Data/hora: 24/06/2024 05:47:51

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 09/09/1993
Data da última atualização: | 09/09/1993
Autoria: | MORENO, P.; GUERRI, J.; BALLESTER-OLMOS, J. F.; MARTINEZ, M. E.
Afiliação: | Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial, 46113 Moncada, Valencia, Spain.
Título: | Segregation of citrus tristeza virus strains evidenced by double stranded RNA (dsRNA) analysis
Ano de publicação: | 1991
Fonte/Imprenta: | Riverside, California: International Organization of Citrus Virologists, 1991.
Páginas: | p.20-23
Idioma: | Inglês
Notas: | Conference of the International Organization of citrus virologists, 11., 1991, Riverside California, Proceedings...
Conteúdo: | A field isolate of citrus tristeza virus (CTV), T-385, was separated from other virus ans virus-like diseaes by aphid transmission to Mexican lime seedlings. This isolate did not cause decline of sweet orange on sour orange rootstock under field conditions and it induced mild vein clearing and an inconspicuous stem pitting on Mexican lime. Two months after aphid transmission, one of the infected lime seedlings was used as an inoculum source to graft-inoculate 40 citrom plants.Two years later, the citron plants were analysed individually for dsNRA content and up to 23 different electrophoretic profiles were found. DsNRA analysis has been repeated three times through the year and the individual patterns remained unchanged. Thirteen of these CTV sub-isolates weregraft-transmitted to sweet orange seedlings and the corresponding dsNRA profiles were identical to those previously obtained from citron. All these subsolates induced mild symptoms of Mexican lime similar to those of the original isolate (T-385). These results substantiate that several CTV strains can infect a single citrus plant.
Palavras-Chave: | Silver stain; Symptoms.
Thesaurus Nal: | electrophoresis.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (13268-1) - ADC - PL - -- - 634.3040601 - C748p - 1992000183 - DIS