Data/hora: 22/06/2024 06:46:06

Biblioteca(s): | Embrapa Trigo.
Data corrente: | 10/01/2010
Data da última atualização: | 22/02/2013
Autoria: | CHUNWONGSE, J.; MARTIN, G. B.; TANKSLEY, S. D.
Título: | Pre-germination genotypic screening using PCR amplification of half-seeds.
Ano de publicação: | 1993
Fonte/Imprenta: | Theoretical and Applied Genetics, v. 86, p. 694-698, 1993.
Idioma: | Inglês
Conteúdo: | A simple and rapid PCR-based method has been developed for determining the genotype of seeds before germination. Single half-seeds of rice (Oryza sativa L.) and wheat (Triticum aestivum L. em. Thell.) were preincubated, without grinding, in an aqueous extraction buffer. The resulting supernatants were then used in polymerase chain reaction (PCR) with oligonucleotide primers corresponding to rice single-copy sequences or a wheat microsatellite repeat. PCR products of identical size were amplified using either the half-seed extract or DNA isolated from leaf tissue. The remnant half-seeds can be maintained in ordered arrays using microtiter plates allowing the recovery of selected genotypes. Pre-germination genotypic screening of seed populations as described in this report should be useful for a variety of applications in plant breeding and genetics studies.
Palavras-Chave: | extração de DNA; PCR; RAPD; RFLP; Seleção assistida por marcadores.
Categoria do assunto: | --
Registro original: | Embrapa Trigo (CNPT)
Exemplares: | CNPT - (28494-1) - ADD - SP - -- - 14575 -  - 14575 - DIS