBERA JUNIOR, ESALQ-USP. |
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Título: |
Transcriptional responses of Beauveria bassiana blastospores cultured under varying glucose concentrations. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Frontiers in Cellular and Infection Microbiology, v. 11, article 644372, 2021. |
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Páginas: |
p. 1-18. |
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ISSN: |
2235-2988 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Culturing the entomopathogenic fungus, Beauveria bassiana, under high glucose concentrations coupled with high aeration results in a fungal developmental shift from hyphal growth to mostly blastospores (yeast-like cells). The underlying molecular mechanisms involved in this shift remain elusive. A systematic transcriptome analysis of the differential gene expression was preformed to uncover the fungal transcriptomic response to osmotic and oxidative stresses associated with the resulting high blastospore yield. Differential gene expression was compared under moderate (10% w/v) and high (20% w/v) glucose concentrations daily for three days. The RNAseq-based transcriptomic results depicted a higher proportion of downregulated genes when the fungus was grown under 20% glucose than 10%. Additional experiments explored a broader glucose range (4, 8, 12, 16, 20% w/v) with phenotype assessment and qRT-PCR transcript abundance measurements of selected genes. Antioxidant, calcium transport, conidiation, and osmosensor-related genes were highly upregulated in higher glucose titers (16-20%) compared to growth in lower glucose (4-6%) concentrations. The class 1 hydrophobin gene (Hyd1) was highly expressed throughout the culturing. Hyd1 is known to be involved in spore coat rodlet layer assembly, and indicates that blastospores or another cell type containing hydrophobin 1 is expressed in the haemocoel during the infection process. Furthermore, we found implications of the HOG signaling pathway with upregulation of homologous genes Ssk2 and Hog1 for all fermentation time points under hyperosmotic medium (20% glucose). These findings expand our knowledge of the molecular mechanisms behind blastospore development and may help facilitate large-scale industrial production of B. bassiana blastospores for pest control applications. MenosAbstract: Culturing the entomopathogenic fungus, Beauveria bassiana, under high glucose concentrations coupled with high aeration results in a fungal developmental shift from hyphal growth to mostly blastospores (yeast-like cells). The underlying molecular mechanisms involved in this shift remain elusive. A systematic transcriptome analysis of the differential gene expression was preformed to uncover the fungal transcriptomic response to osmotic and oxidative stresses associated with the resulting high blastospore yield. Differential gene expression was compared under moderate (10% w/v) and high (20% w/v) glucose concentrations daily for three days. The RNAseq-based transcriptomic results depicted a higher proportion of downregulated genes when the fungus was grown under 20% glucose than 10%. Additional experiments explored a broader glucose range (4, 8, 12, 16, 20% w/v) with phenotype assessment and qRT-PCR transcript abundance measurements of selected genes. Antioxidant, calcium transport, conidiation, and osmosensor-related genes were highly upregulated in higher glucose titers (16-20%) compared to growth in lower glucose (4-6%) concentrations. The class 1 hydrophobin gene (Hyd1) was highly expressed throughout the culturing. Hyd1 is known to be involved in spore coat rodlet layer assembly, and indicates that blastospores or another cell type containing hydrophobin 1 is expressed in the haemocoel during the infection process. Furthermore, we found implications of the HOG ... Mostrar Tudo |
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Palavras-Chave: |
Biocontrol; Dimorphic growth; Liquid fermentation. |
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Thesagro: |
Beauveria Bassiana; Controle Biológico; Fermentação; Fungo Entomógeno; Fungo Para Controle Biológico. |
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Thesaurus Nal: |
Biological control; Cordycipitaceae; Liquid state fermentation; morphogenesis. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/224558/1/Mascarin-Transcriptional-responses-2021.pdf
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Marc: |
LEADER 02887naa a2200337 a 4500
001 2133044
005 2021-07-20
008 2021 bl uuuu u00u1 u #d
022 $a2235-2988
100 1 $aMASCARIN, G. M.
245 $aTranscriptional responses of Beauveria bassiana blastospores cultured under varying glucose concentrations.$h[electronic resource]
260 $c2021
300 $ap. 1-18.
520 $aAbstract: Culturing the entomopathogenic fungus, Beauveria bassiana, under high glucose concentrations coupled with high aeration results in a fungal developmental shift from hyphal growth to mostly blastospores (yeast-like cells). The underlying molecular mechanisms involved in this shift remain elusive. A systematic transcriptome analysis of the differential gene expression was preformed to uncover the fungal transcriptomic response to osmotic and oxidative stresses associated with the resulting high blastospore yield. Differential gene expression was compared under moderate (10% w/v) and high (20% w/v) glucose concentrations daily for three days. The RNAseq-based transcriptomic results depicted a higher proportion of downregulated genes when the fungus was grown under 20% glucose than 10%. Additional experiments explored a broader glucose range (4, 8, 12, 16, 20% w/v) with phenotype assessment and qRT-PCR transcript abundance measurements of selected genes. Antioxidant, calcium transport, conidiation, and osmosensor-related genes were highly upregulated in higher glucose titers (16-20%) compared to growth in lower glucose (4-6%) concentrations. The class 1 hydrophobin gene (Hyd1) was highly expressed throughout the culturing. Hyd1 is known to be involved in spore coat rodlet layer assembly, and indicates that blastospores or another cell type containing hydrophobin 1 is expressed in the haemocoel during the infection process. Furthermore, we found implications of the HOG signaling pathway with upregulation of homologous genes Ssk2 and Hog1 for all fermentation time points under hyperosmotic medium (20% glucose). These findings expand our knowledge of the molecular mechanisms behind blastospore development and may help facilitate large-scale industrial production of B. bassiana blastospores for pest control applications.
650 $aBiological control
650 $aCordycipitaceae
650 $aLiquid state fermentation
650 $amorphogenesis
650 $aBeauveria Bassiana
650 $aControle Biológico
650 $aFermentação
650 $aFungo Entomógeno
650 $aFungo Para Controle Biológico
653 $aBiocontrol
653 $aDimorphic growth
653 $aLiquid fermentation
700 1 $aIWANICKI, N. S.
700 1 $aRAMIREZ, J. L.
700 1 $aDELALIBERA JUNIOR, I.
700 1 $aDUNLAP, C. A.
773 $tFrontiers in Cellular and Infection Microbiology$gv. 11, article 644372, 2021.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 139 | |
| 66. |  | TOFANGSAZI, N.; BUSS, E. A.; MEAGHER, R.; MASCARIN, G. M.; ARTHURS, S. P. Thermal requirements and development of Herpetogramma phaeopteralis (Lepidoptera: Crambidae: Spilomelinae). Journal of Economic Entomology, Lanham, v. 105, n. 5, p. 1573-1580, Oct. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 68. | | PACHECO, K. R.; QUINTELA, E. D.; MASCARIN, G. M.; BOAVENTURA, H. A.; CORRÊA, L. F.; ROCHA, D. V. M. Aumento da germinação dos conídios de Metarhizium anisopliae na cutícula de Tibraca limbativentris por tiametoxam. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 26.; CONGRESSO LATINO-AMERICANO DE ENTOMOLOGIA, 9., 2016, Maceió. Anais... Brasília, DF : Embrapa, 2016. p. 274.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 70. | | SANTOS, T. T. M. dos; QUINTELA, E. D.; MASCARIN, G. M.; SANTANA, M. V.; SILVA, J. F. A. e. Compatibilidade entre o fungo entomopatogênico Isaria javanica e inseticidas sintéticos. In: SEMINÁRIO JOVENS TALENTOS, 10., 2016, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2016. p. 45. (Embrapa Arroz e Feijão. Documentos, 311).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 71. | | SILVA, R. A. da; QUINTELA, E. D.; MASCARIN, G. M.; BARRIGOSSI, J. A. F.; LIÃO, L. M. Compatibility of conventional agrochemicals used in rice crops with the entomopathogenic fungus Metarhizium anisopliae. Scientia Agricola, Piracicaba, v. 70, n. 3, p. 152-160, maio/jun. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 72. | | MORALES-REYES, C.; MASCARIN, G. M.; JACKSON, M. A.; HALL, D.; SANCHEZ-PENA, S. R.; ARTHURS, S. P. Comparison of aerial conidia and blastospores from two entomopathogenic fungi against Diaphorina citri (Hemiptera: Liviidae) under laboratory and greenhouse conditions. Biocontrol Science and Technology, v. 28, n. 8, p. 737-749, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 73. | | HUARTE-BONNET, C.; PAIXÃO, F. R. S. da; MASCARIN, G. M.; SANTANA, M.; FERNANDES, E. K. K.; PEDRINI, N. The entomopathogenic fungus Beauveria bassiana produces microsclerotia-like pellets mediated by oxidative stress and peroxisome biogenesis. Environmental Microbiology Reports, v. 11, n. 4, p. 518?524, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 76. | | MARTINEZ, J. M.; RODRIGUES, J.; MARRETO, R. N.; MASCARIN, G. M.; FERNANDES, E. K. K.; HUMBER, R. A.; LUZ, C. Efficacy of focal applications of a mycoinsecticide to control Aedes aegypti in Central Brazil. Applied Microbiology and Biotechnology, v. 105, n. 23, p. 8702-8714, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 77. | | MASCARIN, G. M.; DUNLAP, C. A.; BARRIGOSSI, J. A. F.; QUINTELA, E. D.; NORONHA JUNIOR, N. C. de. First record of epizootics in the ocola skipper, Panoquina ocola (Lepidopera: Hesperiidae), caused by Isaria tenuipes in flooded rice fields of Central Brazil. Journal of Applied Microbiology, v. 122, n. 4, p. 1020-1028, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 80. | | ARISTIZÁBAL, L. F.; SHRINER, S.; HOLLINGSWORTH, R.; MASCARIN, G. M.; CHAVES, B.; MATSUMOTO, T.; ARTHURS, S. P. Field sampling strategies for coffee berry borer (Coleoptera: Curculionidae: Scolytinae) infesting berries in coffee farms in Hawaii. International Journal of Tropical Insect Science, v. 38, n. 4, p. 418?426, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 139 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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