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Registro Completo |
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
14/07/2020 |
Data da última atualização: |
14/07/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MARTINEZ, H. E. P.; SOUZA, B. P. de; CAIXETA, E. T.; CARVALHO, F. P. de; CLEMENTE, J. M. |
Afiliação: |
HERMINIA E. P. MARTINEZ, DEPARTAMENT OF AGRONOMY - UFV; BRUNA P. DE SOUZA, DEPARTAMENT OF AGRONOMY - UFV; EVELINE TEIXEIRA CAIXETA MOURA, CNPCa; FELIPE P. DE CARVALHO, DEPARTAMENT OF AGRONOMY - UFV; JUNIA M. CLEMENTE, DEPARTAMENT OF AGRONOMY - UFV. |
Título: |
Water deficit changes nitrate uptake and expression of some nitrogen related genes in coffee-plants (Coffea arabica L.). |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Scientia Horticulturae, v. 267, 109254, 2020. |
DOI: |
https://doi.org/10.1016/j.scienta.2020.109254 |
Idioma: |
Inglês |
Conteúdo: |
Brazilian coffee cultivation has been expanding into areas of low fertility soils, many times subjected to low water availability, where the use of nitrogen fertilizers is high. To evaluate the effect of water stress on nitrate uptake and on the expression of genes that encode the nitrate uptake and assimilation we have done three experiments with Coffea arabica L. plants. The first was an 8 × 2 factorial (eight coffee cultivars x with or without water stress), with three replicates. We obtained the kinetic parameters of nitrate uptake. The second and third experiments consisted of 2 × 2 (two cultivars x with or without water stress) factorials. One of them was conducted with adequate supply of nitrogen and the other with omission of N. The transcriptional profiles of the genes NRT1.2, NRT3.2, NIA2, GLT, and GLN1.3 were analyzed. Water stress decreased Vmax (maximum velocity of absorption), Km (Michaelis-Menten constant), and Cmin (external concentration at which net uptake of ions is zero) in all cultivars. The cultivar Catuaí Amarelo IAC 62 stood out with high Vmax and low Km in both conditions, and the Cultivar Mundo Novo IAC379-19, although with low Vmax, had low Km and low Cmin, without changes under water stress. In plants under water stress, the genes NRT1.2, NRT3.2, NIA2, and GLT showed higher differential transcription in root systems. GLN1.3 gene had higher relative expression in the shoots. Under water stress, the relative expression of these genes was higher in Mundo Novo IAC 379-19 than in Catuaí Amarelo IAC 62 in both nitrogen doses. MenosBrazilian coffee cultivation has been expanding into areas of low fertility soils, many times subjected to low water availability, where the use of nitrogen fertilizers is high. To evaluate the effect of water stress on nitrate uptake and on the expression of genes that encode the nitrate uptake and assimilation we have done three experiments with Coffea arabica L. plants. The first was an 8 × 2 factorial (eight coffee cultivars x with or without water stress), with three replicates. We obtained the kinetic parameters of nitrate uptake. The second and third experiments consisted of 2 × 2 (two cultivars x with or without water stress) factorials. One of them was conducted with adequate supply of nitrogen and the other with omission of N. The transcriptional profiles of the genes NRT1.2, NRT3.2, NIA2, GLT, and GLN1.3 were analyzed. Water stress decreased Vmax (maximum velocity of absorption), Km (Michaelis-Menten constant), and Cmin (external concentration at which net uptake of ions is zero) in all cultivars. The cultivar Catuaí Amarelo IAC 62 stood out with high Vmax and low Km in both conditions, and the Cultivar Mundo Novo IAC379-19, although with low Vmax, had low Km and low Cmin, without changes under water stress. In plants under water stress, the genes NRT1.2, NRT3.2, NIA2, and GLT showed higher differential transcription in root systems. GLN1.3 gene had higher relative expression in the shoots. Under water stress, the relative expression of these genes was higher in M... Mostrar Tudo |
Thesagro: |
Café; Fixação de Nitrogênio; Potencial Hídrico; Sistema de Cultivo. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02324naa a2200229 a 4500 001 2123834 005 2020-07-14 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.scienta.2020.109254$2DOI 100 1 $aMARTINEZ, H. E. P. 245 $aWater deficit changes nitrate uptake and expression of some nitrogen related genes in coffee-plants (Coffea arabica L.).$h[electronic resource] 260 $c2020 520 $aBrazilian coffee cultivation has been expanding into areas of low fertility soils, many times subjected to low water availability, where the use of nitrogen fertilizers is high. To evaluate the effect of water stress on nitrate uptake and on the expression of genes that encode the nitrate uptake and assimilation we have done three experiments with Coffea arabica L. plants. The first was an 8 × 2 factorial (eight coffee cultivars x with or without water stress), with three replicates. We obtained the kinetic parameters of nitrate uptake. The second and third experiments consisted of 2 × 2 (two cultivars x with or without water stress) factorials. One of them was conducted with adequate supply of nitrogen and the other with omission of N. The transcriptional profiles of the genes NRT1.2, NRT3.2, NIA2, GLT, and GLN1.3 were analyzed. Water stress decreased Vmax (maximum velocity of absorption), Km (Michaelis-Menten constant), and Cmin (external concentration at which net uptake of ions is zero) in all cultivars. The cultivar Catuaí Amarelo IAC 62 stood out with high Vmax and low Km in both conditions, and the Cultivar Mundo Novo IAC379-19, although with low Vmax, had low Km and low Cmin, without changes under water stress. In plants under water stress, the genes NRT1.2, NRT3.2, NIA2, and GLT showed higher differential transcription in root systems. GLN1.3 gene had higher relative expression in the shoots. Under water stress, the relative expression of these genes was higher in Mundo Novo IAC 379-19 than in Catuaí Amarelo IAC 62 in both nitrogen doses. 650 $aCafé 650 $aFixação de Nitrogênio 650 $aPotencial Hídrico 650 $aSistema de Cultivo 700 1 $aSOUZA, B. P. de 700 1 $aCAIXETA, E. T. 700 1 $aCARVALHO, F. P. de 700 1 $aCLEMENTE, J. M. 773 $tScientia Horticulturae$gv. 267, 109254, 2020.
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Embrapa Café (CNPCa) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
19/11/2015 |
Data da última atualização: |
15/03/2016 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
FERREIRA, A. S.; ZUCARELI, C.; BALBINOT JUNIOR, A. A.; WERNER, F.; COELHO, A. E. |
Afiliação: |
UEL; UEL; ALVADI ANTONIO BALBINOT JUNIOR, CNPSO; Embrapa Soja; UFSC. |
Título: |
Tamanho de sementes de soja em resposta a diferentes densidades de plantas. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Informativo ABRATES, Brasília, v. 25, n.2, set. 2015. |
Idioma: |
Português |
Notas: |
Edição de Anais d XIX Congresso Brasileiro de Sementes, Foz do Iguaçu, 2015. |
Thesagro: |
Semente. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00591nam a2200169 a 4500 001 2028974 005 2016-03-15 008 2015 bl uuuu u00u1 u #d 100 1 $aFERREIRA, A. S. 245 $aTamanho de sementes de soja em resposta a diferentes densidades de plantas.$h[electronic resource] 260 $aInformativo ABRATES, Brasília, v. 25, n.2, set. 2015.$c2015 500 $aEdição de Anais d XIX Congresso Brasileiro de Sementes, Foz do Iguaçu, 2015. 650 $aSemente 700 1 $aZUCARELI, C. 700 1 $aBALBINOT JUNIOR, A. A. 700 1 $aWERNER, F. 700 1 $aCOELHO, A. E.
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