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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
09/11/2006 |
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Data da última atualização: |
13/06/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
NOGUEIRA, A. R. de A.; OLIVEIRA, D. B.; CARAPELI, R.; FERRAZ, L. F. de M.; MARTELLI, P. B. |
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Afiliação: |
ANA RITA DE ARAUJO NOGUEIRA, CPPSE; DANIEL B. OLIVEIRA, UFSCar/SÃO CARLOS, SP.; RODOLFO CARAPELI, UFSCar/SÃO CARLOS, SP.; LUIZ FRANCISCO DE MATTEO FERRAZ, CNPDIA; PATRICIA B. MARTELLI, UNIVERSIDADE DE SÃO JOÃO DEL REI, MG. |
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Título: |
Multicomutation flow system for spectrophometric total amino acids determination in plant material. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE FLOW ANALYSIS, 10., 2006, Porto. Proceedings... Porto: IAFA, 2006 |
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Páginas: |
p.183 |
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Idioma: |
Português |
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Conteúdo: |
To identify lhe nitrogen transportation forms and attain the control mechanism, the amount of free amino acids in different parts of lhe plant has to be determined. An automatic, fast and reliable procedure multicomutated flow system {1] has been developed for spectrophotometric analysis of totalamino acids in plant material. The method is suitable for routine analysis for a large number of samples of plant material. The flow manifold was designed with computer-controlled three-way solenoid valves for independent handling of sample and reagent solutions and a data acquisition system from a spectrophotometer, employed for signal measurements. The software for system control was performed bya program with use of a LabView platform INationallntruments) [2]. The detection reaction was based on the complexation of amino functional groups of amino acids by ninhydrin. It reacts with free a-amino groups, producing lhe colored ninhydrin chromophore called Ruhemann's purple IRP) IAm". = 570 nm; E = 22 000) [3]. The proposed detection system shows a linear range concentration up to 2.0 X 10-3 mal L-I with coefficient of variation of 1.1% (n = 101. Detection limits were estimated as 2.8 x 10-3 mal L-I at 99.7% confidence level for total amino acids. and a mean sampling rale of 30 determinations per hour was achieved. |
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Palavras-Chave: |
Flow system; Multicomutation. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/39117/1/PROCIARAN2006.00095.pdf
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Marc: |
LEADER 01941nam a2200193 a 4500
001 1047591
005 2023-06-13
008 2006 bl uuuu u00u1 u #d
100 1 $aNOGUEIRA, A. R. de A.
245 $aMulticomutation flow system for spectrophometric total amino acids determination in plant material.
260 $aIn: INTERNATIONAL CONFERENCE FLOW ANALYSIS, 10., 2006, Porto. Proceedings... Porto: IAFA$c2006
300 $ap.183
520 $aTo identify lhe nitrogen transportation forms and attain the control mechanism, the amount of free amino acids in different parts of lhe plant has to be determined. An automatic, fast and reliable procedure multicomutated flow system {1] has been developed for spectrophotometric analysis of totalamino acids in plant material. The method is suitable for routine analysis for a large number of samples of plant material. The flow manifold was designed with computer-controlled three-way solenoid valves for independent handling of sample and reagent solutions and a data acquisition system from a spectrophotometer, employed for signal measurements. The software for system control was performed bya program with use of a LabView platform INationallntruments) [2]. The detection reaction was based on the complexation of amino functional groups of amino acids by ninhydrin. It reacts with free a-amino groups, producing lhe colored ninhydrin chromophore called Ruhemann's purple IRP) IAm". = 570 nm; E = 22 000) [3]. The proposed detection system shows a linear range concentration up to 2.0 X 10-3 mal L-I with coefficient of variation of 1.1% (n = 101. Detection limits were estimated as 2.8 x 10-3 mal L-I at 99.7% confidence level for total amino acids. and a mean sampling rale of 30 determinations per hour was achieved.
653 $aFlow system
653 $aMulticomutation
700 1 $aOLIVEIRA, D. B.
700 1 $aCARAPELI, R.
700 1 $aFERRAZ, L. F. de M.
700 1 $aMARTELLI, P. B.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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