Data/hora: 11/06/2024 03:12:52

Biblioteca(s): | Embrapa Soja.
Data corrente: | 19/10/2010
Data da última atualização: | 25/07/2017
Autoria: | KULCHESKI, F. R.; MARCELINO-GUIMARAES, F. C.; NEPOMUCENO, A. L.; ABDELNOOR, R. V.; MARGIS, R.
Afiliação: | FRANCELI RODRIGUES KULCHESKI, UFRGS; FRANCISMAR CORREA MARCELINO, CNPSO; ALEXANDRE LIMA NEPOMUCENO, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; ROGÉRIO MARGIS, UFRGS.
Título: | The use of microRNAs as reference genes for quantitative polymerase chain reaction in soybean.
Ano de publicação: | 2010
Fonte/Imprenta: | Analytical Biochemistry, Amsterdam, v. 406, n. 2, p. 185-192, nov. 2010.
DOI: | 10.1016/j.ab.2010.07.020
Idioma: | Português
Conteúdo: | Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is a robust and widely applied technique used to investigate gene expression. However, for correct analysis and interpretation of results, the choice of a suitable gene to use as an internal control is a crucial factor. These genes, such as housekeeping genes, should have a constant expression level in different tissues and across different conditions. The advances in genome sequencing have provided high-throughput gene expression analysis and have contributed to the identification of new genes, including microRNAs (miRNAs). The miRNAs are fundamental regulatory genes of eukaryotic genomes, acting on several biological functions. In this study, miRNA expression stability was investigated in different soybean tissues and genotypes as well as after abiotic or biotic stress treatments. The present study represents the first investigation into the suitability of miRNAs as housekeeping genes in plants. The transcript stability of 10 miRNAs was compared to those of six previously reported housekeeping genes for the soybean. In this study, we provide evidence that the expression stabilities of miR156b and miR1520d were the highest across the soybean experiments. Furthermore, these miRNAs genes were more stable than the most commonly protein-coding genes used in soybean gene expression studies involving RT-qPCR.
Palavras-Chave: | Reação em cadeia da polimerase.
Thesagro: | Gene; RNA; Soja.
Thesaurus Nal: | Genes; MicroRNA; Quantitative polymerase chain reaction; Soybeans.
Categoria do assunto: | G Melhoramento Genético
Registro original: | Embrapa Soja (CNPSO)
Exemplares: | CNPSO - (31392-1) - UPC - AP - PP - 11873 -  - 11873 - DIS