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Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
22/11/2001 |
Data da última atualização: |
31/05/2007 |
Autoria: |
SIMÕES-ARAÚJO-ARAUJO, J. L.; RODRIGUES, R. L.; GERHARDT, L. B. de A.; MONDEGO, J. M. C.; ALVES-FERREIRA, M.; RUMJANEK, N. G.; MARGIS-PINHEIRO, M. |
Título: |
Identification of differentially expressed genes by cDNA-AFLP technique during heat stress in cowpea nodules. |
Ano de publicação: |
2002 |
Fonte/Imprenta: |
FEBS Letters, Netherlands, v. 515, p. 44-50, 2002. |
Idioma: |
Inglês |
Conteúdo: |
Legume nodules formed by diazotrophic microorganisrns are active sites for biological nitrogen fixation (BNF). In tropical regions, a significant part of N supply for soybean, peanut and bean crops is derived from BNF , which is nevertheless often limited by high temperature stress. In contrast, cowpea nodules are very resistant to high temperatures. To understand the molecular bases of thermotolerance during BNF under heat stress, we have used cDNA-amplified fragment length polymorphism experiments to identify differentially expressed transcripts from cowpea nodules subjected to heat shock treatment. The expression profiles obtained showed approximately 600 bands, 55 up-regulated and nine corresponding to genes repressed by heat stress. Twenty transcript-derived fragments were isolated, cloned and sequenced. The Vigna unguiculata nodule and stress response transcripts present similarities to those that encode low molecular weight heat shock proteins, wound-induced proteins, disease resistance protein, and xylan endohydrolase isoenzyme, as well as different housekeeping genes. The differential expression of 15 genes was confirmed by using Northern blot or reverse Northern hybridization experiments. @ 2002 Federation of European Biochemical Societies. Published by Elsevier Science B. V. AJI rights reserved.
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Palavras-Chave: |
Biological nitrogen fixation; BNF; Caupi; FBN; Fixação bioloógica de nitrogênio. |
Thesagro: |
Vigna Unguiculata. |
Thesaurus Nal: |
cowpeas. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02152naa a2200277 a 4500 001 1598731 005 2007-05-31 008 2002 bl --- 0-- u #d 100 1 $aSIMÕES-ARAÚJO-ARAUJO, J. L. 245 $aIdentification of differentially expressed genes by cDNA-AFLP technique during heat stress in cowpea nodules. 260 $c2002 520 $aLegume nodules formed by diazotrophic microorganisrns are active sites for biological nitrogen fixation (BNF). In tropical regions, a significant part of N supply for soybean, peanut and bean crops is derived from BNF , which is nevertheless often limited by high temperature stress. In contrast, cowpea nodules are very resistant to high temperatures. To understand the molecular bases of thermotolerance during BNF under heat stress, we have used cDNA-amplified fragment length polymorphism experiments to identify differentially expressed transcripts from cowpea nodules subjected to heat shock treatment. The expression profiles obtained showed approximately 600 bands, 55 up-regulated and nine corresponding to genes repressed by heat stress. Twenty transcript-derived fragments were isolated, cloned and sequenced. The Vigna unguiculata nodule and stress response transcripts present similarities to those that encode low molecular weight heat shock proteins, wound-induced proteins, disease resistance protein, and xylan endohydrolase isoenzyme, as well as different housekeeping genes. The differential expression of 15 genes was confirmed by using Northern blot or reverse Northern hybridization experiments. @ 2002 Federation of European Biochemical Societies. Published by Elsevier Science B. V. AJI rights reserved. 650 $acowpeas 650 $aVigna Unguiculata 653 $aBiological nitrogen fixation 653 $aBNF 653 $aCaupi 653 $aFBN 653 $aFixação bioloógica de nitrogênio 700 1 $aRODRIGUES, R. L. 700 1 $aGERHARDT, L. B. de A. 700 1 $aMONDEGO, J. M. C. 700 1 $aALVES-FERREIRA, M. 700 1 $aRUMJANEK, N. G. 700 1 $aMARGIS-PINHEIRO, M. 773 $tFEBS Letters, Netherlands$gv. 515, p. 44-50, 2002.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Registro Completo
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
21/01/2014 |
Data da última atualização: |
16/05/2018 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
OLIVEIRA JUNIOR, R. C. de; SILVA FILHO, E. P.; RODRIGUES, T. E.; SANTOS, P. L. dos. |
Afiliação: |
RAIMUNDO COSME DE OLIVEIRA JUNIOR, CPATU; ELIOMAR PEREIRA SILVA FILHO, UFRO; TARCÍSIO EWERTON RODRIGUES, CPATU; PAULO LACERDA DOS SANTOS, CPATU. |
Título: |
Caracterização e mapeamento dos solos do campo experimental de Porto Velho, CPAF-RO. |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 26., 1997, Rio de Janeiro. Informação de solo na globalização do conhecimento sobre o uso das terras: resumos. Rio de Janeiro: SBCS, 1997. |
Páginas: |
p. 339. |
Idioma: |
Português |
Palavras-Chave: |
Mapeamento; Porto velho; Rondônia. |
Thesagro: |
Solo. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/176946/1/Mapeamento-dos-solos.pdf
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Marc: |
LEADER 00699nam a2200193 a 4500 001 1976849 005 2018-05-16 008 1997 bl uuuu u00u1 u #d 100 1 $aOLIVEIRA JUNIOR, R. C. de 245 $aCaracterização e mapeamento dos solos do campo experimental de Porto Velho, CPAF-RO. 260 $aIn: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 26., 1997, Rio de Janeiro. Informação de solo na globalização do conhecimento sobre o uso das terras: resumos. Rio de Janeiro: SBCS$c1997 300 $ap. 339. 650 $aSolo 653 $aMapeamento 653 $aPorto velho 653 $aRondônia 700 1 $aSILVA FILHO, E. P. 700 1 $aRODRIGUES, T. E. 700 1 $aSANTOS, P. L. dos
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