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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
17/05/2012 |
Data da última atualização: |
20/02/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CARDOSO, F. C.; MARQUES, R.; BOTOSSO, P. C.; MARQUES, M. C. M. |
Afiliação: |
FERNANDA C. CARDOSO, UFPR; RENATO MARQUES, UFPR; PAULO CESAR BOTOSSO, CNPF; M. C. M. Marques, UFPR. |
Título: |
Stem growth and phenology of two tropical trees in contrasting soil conditions. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Plant and Soil, n. 354, p. 269-281, 2012. |
Idioma: |
Inglês |
Conteúdo: |
Background and aims Phenological variations in tropical forests are usually explained by climate. Nevertheless, considering that soil water availability and nutrient content also influence plant water status and metabolism, soil conditions may also be important in the regulation of plant reproductive and vegetative activities over time.We investigated whether phenological patterns and stem growth differ in trees growing in two types of soil that display contrasting water and nutrient availability, namely, Gleysol (moist and nutrient-poor) and Cambisol (drier and nutrient rich). Methods Phenological observations (flushing, leaf fall, flowering and fruiting) and stem diameter growth were recorded for 120 trees fitted with fixed dendrometer bands, at 15 days intervals, for 1 year. Two species of contrasting deciduousness were investigated: Senna multijuga (semi-deciduous) and Citharexylum myrianthum (deciduous). Results Both species were seasonal in all phenophases, regardless of soil type. However, frequency, mean date and intensity of phenophases varied according to soil type. Girth increment of C. myrianthum was four times greater in Cambisol than in Gleysol, whereas the type of soil had no significant effect on that of S. multijuga. Conclusions These results show that soil characteristics also play an important role in determining phenological patterns and growth and must be considered when analysing phenological patterns in tropical forests. |
Palavras-Chave: |
Citharexylum myrianthum; Floresta sazonal; Ritmo sazonal; Senna multijuga. |
Thesagro: |
Crescimento; Diâmetro; Nutriente; Solo. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02164naa a2200253 a 4500 001 1924782 005 2015-02-20 008 2012 bl uuuu u00u1 u #d 100 1 $aCARDOSO, F. C. 245 $aStem growth and phenology of two tropical trees in contrasting soil conditions.$h[electronic resource] 260 $c2012 520 $aBackground and aims Phenological variations in tropical forests are usually explained by climate. Nevertheless, considering that soil water availability and nutrient content also influence plant water status and metabolism, soil conditions may also be important in the regulation of plant reproductive and vegetative activities over time.We investigated whether phenological patterns and stem growth differ in trees growing in two types of soil that display contrasting water and nutrient availability, namely, Gleysol (moist and nutrient-poor) and Cambisol (drier and nutrient rich). Methods Phenological observations (flushing, leaf fall, flowering and fruiting) and stem diameter growth were recorded for 120 trees fitted with fixed dendrometer bands, at 15 days intervals, for 1 year. Two species of contrasting deciduousness were investigated: Senna multijuga (semi-deciduous) and Citharexylum myrianthum (deciduous). Results Both species were seasonal in all phenophases, regardless of soil type. However, frequency, mean date and intensity of phenophases varied according to soil type. Girth increment of C. myrianthum was four times greater in Cambisol than in Gleysol, whereas the type of soil had no significant effect on that of S. multijuga. Conclusions These results show that soil characteristics also play an important role in determining phenological patterns and growth and must be considered when analysing phenological patterns in tropical forests. 650 $aCrescimento 650 $aDiâmetro 650 $aNutriente 650 $aSolo 653 $aCitharexylum myrianthum 653 $aFloresta sazonal 653 $aRitmo sazonal 653 $aSenna multijuga 700 1 $aMARQUES, R. 700 1 $aBOTOSSO, P. C. 700 1 $aMARQUES, M. C. M. 773 $tPlant and Soil$gn. 354, p. 269-281, 2012.
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
28/08/2018 |
Data da última atualização: |
12/09/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
TOSCANO, J. H. B.; LOPES, L. G.; GIRALDELO, L. A.; SILVA, M. H. da; OKINO, C. H.; CHAGAS, A. C. de S. |
Afiliação: |
João Henrique Barbosa Toscano, UNESP; Louyse Gabrielli Lopes, UNICEP; Luciana Aparecida Giraldelo, UNICEP; Matheus Henrique da Silva, UNICEP; CINTIA HIROMI OKINO, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
Título: |
Identification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Molecular Biology Reports, v.45, n.5, p.1253-1262, out. 2018. |
Idioma: |
Inglês |
Conteúdo: |
Due to the great economic impact of Haemonchus contortus on sheep farming, there is an increasing number of studies addressing host resistance against this nematode, including identification of directly related immune mechanisms. In this context, relative gene expression by RT-qPCR have been largely used, due to its rapidity, high sensitivity, specificity, and reproducibility. Although, appropriate reference gene selection is crucial for accurate interpretation of results. In this study, five reference genes (GAPDH, G6PDH, YWHAZ, ACTB, and B2M) were tested for expression stability in abomasum (fundic and pyloric regions) and abomasal lymph nodes of Morada Nova sheep classified as resistant (n = 5) or susceptible (n = 5) to H. contortus infection in a flock of 151 animals. GAPDH combined with YWHAZ were selected as reference genes for abomasal fundic region and abomasal lymph nodes, whereas YWHAZ was the most stable gene for abomasal pyloric region. These genes presented the lowest intra- and inter-group variations and, consequently, highest stability. In contrast, expression of G6PDH was the least stable in all tissues. The impact of reference gene selection was demonstrated by relative quantification of a pro-inflammatory cytokine (TNFα) in abomasal fundic region. Significant differences in TNFα expression levels between resistant and susceptible groups were only observed when the most stable genes (GAPDH combined with YWHAZ) or GAPDH were used as reference genes, whereas no significant differences were observed when other tested reference genes were used. It was demonstrated that normalization of expression data using inappropriate reference genes may significantly influence interpretation results. MenosDue to the great economic impact of Haemonchus contortus on sheep farming, there is an increasing number of studies addressing host resistance against this nematode, including identification of directly related immune mechanisms. In this context, relative gene expression by RT-qPCR have been largely used, due to its rapidity, high sensitivity, specificity, and reproducibility. Although, appropriate reference gene selection is crucial for accurate interpretation of results. In this study, five reference genes (GAPDH, G6PDH, YWHAZ, ACTB, and B2M) were tested for expression stability in abomasum (fundic and pyloric regions) and abomasal lymph nodes of Morada Nova sheep classified as resistant (n = 5) or susceptible (n = 5) to H. contortus infection in a flock of 151 animals. GAPDH combined with YWHAZ were selected as reference genes for abomasal fundic region and abomasal lymph nodes, whereas YWHAZ was the most stable gene for abomasal pyloric region. These genes presented the lowest intra- and inter-group variations and, consequently, highest stability. In contrast, expression of G6PDH was the least stable in all tissues. The impact of reference gene selection was demonstrated by relative quantification of a pro-inflammatory cytokine (TNFα) in abomasal fundic region. Significant differences in TNFα expression levels between resistant and susceptible groups were only observed when the most stable genes (GAPDH combined with YWHAZ) or GAPDH were used as reference genes,... Mostrar Tudo |
Palavras-Chave: |
Genes de referência. |
Thesagro: |
Haemonchus Contortus; Ovino. |
Thesaurus NAL: |
Sheep. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02463naa a2200229 a 4500 001 2094611 005 2018-09-12 008 2018 bl uuuu u00u1 u #d 100 1 $aTOSCANO, J. H. B. 245 $aIdentification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus.$h[electronic resource] 260 $c2018 520 $aDue to the great economic impact of Haemonchus contortus on sheep farming, there is an increasing number of studies addressing host resistance against this nematode, including identification of directly related immune mechanisms. In this context, relative gene expression by RT-qPCR have been largely used, due to its rapidity, high sensitivity, specificity, and reproducibility. Although, appropriate reference gene selection is crucial for accurate interpretation of results. In this study, five reference genes (GAPDH, G6PDH, YWHAZ, ACTB, and B2M) were tested for expression stability in abomasum (fundic and pyloric regions) and abomasal lymph nodes of Morada Nova sheep classified as resistant (n = 5) or susceptible (n = 5) to H. contortus infection in a flock of 151 animals. GAPDH combined with YWHAZ were selected as reference genes for abomasal fundic region and abomasal lymph nodes, whereas YWHAZ was the most stable gene for abomasal pyloric region. These genes presented the lowest intra- and inter-group variations and, consequently, highest stability. In contrast, expression of G6PDH was the least stable in all tissues. The impact of reference gene selection was demonstrated by relative quantification of a pro-inflammatory cytokine (TNFα) in abomasal fundic region. Significant differences in TNFα expression levels between resistant and susceptible groups were only observed when the most stable genes (GAPDH combined with YWHAZ) or GAPDH were used as reference genes, whereas no significant differences were observed when other tested reference genes were used. It was demonstrated that normalization of expression data using inappropriate reference genes may significantly influence interpretation results. 650 $aSheep 650 $aHaemonchus Contortus 650 $aOvino 653 $aGenes de referência 700 1 $aLOPES, L. G. 700 1 $aGIRALDELO, L. A. 700 1 $aSILVA, M. H. da 700 1 $aOKINO, C. H. 700 1 $aCHAGAS, A. C. de S. 773 $tMolecular Biology Reports$gv.45, n.5, p.1253-1262, out. 2018.
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