Data/hora: 23/09/2024 18:57:59

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	07/10/2021	
Data da última atualização: 	07/10/2021	
Autoria: 	OLIVEIRA, T. C. de A.; BRITO, M. A. P. P.; MARVAL, M. G. de; VICENTINI, N. M.; LANGE, C. C.	
Afiliação: 	THAIS CRISTINA DE ASSIS OLIVEIRA, Estudante, Universidade Federal de Juiz de Fora; MARIA APARECIDA VASCONCELOS PAIVA BRITO; MARCIA GIAMBIAGI DE MARVAL, Universidade Federal do Rio de Janeiro; NIVEA MARIA VICENTINI, CNPGL; CARLA CHRISTINE LANGE, CNPGL.	
Título: 	Identification of bovine mastitis pathogens using MALDI-TOF mass spectrometry in Brazil.	
Ano de publicação: 	2021	
Fonte/Imprenta: 	Journal of Dairy Research, v. 88, p. 302-306, 2021.	
DOI: 	https://doi.org/10.1017/ S0022029921000595	
Idioma: 	Inglês	
Conteúdo: 	In this Research Communication we evaluate the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify 380 bacteria isolated from cases of bovine mastitis in Brazil. MALDI-TOF MS identifications were compared to previous identifications by biochemical tests and 16S rRNA sequencing. MALDI-TOF MS achieved a typeability of 95.5%. The accuracy of MALDI-TOF MS for the identification of Staphylococcus isolates was 93.2%. The agreement between MALDI-TOF MS and biochemical identification of Streptococcus agalactiae was 96%, however, the agreement between these techniques for identifying other catalase-negative, Gram-positive cocci was lower. Agreement in identifying Gram-negative bacteria at the genus level was 90.5%. Our findings corroborate that MALDI-TOF MS is an accurate, rapid and simple technique for identifying bovine mastitis pathogens. The availability of this methodology in some research institutions would represent a significant step toward increasing the diagnosis and epidemiological studies of bovine mastitis and other animal infectious diseases in Brazil.	
Palavras-Chave: 	Mastite; Staphylococci; Streptococci.	
Thesagro: 	Bovino; Espectrometria; Estafilococo; Estreptococo; Infecção.	
Thesaurus Nal: 	Bovine mastitis; Mass spectrometry.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (25431-1) - UPC - AP - DD -  -  -  - DIS