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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
17/11/1999 |
Data da última atualização: |
23/08/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CANCADO, G. M. A.; LOGUERCIO, L. L.; MARTINS, P. R.; PARENTONI, S. N.; PAIVA, E.; BOREM, A.; LOPES, M. A. |
Afiliação: |
EMBRAPA/CNPMS; SIDNEY NETTO PARENTONI, CNPMS. |
Título: |
Hematoxylin staining as a phenotypic index for aluminum tolerance selection in tropical maize (Zea mays L.). |
Ano de publicação: |
1999 |
Fonte/Imprenta: |
Theoretical and Applied Genetics, New York, v. 99, n. 5, p. 747-754, 1999. |
Idioma: |
Inglês |
Conteúdo: |
Hematoxylin staining is an early indicator of aluminum (Al) toxicity effects on the apices of young, developing roots grown in nutrient solution. In this work, the potential of this technique as a reliable and reproducible phenotypic index for Al tolerance in tropical maize genotypes was assessed, with its performance systematically compared to two other parameters widely used in breeding programs - relative seminal-roots length (RSRL) and net seminal-root length (NSRL). Seeding roots from contrasting genotypes for Al sensitivity stained remarkably different after 24- and 48-h and 7-day exposures to 222 uM Al in nutrient solution, with the Al-dye complex being detected in both the outer (epidermis) and inner (cortex) portions of the roots from the sensitive cultivar. Hemotoxylin staining was compared to the RSRL and NSRL parameters using 20 families from the third generation of selfing (S3) following the cross between two contrasting inbred lines that had been previously classified by the RSRL index in an independent procedure. The coloration technique showed the highest capacity to discriminate among tolerant and sensitive genotypes and displayed significant correlation coefficient to the other two indexes. Evaluation of the results from diallel crosses involving nine inbred lines proved that hematoxylin staining was also particularly adequate for identifying expressive hybrid vigor, as demonstrated by the general (GCA) and specific (SCA) combining ability estimates obtained by using the three indexex simultaneously. Hence, hematoxylin staining of Al-stressed root apices appears to be a powerful tool to assist in Al-tolerance selection in tropical maize breeding programs. MenosHematoxylin staining is an early indicator of aluminum (Al) toxicity effects on the apices of young, developing roots grown in nutrient solution. In this work, the potential of this technique as a reliable and reproducible phenotypic index for Al tolerance in tropical maize genotypes was assessed, with its performance systematically compared to two other parameters widely used in breeding programs - relative seminal-roots length (RSRL) and net seminal-root length (NSRL). Seeding roots from contrasting genotypes for Al sensitivity stained remarkably different after 24- and 48-h and 7-day exposures to 222 uM Al in nutrient solution, with the Al-dye complex being detected in both the outer (epidermis) and inner (cortex) portions of the roots from the sensitive cultivar. Hemotoxylin staining was compared to the RSRL and NSRL parameters using 20 families from the third generation of selfing (S3) following the cross between two contrasting inbred lines that had been previously classified by the RSRL index in an independent procedure. The coloration technique showed the highest capacity to discriminate among tolerant and sensitive genotypes and displayed significant correlation coefficient to the other two indexes. Evaluation of the results from diallel crosses involving nine inbred lines proved that hematoxylin staining was also particularly adequate for identifying expressive hybrid vigor, as demonstrated by the general (GCA) and specific (SCA) combining ability estimates obtaine... Mostrar Tudo |
Thesagro: |
Aluminio. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/40394/1/Hematoxylin-staining.pdf
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Marc: |
LEADER 02337naa a2200205 a 4500 001 1482859 005 2020-08-23 008 1999 bl uuuu u00u1 u #d 100 1 $aCANCADO, G. M. A. 245 $aHematoxylin staining as a phenotypic index for aluminum tolerance selection in tropical maize (Zea mays L.).$h[electronic resource] 260 $c1999 520 $aHematoxylin staining is an early indicator of aluminum (Al) toxicity effects on the apices of young, developing roots grown in nutrient solution. In this work, the potential of this technique as a reliable and reproducible phenotypic index for Al tolerance in tropical maize genotypes was assessed, with its performance systematically compared to two other parameters widely used in breeding programs - relative seminal-roots length (RSRL) and net seminal-root length (NSRL). Seeding roots from contrasting genotypes for Al sensitivity stained remarkably different after 24- and 48-h and 7-day exposures to 222 uM Al in nutrient solution, with the Al-dye complex being detected in both the outer (epidermis) and inner (cortex) portions of the roots from the sensitive cultivar. Hemotoxylin staining was compared to the RSRL and NSRL parameters using 20 families from the third generation of selfing (S3) following the cross between two contrasting inbred lines that had been previously classified by the RSRL index in an independent procedure. The coloration technique showed the highest capacity to discriminate among tolerant and sensitive genotypes and displayed significant correlation coefficient to the other two indexes. Evaluation of the results from diallel crosses involving nine inbred lines proved that hematoxylin staining was also particularly adequate for identifying expressive hybrid vigor, as demonstrated by the general (GCA) and specific (SCA) combining ability estimates obtained by using the three indexex simultaneously. Hence, hematoxylin staining of Al-stressed root apices appears to be a powerful tool to assist in Al-tolerance selection in tropical maize breeding programs. 650 $aAluminio 700 1 $aLOGUERCIO, L. L. 700 1 $aMARTINS, P. R. 700 1 $aPARENTONI, S. N. 700 1 $aPAIVA, E. 700 1 $aBOREM, A. 700 1 $aLOPES, M. A. 773 $tTheoretical and Applied Genetics, New York$gv. 99, n. 5, p. 747-754, 1999.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
15/10/2021 |
Data da última atualização: |
15/10/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 3 |
Autoria: |
SILVA, P. C.; RIBEIRO JUNIOR, W. Q.; RAMOS, M. L. G.; CELESTINO, S. M. C.; SILVA, A. N.; CASARI, R. A. C. N.; SANTANA, C. C.; LIMA, C. A. de; WILLIAMS, T. C. R.; VINSON, C. C. |
Afiliação: |
PATRÍCIA CARVALHO DA SILVA; WALTER QUADROS RIBEIRO JUNIOR, CPAC; MARIA LUCRECIA GEROSA RAMOS; SONIA MARIA COSTA CELESTINO, CPAC; ALBERTO DO NASCIMENTO SILVA; RAPHAEL AUGUSTO DAS CHAGAS NOQUELI CASARI; CHARLES CARDOSO SANTANA; CRISTIANE ANDREA DE LIMA; THOMAS CHRISTOPHER RHYS WILLIAMS; CHRISTINA CLEO VINSON. |
Título: |
Quinoa for the Brazilian Cerrado: Agronomic Characteristics of Elite Genotypes under Different Water Regimes. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Plants, v. 10, n. 1591, 2021. |
Páginas: |
17 p. |
Idioma: |
Português |
Conteúdo: |
Abstract: Quinoa stands out as an excellent crop in the Cerrado region for cultivation in the offseason or irrigated winter season. Here, we tested the effects of different water regimes on the agronomic characteristics, physiology, and grain quality of different elite quinoa genotypes under field conditions. The experiment was conducted under field conditions at Embrapa Cerrados (Planaltina, DF, Brazil). The experimental design was in randomized blocks, in a split-plot scheme, with four replications. The plots were composed of 18 quinoa genotypes and modified BRS Piabiru (the currently used genotype), and the split-plots were divided into 4 different water regimes. The following variables were evaluated: productivity and productivity per unit of applied water (PUAA), plant height, flavonoids, anthocyanins, gas exchange, chlorophyll, leaf proline, and relative water content. Our results showed that water regimes between 309 and 389 mm can be recommended for quinoa in the Cerrado region. CPAC6 and CPAC13 presented the highest yield and PUAA under high and intermediate WRs, and hence were the most suitable for winter growth under irrigation. CPAC17 is most suitable for off-season growth under rainfed conditions, as it presented the highest PUAA under the low WRs (247 and 150). CPAC9 stood out in terms of accumulation of flavonoids and anthocyanins in all WRs. Physiological analyses revealed different responses of the genotypes to water restriction, together with symptoms of stress under lower water regimes. Our study reinforces the importance of detailed analyses of the relationship between productivity, physiology, and water use when choosing genotypes for planting and harvest in different seasons. MenosAbstract: Quinoa stands out as an excellent crop in the Cerrado region for cultivation in the offseason or irrigated winter season. Here, we tested the effects of different water regimes on the agronomic characteristics, physiology, and grain quality of different elite quinoa genotypes under field conditions. The experiment was conducted under field conditions at Embrapa Cerrados (Planaltina, DF, Brazil). The experimental design was in randomized blocks, in a split-plot scheme, with four replications. The plots were composed of 18 quinoa genotypes and modified BRS Piabiru (the currently used genotype), and the split-plots were divided into 4 different water regimes. The following variables were evaluated: productivity and productivity per unit of applied water (PUAA), plant height, flavonoids, anthocyanins, gas exchange, chlorophyll, leaf proline, and relative water content. Our results showed that water regimes between 309 and 389 mm can be recommended for quinoa in the Cerrado region. CPAC6 and CPAC13 presented the highest yield and PUAA under high and intermediate WRs, and hence were the most suitable for winter growth under irrigation. CPAC17 is most suitable for off-season growth under rainfed conditions, as it presented the highest PUAA under the low WRs (247 and 150). CPAC9 stood out in terms of accumulation of flavonoids and anthocyanins in all WRs. Physiological analyses revealed different responses of the genotypes to water restriction, together with symptoms of st... Mostrar Tudo |
Palavras-Chave: |
Eficiência do uso da água; Troca gasosa. |
Thesagro: |
Chenopodium Quinoa; Composto Fenólico. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/226981/1/Quinoa-for-the-Brazilian-Cerrado-Walter.pdf
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Marc: |
LEADER 02573naa a2200289 a 4500 001 2135368 005 2021-10-15 008 2021 bl uuuu u00u1 u #d 100 1 $aSILVA, P. C. 245 $aQuinoa for the Brazilian Cerrado$bAgronomic Characteristics of Elite Genotypes under Different Water Regimes.$h[electronic resource] 260 $c2021 300 $a17 p. 520 $aAbstract: Quinoa stands out as an excellent crop in the Cerrado region for cultivation in the offseason or irrigated winter season. Here, we tested the effects of different water regimes on the agronomic characteristics, physiology, and grain quality of different elite quinoa genotypes under field conditions. The experiment was conducted under field conditions at Embrapa Cerrados (Planaltina, DF, Brazil). The experimental design was in randomized blocks, in a split-plot scheme, with four replications. The plots were composed of 18 quinoa genotypes and modified BRS Piabiru (the currently used genotype), and the split-plots were divided into 4 different water regimes. The following variables were evaluated: productivity and productivity per unit of applied water (PUAA), plant height, flavonoids, anthocyanins, gas exchange, chlorophyll, leaf proline, and relative water content. Our results showed that water regimes between 309 and 389 mm can be recommended for quinoa in the Cerrado region. CPAC6 and CPAC13 presented the highest yield and PUAA under high and intermediate WRs, and hence were the most suitable for winter growth under irrigation. CPAC17 is most suitable for off-season growth under rainfed conditions, as it presented the highest PUAA under the low WRs (247 and 150). CPAC9 stood out in terms of accumulation of flavonoids and anthocyanins in all WRs. Physiological analyses revealed different responses of the genotypes to water restriction, together with symptoms of stress under lower water regimes. Our study reinforces the importance of detailed analyses of the relationship between productivity, physiology, and water use when choosing genotypes for planting and harvest in different seasons. 650 $aChenopodium Quinoa 650 $aComposto Fenólico 653 $aEficiência do uso da água 653 $aTroca gasosa 700 1 $aRIBEIRO JUNIOR, W. Q. 700 1 $aRAMOS, M. L. G. 700 1 $aCELESTINO, S. M. C. 700 1 $aSILVA, A. N. 700 1 $aCASARI, R. A. C. N. 700 1 $aSANTANA, C. C. 700 1 $aLIMA, C. A. de 700 1 $aWILLIAMS, T. C. R. 700 1 $aVINSON, C. C. 773 $tPlants$gv. 10, n. 1591, 2021.
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