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 | Acesso ao texto completo restrito à biblioteca da Embrapa Café. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Café. |
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Data corrente: |
19/09/2017 |
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Data da última atualização: |
03/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
KOBAYASHI, A. K.; VIEIRA, L. G. E.; BESPALHOK FILHO, J. C.; LEITE JUNIOR, R. P.; PEREIRA, L. F. P.; MOLINARI, H. B. C.; MARQUES, V. V. |
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Afiliação: |
ADILSON KENJI KOBAYASHI, CNPAE; LUIZ GONZAGA E. VIEIRA; JOÃO CARLOS BESPALHOK FILHO; RUI PEREIRA LEITE JUNIOR; LUIZ FILIPE P. PEREIRA; HUGO BRUNO CORREA MOLINARI, CNPAE; VIVIANI V. MARQUES. |
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Título: |
Enhanced resistance to citrus canker in transgenic sweet orange expressing the sarcotoxin IA gene. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
European Journal of Plant Pathology, v. 149, n. 4, p. 865-873, 2017. |
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DOI: |
10.1007/s10658-017-1234-5 |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus canker, caused by the bacterial pathogen Xanthomonas citri subp. Citri (Xcc), is a serious disease reported in most citrus-producing areas around the world. Although different levels of field resistance to citrus canker have been reported in sweet oranges, they are usually not sufficient to provide adequate control of the disease. Ectopic over-expression of antibacterial genes is one of the potential strategies to increase plant resistance to bacterial diseases. Previous in vitro results showed that sarcotoxin IA, an antimicrobial peptide isolated from the flesh fly (Sarcophaga peregrina), can be efficient to control different plant pathogenic bacteria, including Xcc. Transgenic BPera^ sweet orange (Citrus sinensis [L.] Osbeck) plants constitutively expressing the sarcotoxin IA peptide fused to the PR1a signal peptide from Nicotiana tabacum for secretion in the intercellular space were obtained by Agrobacteriummediated transformation using thin sections of mature explants. Citrus canker resistance evaluation in leaves of transgenic and non-transgenic plants was performed through inoculations with Xcc by infiltration and spraying. The Xcc population was up to 2 log unit lower in leaves of the transgenic plants compared to those of non-transgenic controls. Incidence of canker lesions was significantly higher in non-transformed controls (>10 lesions/cm2) than in the transgenic plants (<5 lesions/ cm2) after injection infiltration or spraying with Xcc inoculum. Accumulation of sarcotoxin IA peptide in sweet orange tissue did not cause any deleterious effects on the growth and development of the transgenic plants, indicating this approach is suitable to provide resistance to citrus canker. MenosCitrus canker, caused by the bacterial pathogen Xanthomonas citri subp. Citri (Xcc), is a serious disease reported in most citrus-producing areas around the world. Although different levels of field resistance to citrus canker have been reported in sweet oranges, they are usually not sufficient to provide adequate control of the disease. Ectopic over-expression of antibacterial genes is one of the potential strategies to increase plant resistance to bacterial diseases. Previous in vitro results showed that sarcotoxin IA, an antimicrobial peptide isolated from the flesh fly (Sarcophaga peregrina), can be efficient to control different plant pathogenic bacteria, including Xcc. Transgenic BPera^ sweet orange (Citrus sinensis [L.] Osbeck) plants constitutively expressing the sarcotoxin IA peptide fused to the PR1a signal peptide from Nicotiana tabacum for secretion in the intercellular space were obtained by Agrobacteriummediated transformation using thin sections of mature explants. Citrus canker resistance evaluation in leaves of transgenic and non-transgenic plants was performed through inoculations with Xcc by infiltration and spraying. The Xcc population was up to 2 log unit lower in leaves of the transgenic plants compared to those of non-transgenic controls. Incidence of canker lesions was significantly higher in non-transformed controls (>10 lesions/cm2) than in the transgenic plants (<5 lesions/ cm2) after injection infiltration or spraying with Xcc inoculum. Accumulati... Mostrar Tudo |
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Palavras-Chave: |
Bacterial disease resistance; Citrus canker; Mature tissue transformation. |
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Thesagro: |
Agrobacterium Tumefaciens. |
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Thesaurus Nal: |
Agrobacterium radiobacter; Antimicrobial peptides. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02638naa a2200277 a 4500
001 2075843
005 2024-01-03
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1007/s10658-017-1234-5$2DOI
100 1 $aKOBAYASHI, A. K.
245 $aEnhanced resistance to citrus canker in transgenic sweet orange expressing the sarcotoxin IA gene.$h[electronic resource]
260 $c2017
520 $aCitrus canker, caused by the bacterial pathogen Xanthomonas citri subp. Citri (Xcc), is a serious disease reported in most citrus-producing areas around the world. Although different levels of field resistance to citrus canker have been reported in sweet oranges, they are usually not sufficient to provide adequate control of the disease. Ectopic over-expression of antibacterial genes is one of the potential strategies to increase plant resistance to bacterial diseases. Previous in vitro results showed that sarcotoxin IA, an antimicrobial peptide isolated from the flesh fly (Sarcophaga peregrina), can be efficient to control different plant pathogenic bacteria, including Xcc. Transgenic BPera^ sweet orange (Citrus sinensis [L.] Osbeck) plants constitutively expressing the sarcotoxin IA peptide fused to the PR1a signal peptide from Nicotiana tabacum for secretion in the intercellular space were obtained by Agrobacteriummediated transformation using thin sections of mature explants. Citrus canker resistance evaluation in leaves of transgenic and non-transgenic plants was performed through inoculations with Xcc by infiltration and spraying. The Xcc population was up to 2 log unit lower in leaves of the transgenic plants compared to those of non-transgenic controls. Incidence of canker lesions was significantly higher in non-transformed controls (>10 lesions/cm2) than in the transgenic plants (<5 lesions/ cm2) after injection infiltration or spraying with Xcc inoculum. Accumulation of sarcotoxin IA peptide in sweet orange tissue did not cause any deleterious effects on the growth and development of the transgenic plants, indicating this approach is suitable to provide resistance to citrus canker.
650 $aAgrobacterium radiobacter
650 $aAntimicrobial peptides
650 $aAgrobacterium Tumefaciens
653 $aBacterial disease resistance
653 $aCitrus canker
653 $aMature tissue transformation
700 1 $aVIEIRA, L. G. E.
700 1 $aBESPALHOK FILHO, J. C.
700 1 $aLEITE JUNIOR, R. P.
700 1 $aPEREIRA, L. F. P.
700 1 $aMOLINARI, H. B. C.
700 1 $aMARQUES, V. V.
773 $tEuropean Journal of Plant Pathology$gv. 149, n. 4, p. 865-873, 2017.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Biblioteca |
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Origem |
Tipo/Formato |
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Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
28/03/2022 |
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Data da última atualização: |
28/03/2022 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SOUSA, S. G. A. de; PERIN, R.; COSTA, J. R. da; WANDELLI, E. V.; MACEDO, J. L. V. de. |
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Afiliação: |
SILAS GARCIA AQUINO DE SOUSA, CPAA; ROGERIO PERIN, CPAA; JOANNE REGIS DA COSTA, CPAA; ELISA VIEIRA WANDELLI, CPAA; JEFERSON LUIS VASCONCELOS DE MACEDO, CPAA. |
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Título: |
Aspectos financeiros de um sistema agrossilvicultural no estado do Amazonas. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 6., 2006, Campos dos Goytacazes. Bases científicas para o desenvolvimento sustentável: resumos expandidos. Campo de Goytacazes: Universidade Estadual do Norte Fluminense Darcy Ribeiro; Salvador: Sociedade Brasileira de Sistemas Agroflorestais, 2006. |
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Idioma: |
Português |
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Conteúdo: |
O presente trabalho é uma síntese das análises econômicas e financeiras realizada no âmbito do projeto de pesquisa com sistemas agroflorestais implantadas em áreas degradadas de pastagens, no campo experimental da Embrapa Amazônia Ocidental. |
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Palavras-Chave: |
Agrofloresta. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/233045/1/trabalho249.pdf
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Marc: |
LEADER 01013nam a2200169 a 4500
001 2141443
005 2022-03-28
008 2006 bl uuuu u00u1 u #d
100 1 $aSOUSA, S. G. A. de
245 $aAspectos financeiros de um sistema agrossilvicultural no estado do Amazonas.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 6., 2006, Campos dos Goytacazes. Bases científicas para o desenvolvimento sustentável: resumos expandidos. Campo de Goytacazes: Universidade Estadual do Norte Fluminense Darcy Ribeiro; Salvador: Sociedade Brasileira de Sistemas Agroflorestais$c2006
520 $aO presente trabalho é uma síntese das análises econômicas e financeiras realizada no âmbito do projeto de pesquisa com sistemas agroflorestais implantadas em áreas degradadas de pastagens, no campo experimental da Embrapa Amazônia Ocidental.
653 $aAgrofloresta
700 1 $aPERIN, R.
700 1 $aCOSTA, J. R. da
700 1 $aWANDELLI, E. V.
700 1 $aMACEDO, J. L. V. de
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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