Data/hora: 02/06/2024 01:18:32

Biblioteca(s): | Embrapa Agrobiologia.
Data corrente: | 22/11/2001
Data da última atualização: | 31/05/2007
Autoria: | SIMÕES-ARAÚJO-ARAUJO, J. L.; RODRIGUES, R. L.; GERHARDT, L. B. de A.; MONDEGO, J. M. C.; ALVES-FERREIRA, M.; RUMJANEK, N. G.; MARGIS-PINHEIRO, M.
Título: | Identification of differentially expressed genes by cDNA-AFLP technique during heat stress in cowpea nodules.
Ano de publicação: | 2002
Fonte/Imprenta: | FEBS Letters, Netherlands, v. 515, p. 44-50, 2002.
Idioma: | Inglês
Conteúdo: | Legume nodules formed by diazotrophic microorganisrns are active sites for biological nitrogen fixation (BNF). In tropical regions, a significant part of N supply for soybean, peanut and bean crops is derived from BNF , which is nevertheless often limited by high temperature stress. In contrast, cowpea nodules are very resistant to high temperatures. To understand the molecular bases of thermotolerance during BNF under heat stress, we have used cDNA-amplified fragment length polymorphism experiments to identify differentially expressed transcripts from cowpea nodules subjected to heat shock treatment. The expression profiles obtained showed approximately 600 bands, 55 up-regulated and nine corresponding to genes repressed by heat stress. Twenty transcript-derived fragments were isolated, cloned and sequenced. The Vigna unguiculata nodule and stress response transcripts present similarities to those that encode low molecular weight heat shock proteins, wound-induced proteins, disease resistance protein, and xylan endohydrolase isoenzyme, as well as different housekeeping genes. The differential expression of 15 genes was confirmed by using Northern blot or reverse Northern hybridization experiments. @ 2002 Federation of European Biochemical Societies. Published by Elsevier Science B. V. AJI rights reserved.
Palavras-Chave: | Biological nitrogen fixation; BNF; Caupi; FBN; Fixação bioloógica de nitrogênio.
Thesagro: | Vigna Unguiculata.
Thesaurus Nal: | cowpeas.
Categoria do assunto: | --
Registro original: | Embrapa Agrobiologia (CNPAB)
Exemplares: | CNPAB - (2428-1) - UPC - SP - -- -  -  - 0022585 - DIS