| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Soja. |
|
Data corrente: |
10/02/2009 |
|
Data da última atualização: |
10/02/2009 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
MARTINS, P. K.; JORDÃO, B. Q.; YAMANAKA, N.; FARIAS, J. R. B.; BENEVENTI, M. A.; BINNECK, E.; FUGANTI, R.; STOLF, R.; NEPOMUCENO, A. L. |
|
Afiliação: |
Polyana K. Martins, UEL; Berenice Q. Jordão, UEL; Naoki Yamanaka, JIRCAS; José Renato Bouças Farias. CNPSo; Magda Aparecida Beneventi, UEL; Eliseu Binneck, CNPSo; Renata Fuganti, UEL; Renata Stolf, UEL; Alexandre Lima Nepomuceno, CNPSo. |
|
Título: |
Differential gene expression and mitotic cell analysis of the drought tolerant soybean (Glycine max L. Merrill Fabales, Fabaceae) cultivar MG/BR46 (Conquista) under two water deficit induction systems. |
|
Ano de publicação: |
2008 |
|
Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 31, n. 2, p. 512-521, 2008. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Drought cause serious yield losses in soybean (Glycine max), roots being the first plant organ to detect the water- stress signals triggering defense mechanisms. We used two drought induction systems to identify genes differentially expressed in the roots of the drought-tolerant soybean cultivar MG/BR46 (Conquista) and characterize their expression levels during water deficit. Soybean plants grown in nutrient solution hydroponically and in sand-pots were submitted to water stress and gene expression analysis was conducted using the differential display (DD) and real time polymerase chain reaction (PCR) techniques. Three differentially expressed mRNA transcripts showed homology to the Antirrhinum majus basic helix-loop-helix transcription factor bHLH, the Arabidopsis thaliana phosphatidylinositol transfer protein PITP and the auxin-independent growth regulator 1 (axi 1). The hydroponic experiments showed that after 100 min outside the nutrient solution photosynthesis completely stopped, stomata closed and leaf temperature rose. Both stress induction treatments produced significant decrease in the mitotic indices of root cells. Axi 1, PITP and bHLH were not only differentially expressed during dehydration in the hydroponics experiments but also during induced drought in the pot experiments. Although, there were differences between the two sets of experiments in the time at which up or down regulation occurred, the expression pattern of all three transcripts was related. Similar gene expression and cytological analysis results occurred in both systems, suggesting that hydroponics could be used to simulate drought detection by roots growing in soil and thus facilitate rapid and easy root sampling. MenosDrought cause serious yield losses in soybean (Glycine max), roots being the first plant organ to detect the water- stress signals triggering defense mechanisms. We used two drought induction systems to identify genes differentially expressed in the roots of the drought-tolerant soybean cultivar MG/BR46 (Conquista) and characterize their expression levels during water deficit. Soybean plants grown in nutrient solution hydroponically and in sand-pots were submitted to water stress and gene expression analysis was conducted using the differential display (DD) and real time polymerase chain reaction (PCR) techniques. Three differentially expressed mRNA transcripts showed homology to the Antirrhinum majus basic helix-loop-helix transcription factor bHLH, the Arabidopsis thaliana phosphatidylinositol transfer protein PITP and the auxin-independent growth regulator 1 (axi 1). The hydroponic experiments showed that after 100 min outside the nutrient solution photosynthesis completely stopped, stomata closed and leaf temperature rose. Both stress induction treatments produced significant decrease in the mitotic indices of root cells. Axi 1, PITP and bHLH were not only differentially expressed during dehydration in the hydroponics experiments but also during induced drought in the pot experiments. Although, there were differences between the two sets of experiments in the time at which up or down regulation occurred, the expression pattern of all three transcripts was related. Simila... Mostrar Tudo |
|
Categoria do assunto: |
-- |
|
URL: |
https://www.scielo.br/pdf/gmb/v31n2/a19v31n2.pdf
|
|
Marc: |
LEADER 02469naa a2200217 a 4500
001 1471496
005 2009-02-10
008 2008 bl --- 0-- u #d
100 1 $aMARTINS, P. K.
245 $aDifferential gene expression and mitotic cell analysis of the drought tolerant soybean (Glycine max L. Merrill Fabales, Fabaceae) cultivar MG/BR46 (Conquista) under two water deficit induction systems.
260 $c2008
520 $aDrought cause serious yield losses in soybean (Glycine max), roots being the first plant organ to detect the water- stress signals triggering defense mechanisms. We used two drought induction systems to identify genes differentially expressed in the roots of the drought-tolerant soybean cultivar MG/BR46 (Conquista) and characterize their expression levels during water deficit. Soybean plants grown in nutrient solution hydroponically and in sand-pots were submitted to water stress and gene expression analysis was conducted using the differential display (DD) and real time polymerase chain reaction (PCR) techniques. Three differentially expressed mRNA transcripts showed homology to the Antirrhinum majus basic helix-loop-helix transcription factor bHLH, the Arabidopsis thaliana phosphatidylinositol transfer protein PITP and the auxin-independent growth regulator 1 (axi 1). The hydroponic experiments showed that after 100 min outside the nutrient solution photosynthesis completely stopped, stomata closed and leaf temperature rose. Both stress induction treatments produced significant decrease in the mitotic indices of root cells. Axi 1, PITP and bHLH were not only differentially expressed during dehydration in the hydroponics experiments but also during induced drought in the pot experiments. Although, there were differences between the two sets of experiments in the time at which up or down regulation occurred, the expression pattern of all three transcripts was related. Similar gene expression and cytological analysis results occurred in both systems, suggesting that hydroponics could be used to simulate drought detection by roots growing in soil and thus facilitate rapid and easy root sampling.
700 1 $aJORDÃO, B. Q.
700 1 $aYAMANAKA, N.
700 1 $aFARIAS, J. R. B.
700 1 $aBENEVENTI, M. A.
700 1 $aBINNECK, E.
700 1 $aFUGANTI, R.
700 1 $aSTOLF, R.
700 1 $aNEPOMUCENO, A. L.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 31, n. 2, p. 512-521, 2008.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Soja (CNPSO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 76 | |
| 3. |  | MARTINS, P. K.; DIAS, B. B. A.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Setaria viridis as a model plant for functional genomic studies in C4 Crops. In: KUMAR, S.; BARONE, P.; SMITH, M. Transgenic plants: methods and protocols. Berlim, Alemanha: Springer Science+Business Media, 2019. (Methods in Molecular Biology, v. 1864). p. 49-64| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agroenergia. |
|    |
| 4. | | OYA, T.; MARTINS, P. K.; CASAGRANDE, E. C.; LUGLE, S. M.; NEPOMUCENO, A. L.; NEUMAIER, N.; FARIAS, J. R. B. Agronomic, physiological and molecular characteristics of drought-tolerant Brazilian soybean cultivars. In: EMBRAPA SOYBEAN/JICA-CETAPAR/INTA-EEA/JIRCAS JOINT WORKSHOP ON SOYBEAN IMPROVEMENT, PRODUCTION AND UTILIZATION IN SOUTH AMERICA, 2001, Londrina. Abstracts and program. Londrina: Embrapa Soybean: JIRCAS, 2001. p. 17. (Embrapa Soja. Documentos, 170).| Biblioteca(s): Embrapa Soja. |
| |
| 6. | | MAFRA, V.; MARTINS, P. K.; FRANSCISCO, C. S.; RIBEIRO-ALVES, M.; ASTUA, J. de F.; MACHADO, M. A. Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype. In; INTERNATIONAL RESEARCH CONFERENCE ON HUANGLONGBING , 3., 2013, Orlando, Flórida, USA. IRC HLB III. Proceedings. Orlando, Flórida, USA, 2013, (online).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 9. | | RIBEIRO, A. P.; MARTINS, P. K.; DIAS, B. B. A.; KOBAYASHI, A. K.; ANDRADE, A. C.; MOLINARI, H. B. C. Overexpression of the MATE gene in Setaria viridis. In: INTERNATIONAL SERTARIA GENETICS CONFERENCE, 1., 2014, Beijing, China. [Beijing]: China Agricultural Research Systems, 2014. p. 11.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
| |
| 10. | | MARTINS, P. K.; DIAS, B. B. A.; RIBEIRO, A. P.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Setaria viridis: a tool for functional gene analysis in sugarcane. In: INTERNATIONAL SERTARIA GENETICS CONFERENCE, 1., 2014, Beijing, China. [Beijing]: China Agricultural Research Systems, 2014. p. 19.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
| |
| 11. | | SILVA, M. F. e; CARDINALI, M. C. B.; MARTINS, P. K.; ASTÚA, J. F.; MILORI, D. M. B. P.; MASCARENHAS, Y. P. Aplicação da espectroscopia na região do infravermelho com transformada de fourier (FTIR) no estudo da doença Greening em plantações de citros. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 2., 2010, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação Agropecuária: Embrapa Pecuária Sudeste, 2010. p. 24. (Embrapa Instrumentação Agropecuária. Documentos, 50).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
|  |
| 12. | | SILVA, M. F. e; MILORI, D. M. B. P.; CARDINALI, M. C. B.; MASCARENHAS, Y. P.; MARTINS, P. K.; ASTÚA, J. F.; MACHADO, M. A. Application of fourier transform infrared spectroscopy (FTIR) for early diagnosis of citrus greening disease. Citrus research & technology, Cordeirópolis, v. 31, suplemento, p. 120, 2010. Proceedings of the 18 conference international organization citrus virologists, 2010, Campinas. 1CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
| |
| 13. | | SILVA, M. F. e; MIROLI, D. M. B. P.; CARDINALI, M. C. B.; MASCARENHAS, Y. P.; MARTINS, P. K.; FREITAS ASTUA, J. de; MACHADO, M. A. Application of fourier transform infrared spectroscopy (FTIR) for early diagnosis of citrus greening disease. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 120_PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 14. | | MARTINS, P. K.; JORDÃO, B. Q.; BRETON, M. C.; PEDROSO, J. C.; MARIN, S. R. R.; BINNECK, E.; OYA, T.; NEPOMUCENO, A. L. Avaliação dos efeitos do déficit hídrico na expressão gênica em raízes de soja [Glycine max (l.) Merrill] In: CONGRESSO NACIONAL DE GENÉTICA, 48., 2002, Águas de Lindóia. A genética na inclusão social: resumos. Águas de Lindóia: SBG, 2002. 1 CD-ROM. Resumo Área MG pdf.091.| Biblioteca(s): Embrapa Soja. |
| |
| 15. | | MARTINS, P. K.; DIAS, B. B. A.; RIBEIRO, A. P.; SOUTO, B. de M.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Isolation, in silico analysis and transformation of Setaria viridis with a sugarcane senescence-associated promoter. In: INTERNATIONAL SERTARIA GENETICS CONFERENCE, 1., 2014, Beijing, China. [Beijing]: China Agricultural Research Systems, 2014. p. 25.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
| |
| 16. | | PEDROSO, J. C.; MARTINS, P. K.; BRETON, M. C.; NEPOMUCENO, A. L.; FARIAS J. R. B.; NEUMAIER, N.; SILVA, J. F. V. Identificação de genes diferencialmente expressos em cultivares de soja [Glycine max (L. MerrilL)] e tolerância a infestação de nematóide da galha (Meloidogyne javanica). In: CONGRESSO NACIONAL DE GENÉTICA, 47., 2001, Águas de Lindóia. A genética no século XXI: desafios. [Brasilia]: Sociedade Brasileira de Genética, 2001. 1 CD-ROM. Resumo Área Melhoramento pdf. 1062.| Biblioteca(s): Embrapa Soja. |
| |
| 17. | | PEDROSO, J. C.; MARTINS, P. K.; BRETON, M. C.; SILVA, J. F. V.; NEPOMUCENO, A. L.; FARIAS, J. R.; NEUMAIER, N. Identificação de genes diferencialmente expressos em cultivares de soja [Glycine max (L.) Merrill] e tolerância a infestação de nematóide da galha (Meloidogyne javanica). In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 24., 2002, São Pedro, SP. Resumos... Londrina: Embrapa Soja, 2002. p. 157-158. (Embrapa Soja. Documentos, 185).| Biblioteca(s): Embrapa Soja. |
| |
| 18. | | BRETON, M. C.; MARTINS, P. K.; PEDROSO, J. C.; GOMES, E. V.; RUAS, C. F.; NEUMAIER, N.; NEPOMUCENO, A. L.; OYA, T.; FARIAS, J. R. B. Identificação de genes diferencialmente expressos em soja [Glycine max (L. Merril)] expostas a dois fotoperíodos. In: CONGRESSO NACIONAL DE GENÉTICA, 47., 2001, Águas de Lindóia. A genética no século XXI: desafios. [Brasilia]: Sociedade Brasileira de Genética, 2001. 1 CD-ROM. Resumo Área Melhoramento pdf. 432.| Biblioteca(s): Embrapa Soja. |
| |
| 19. | | DUARTE, K. E.; VIEIRA, N. G.; MARTINS, P. K.; RIBEIRO, A. P.; DIAS, B. B. A.; MOLINARI, H. B. C.; KOBAYASHI, A. K.; MARRACCINI, P.; ANDRADE, A. C. Identificação e caracterização de genes órfãos ("no hits") de café (Coffea Canephora), envolvidos na resposta à seca. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 8., 2013, Salvador, BA. Anais...Brasília: Consórcio Pesquisa Café, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
| |
| 20. | | VENANCIO, A. L.; CARDINALLI, M. C. B.; SILVA, M. F.; MILORI, D. M. B. P.; FERREIRA, E. J.; MARTINS, P. K.; FREITAS-ASTÚA, J. Avaliação da precocidade do diagnóstico do Greening utilizando espectroscopia de fluorescência induzida por laser (LIFS). In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 2., 2010, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação Agropecuária: Embrapa Pecuária Sudeste, 2010. p. 36. (Embrapa Instrumentação Agropecuária. Documentos, 50).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
| |
| Registros recuperados : 76 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
