Data/hora: 23/06/2024 04:19:11

Biblioteca(s): | Embrapa Meio Ambiente.
Data corrente: | 09/12/2019
Data da última atualização: | 10/06/2022
Autoria: | MARTINEZ, A. F. C.; MELLO, F. M. P.; ZUCCHI, T. D.; MELO, I. S. de; MORAES, L. A. B.
Afiliação: | ANA FLÁVIA CANOVAS MARTINEZ, FFCLRP-USP; FLÁVIA MADOLESI PEREIRA MELLO; TIAGO DOMINGUES ZUCCHI; ITAMAR SOARES DE MELO, CNPMA; LUIZ ALBERTO BERALDO MORAES, FFCLRP-USP.
Título: | Tandem mass spectrometry methods to accelerate the identification of phytotoxic metabolites produced by Streptomyces sp. 39 PL.
Ano de publicação: | 2019
Fonte/Imprenta: | Natural Product Research, v. 34, n. 2, p. 210-216, 2019.
DOI: | https://doi.org/10.1080/14786419.2018.1525713
Idioma: | Inglês
Notas: | Arquivo com texto completo contém a versão publicada online, sem dados de volume e paginação.
Conteúdo: | Abstract: Natural products isolated by microorganisms are interesting in the search for new compounds with several biological activities. However, low concentration and structural diversity make the isolation a time-consuming step. Tandem mass spectrometry is a well-established technology for the identification and characterization of target microbial natural products due to high sensitivity and selectivity of these experiments. We developed a method employing neutral loss experiments (LC-ESI-MS/MS) to identify luminacins in microbial crude extracts. The luminacins class exhibited conserved fragmentation pattern with loss at 172Da relative to glycosides fragment and this loss was used in searching for compounds belonging to this class. Therefore, the crude extract produced by Streptomyces sp. 39 PL was analysed and five luminacins were isolated - one is a novel luminacin I at 466Da.
Palavras-Chave: | Dereplication; Luminacins; Neutral loss experiments.
Thesagro: | Análise Química; Bactéria; Identificação; Método de Análise.
Thesaurus Nal: | phytotoxins; Species identification; Streptomyces; Tandem mass spectrometry.
Categoria do assunto: | S Ciências Biológicas
Registro original: | Embrapa Meio Ambiente (CNPMA)
Exemplares: | CNPMA - (16579-1) - UPC - AP - DD -  -  -  - DIS