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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
28/08/2014 |
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Data da última atualização: |
02/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PERINI, P.; PASQUALI, G.; MARGIS-PINHEIRO, M.; OLIVEIRA, P. R. D. de; REVERS, L. F. |
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Afiliação: |
Pâmela Perini; Giancarlo Pasquali; Márcia Margis-Pinheiro; PAULO RICARDO DIAS DE OLIVEIRA, CNPUV; LUIS FERNANDO REVERS, CNPUV. |
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Título: |
Reference genes for transcriptional analysis of flowering and fruit ripening stages in apple (Malus 3 domestica Borkh.). |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Molecular Breeding, mar. 2014. |
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Idioma: |
Inglês |
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Notas: |
DOI 10.1007/s11032-014-0078-3 |
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Conteúdo: |
Apple (Malus 9 domestica Borkh.) is the most important deciduous tree fruit crop grown around the world. Comparisons of gene expression profiles from different tissues, conditions or cultivars are valuable scientific tools to better understand the gene expression changes behind important silvicultural and nutritional traits. However, the accuracy of techniques employed to access gene expression is dependent on the evaluation of stable reference genes for data normalization to avoid statistical significance undue or incorrect conclusions. The objective of this work was to select the best genes to be used as references for gene expression studies in apple trees by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Vegetative and reproductive tissues of the apple ??Gala?? cultivar were evaluated during their seasonal cycle of growth and dormancy. The expression of 23 traditional housekeeping genes or genes suggested as constitutive by microarray data was investigated. Tested combinations of primers allowed the specific amplification and the generation of suitable efficiency curves for gene expression studies by RT-qPCR. Gene stability was determined by two different statistical descriptors, geNorm and Norm-Finder. The known variable PAL gene expression was used to validate selected normalizers. Results obtained allowed us to conclude that MDH, SAND, THFS, TMp1 and WD40 are the best reference genes to accurately normalize the relative transcript abundances using RT-qPCR in various tissues of apple. MenosApple (Malus 9 domestica Borkh.) is the most important deciduous tree fruit crop grown around the world. Comparisons of gene expression profiles from different tissues, conditions or cultivars are valuable scientific tools to better understand the gene expression changes behind important silvicultural and nutritional traits. However, the accuracy of techniques employed to access gene expression is dependent on the evaluation of stable reference genes for data normalization to avoid statistical significance undue or incorrect conclusions. The objective of this work was to select the best genes to be used as references for gene expression studies in apple trees by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Vegetative and reproductive tissues of the apple ??Gala?? cultivar were evaluated during their seasonal cycle of growth and dormancy. The expression of 23 traditional housekeeping genes or genes suggested as constitutive by microarray data was investigated. Tested combinations of primers allowed the specific amplification and the generation of suitable efficiency curves for gene expression studies by RT-qPCR. Gene stability was determined by two different statistical descriptors, geNorm and Norm-Finder. The known variable PAL gene expression was used to validate selected normalizers. Results obtained allowed us to conclude that MDH, SAND, THFS, TMp1 and WD40 are the best reference genes to accurately normalize the relative transcript abundances u... Mostrar Tudo |
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Palavras-Chave: |
Expressão genética; Gala; Genes de referencia; RT-qPCR. |
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Thesagro: |
Genetica vegetal; Maçã. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/107436/1/Perini2014-Reference-Genes-Apple.pdf
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Marc: |
LEADER 02287naa a2200253 a 4500
001 1993623
005 2019-04-02
008 2014 bl uuuu u00u1 u #d
100 1 $aPERINI, P.
245 $aReference genes for transcriptional analysis of flowering and fruit ripening stages in apple (Malus 3 domestica Borkh.).$h[electronic resource]
260 $c2014
500 $aDOI 10.1007/s11032-014-0078-3
520 $aApple (Malus 9 domestica Borkh.) is the most important deciduous tree fruit crop grown around the world. Comparisons of gene expression profiles from different tissues, conditions or cultivars are valuable scientific tools to better understand the gene expression changes behind important silvicultural and nutritional traits. However, the accuracy of techniques employed to access gene expression is dependent on the evaluation of stable reference genes for data normalization to avoid statistical significance undue or incorrect conclusions. The objective of this work was to select the best genes to be used as references for gene expression studies in apple trees by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Vegetative and reproductive tissues of the apple ??Gala?? cultivar were evaluated during their seasonal cycle of growth and dormancy. The expression of 23 traditional housekeeping genes or genes suggested as constitutive by microarray data was investigated. Tested combinations of primers allowed the specific amplification and the generation of suitable efficiency curves for gene expression studies by RT-qPCR. Gene stability was determined by two different statistical descriptors, geNorm and Norm-Finder. The known variable PAL gene expression was used to validate selected normalizers. Results obtained allowed us to conclude that MDH, SAND, THFS, TMp1 and WD40 are the best reference genes to accurately normalize the relative transcript abundances using RT-qPCR in various tissues of apple.
650 $aGenetica vegetal
650 $aMaçã
653 $aExpressão genética
653 $aGala
653 $aGenes de referencia
653 $aRT-qPCR
700 1 $aPASQUALI, G.
700 1 $aMARGIS-PINHEIRO, M.
700 1 $aOLIVEIRA, P. R. D. de
700 1 $aREVERS, L. F.
773 $tMolecular Breeding, mar. 2014.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Amapá. |
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Data corrente: |
13/03/2000 |
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Data da última atualização: |
05/10/2022 |
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Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
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Autoria: |
MOCHIUTTI, S.; MELEM JUNIOR, N. J.; FARIAS NETO, J. T. de; QUEIROZ, J. A. L. de. |
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Afiliação: |
SILAS MOCHIUTTI, CPAF-AP; NAGIB JORGE MELEM JUNIOR, CPAF-AP; JOAO TOME DE FARIAS NETO, CPAF-AP; JOSE ANTONIO LEITE DE QUEIROZ, CPAF-AP. |
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Título: |
Taxi-branco (Sclerolobium paniculatum Vogel): leguminosa arborea para recuperação de áreas degradadas e abandonadas pela agricultura migratoria. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Macapá: Embrapa Amapá, 1999. |
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Páginas: |
5 p. |
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Série: |
(Embrapa Amapá. Comunicado técnico, 28). |
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Idioma: |
Português |
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Conteúdo: |
No Estado do Amapá, como em toda a região amazonica, a agricultura caracteriza-se pela derruba e queima da floresta, com o plantio de culturas de subsistência, principalmente a mandioca, por dois ou três anos consecutivos e posterior abandono da área. Neste sistema, há uma redução da produção dos cultivos a cada ano, causada pela diminuição da capacidade produtiva dos solos, obrigando o agicultor a realizar desmatamentos de novas áreas. |
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Palavras-Chave: |
Amapa; Arboreal leguminosa; Área degradada; Brasil; Código Florestal; Degradacao; Leguminosa arborea; Macapa; Reclamation; Recuperação; Táxi-branco. |
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Thesagro: |
Agricultura; Árvore; Desmatamento; Deterioração do Solo; Floresta Tropical Úmida; Recuperação do Solo; Sclerolobium Paniculatum; Solo; Taxi Branco. |
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Thesaurus NAL: |
agriculture; deforestation; degradation; environmental degradation; soil; tropical rain forests. |
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Categoria do assunto: |
--
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/35266/1/taxi-branco-CT.pdf
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Marc: |
LEADER 01818nam a2200481 a 4500
001 1344125
005 2022-10-05
008 1999 bl uuuu u0uu1 u #d
100 1 $aMOCHIUTTI, S.
245 $aTaxi-branco (Sclerolobium paniculatum Vogel)$bleguminosa arborea para recuperação de áreas degradadas e abandonadas pela agricultura migratoria.
260 $aMacapá: Embrapa Amapá$c1999
300 $a5 p.
490 $a(Embrapa Amapá. Comunicado técnico, 28).
520 $aNo Estado do Amapá, como em toda a região amazonica, a agricultura caracteriza-se pela derruba e queima da floresta, com o plantio de culturas de subsistência, principalmente a mandioca, por dois ou três anos consecutivos e posterior abandono da área. Neste sistema, há uma redução da produção dos cultivos a cada ano, causada pela diminuição da capacidade produtiva dos solos, obrigando o agicultor a realizar desmatamentos de novas áreas.
650 $aagriculture
650 $adeforestation
650 $adegradation
650 $aenvironmental degradation
650 $asoil
650 $atropical rain forests
650 $aAgricultura
650 $aÁrvore
650 $aDesmatamento
650 $aDeterioração do Solo
650 $aFloresta Tropical Úmida
650 $aRecuperação do Solo
650 $aSclerolobium Paniculatum
650 $aSolo
650 $aTaxi Branco
653 $aAmapa
653 $aArboreal leguminosa
653 $aÁrea degradada
653 $aBrasil
653 $aCódigo Florestal
653 $aDegradacao
653 $aLeguminosa arborea
653 $aMacapa
653 $aReclamation
653 $aRecuperação
653 $aTáxi-branco
700 1 $aMELEM JUNIOR, N. J.
700 1 $aFARIAS NETO, J. T. de
700 1 $aQUEIROZ, J. A. L. de
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Registro original: |
Embrapa Amapá (CPAF-AP) |
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