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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação; Embrapa Pecuária Sudeste. |
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Data corrente: |
10/03/2022 |
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Data da última atualização: |
08/11/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TADINI, A. M.; GORANOV, A. I.; MARTIN NETO, L.; BERNARDI, A. C. de C.; OLIVEIRA, P. P. A.; PEZZOPANE, J. R. M.; HATCHER, P. G. |
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Afiliação: |
ANA MARIA TADINI, USP; ALEKSANDAR I. GORANOV, Old Dominion University; LADISLAU MARTIN NETO, CNPDIA; ALBERTO CARLOS DE CAMPOS BERNARDI, CPPSE; PATRICIA PERONDI ANCHAO OLIVEIRA, CPPSE; JOSE RICARDO MACEDO PEZZOPANE, CPPSE; PATRICK G. HATCHER, Old Dominion University. |
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Título: |
Structural characterization using 2D NMR spectroscopy andTMAH-GC × GC-MS: application to humic acids from soils of anintegrated agricultural system and an Atlantic native forest. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Science of The Total Environment, v.815, apr. 2022, 152605. |
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Páginas: |
14 p. |
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DOI: |
https://doi.org/10.1016/j.scitotenv.2021.152605 |
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Idioma: |
Inglês |
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Conteúdo: |
Understanding the chemical make-up of soils and their structure is critical for constraining the role of soil organic mat-ter (SOM) into the global biogeochemical cycles, as well as to understand the capability of SOM to sequester carbonand mitigate greenhouse gas emissions. Here, we use two-dimensional1H-13C heteronuclear single quantum coher-encenuclear magnetic resonance (2D1H-13C HSQC NMR) spectroscopy to structurally characterize the most refractorycomponent of SOM, the humic acid (HA). The observations from 2D1H-13C HSQC NMR were coupled with lignin phe-nol and fatty acid measurements using tetramethylammonium hydroxide (TMAH) thermochemolysis?two-dimensional gas chromatography?mass spectrometry (TMAH-GC × GC-MS). We studied humic acids extractedfrom an integrated Crop?Livestock?Forest System (CLFS) agricultural area and an undisturbed Atlantic Native Forest(NF) area. We evaluated soils from two different depths: the topsoil (0?20 cm) and subsoil (60?100 cm) layers, andreveal the presence of oxidized ligninaceous phenols as we had previously hypothesized. Collectively, our results indi-cate that there are significant oxidative processes with increasing soil depth which are more pronounced in the CLFSrelative to the NF area. Degradation of stearic acid with increasing depth in the CLFS soils indicated that the CLFS ismore microbiologically active than NF. Therefore, CLFS is less biochemically stable than we originally perceived.The enhanced bio-reactivity of CLFS is likely driving the enhanced carbon sequestration in the CLFS soils. This is perhaps dueto the diversityof biomass remnants available at the CLFS soil rhizosphere which allowsfor more differenttypes of biomass to be sequestered as oxidized ligninaceous phenols. Our results employing structural characterizationwith 2D1H-13C HSQC NMR and TMAH-GC × GC-MS provide a new layer of knowledge about the practice of inte-grated agricultural systems and allow us to understand the structure and fate of sequestered carbon in soils from dif-ferent soil environments. MenosUnderstanding the chemical make-up of soils and their structure is critical for constraining the role of soil organic mat-ter (SOM) into the global biogeochemical cycles, as well as to understand the capability of SOM to sequester carbonand mitigate greenhouse gas emissions. Here, we use two-dimensional1H-13C heteronuclear single quantum coher-encenuclear magnetic resonance (2D1H-13C HSQC NMR) spectroscopy to structurally characterize the most refractorycomponent of SOM, the humic acid (HA). The observations from 2D1H-13C HSQC NMR were coupled with lignin phe-nol and fatty acid measurements using tetramethylammonium hydroxide (TMAH) thermochemolysis?two-dimensional gas chromatography?mass spectrometry (TMAH-GC × GC-MS). We studied humic acids extractedfrom an integrated Crop?Livestock?Forest System (CLFS) agricultural area and an undisturbed Atlantic Native Forest(NF) area. We evaluated soils from two different depths: the topsoil (0?20 cm) and subsoil (60?100 cm) layers, andreveal the presence of oxidized ligninaceous phenols as we had previously hypothesized. Collectively, our results indi-cate that there are significant oxidative processes with increasing soil depth which are more pronounced in the CLFSrelative to the NF area. Degradation of stearic acid with increasing depth in the CLFS soils indicated that the CLFS ismore microbiologically active than NF. Therefore, CLFS is less biochemically stable than we originally perceived.The enhanced bio-reactivity of CLFS is lik... Mostrar Tudo |
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Palavras-Chave: |
2D1H 13CHSQCNM; ILPF; Lignin phenols; TMAH thermochemolysis. |
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Thesaurus Nal: |
Humic acids; Integrated agricultural systems; Soil organic matter. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 03066naa a2200301 a 4500
001 2140710
005 2023-11-08
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.scitotenv.2021.152605$2DOI
100 1 $aTADINI, A. M.
245 $aStructural characterization using 2D NMR spectroscopy andTMAH-GC × GC-MS$bapplication to humic acids from soils of anintegrated agricultural system and an Atlantic native forest.$h[electronic resource]
260 $c2022
300 $a14 p.
520 $aUnderstanding the chemical make-up of soils and their structure is critical for constraining the role of soil organic mat-ter (SOM) into the global biogeochemical cycles, as well as to understand the capability of SOM to sequester carbonand mitigate greenhouse gas emissions. Here, we use two-dimensional1H-13C heteronuclear single quantum coher-encenuclear magnetic resonance (2D1H-13C HSQC NMR) spectroscopy to structurally characterize the most refractorycomponent of SOM, the humic acid (HA). The observations from 2D1H-13C HSQC NMR were coupled with lignin phe-nol and fatty acid measurements using tetramethylammonium hydroxide (TMAH) thermochemolysis?two-dimensional gas chromatography?mass spectrometry (TMAH-GC × GC-MS). We studied humic acids extractedfrom an integrated Crop?Livestock?Forest System (CLFS) agricultural area and an undisturbed Atlantic Native Forest(NF) area. We evaluated soils from two different depths: the topsoil (0?20 cm) and subsoil (60?100 cm) layers, andreveal the presence of oxidized ligninaceous phenols as we had previously hypothesized. Collectively, our results indi-cate that there are significant oxidative processes with increasing soil depth which are more pronounced in the CLFSrelative to the NF area. Degradation of stearic acid with increasing depth in the CLFS soils indicated that the CLFS ismore microbiologically active than NF. Therefore, CLFS is less biochemically stable than we originally perceived.The enhanced bio-reactivity of CLFS is likely driving the enhanced carbon sequestration in the CLFS soils. This is perhaps dueto the diversityof biomass remnants available at the CLFS soil rhizosphere which allowsfor more differenttypes of biomass to be sequestered as oxidized ligninaceous phenols. Our results employing structural characterizationwith 2D1H-13C HSQC NMR and TMAH-GC × GC-MS provide a new layer of knowledge about the practice of inte-grated agricultural systems and allow us to understand the structure and fate of sequestered carbon in soils from dif-ferent soil environments.
650 $aHumic acids
650 $aIntegrated agricultural systems
650 $aSoil organic matter
653 $a2D1H 13CHSQCNM
653 $aILPF
653 $aLignin phenols
653 $aTMAH thermochemolysis
700 1 $aGORANOV, A. I.
700 1 $aMARTIN NETO, L.
700 1 $aBERNARDI, A. C. de C.
700 1 $aOLIVEIRA, P. P. A.
700 1 $aPEZZOPANE, J. R. M.
700 1 $aHATCHER, P. G.
773 $tScience of The Total Environment$gv.815, apr. 2022, 152605.
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Embrapa Pecuária Sudeste (CPPSE) |
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| 1. |  | LIMA FILHO, J. M. P.; ASSIS, J. S. de; TEIXEIRA, A. H. de C.; CUNHA, G. A. P. D.; CASTRO NETO, M. T. de. Ecofisiologia. In: GENU, P. J. de C.; PINTO, A. C. de Q. (Ed.). A cultura da mangueira. Brasília, DF: Embrapa Informação Tecnológica, 2002. cap. 3, p. 37-49 il.| Tipo: Capítulo em Livro Técnico-Científico |
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