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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
03/11/2014 |
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Data da última atualização: |
05/04/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BARROS-CARVALHO, G. A.; PASCHOAL, A. R.; MARCELINO-GUIMARÃES, F. C.; HUNGRIA, M. |
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Afiliação: |
GESIELE ALMEIDA BARROS DE CARVALHO, CNPq - bolsista; ALEXANDRE ROSSI PASCHOAL, UTFPR; FRANCISMAR CORRÊA MARCELINO-GUIMARÃES, CNPSO; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Prediction of potential novel microRNAs in soybean when in symbiosis. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 13, n. 4, p. 8519-8529, 2014. |
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ISSN: |
1676-5680 |
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DOI: |
10.4238/2014.October.20.28 |
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Idioma: |
Inglês |
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Conteúdo: |
MicroRNAs (miRNAs) are small molecules, noncoding proteins that are involved in many biological processes, especially in plants; among these processes is nodulation in the legume. Biological nitrogen fixation is a key process, with critical importance to the soybean crop. This study aimed to identify the potential of novel miRNAs to act during the root nodulation process. We utilized a set of transcripts that were differentially expressed in soybean roots 10 days after inoculation with Bradyrhizobium japonicum, which were obtained in a previous study, and performed a set of computational analyses that led us to select new miRNAs potentially involved in nodulation. Among these analyses, the set of transcripts were submitted to an in silico annotation of noncoding RNAs, including a search of similarity against miRNA public databases, ab initio tools for miRNA identification, structural search against miRNA families, prediction of the secondary structure of miRNA precursors, and prediction of the sequences of mature miRNAs. Subsequently, we applied filter procedures based on miRNA selections described in the literature (e.g., free energy value). In the next step, a manual curation inspection of the annotation was performed and the top candidates were selected and used for prediction of potential target genes, which were later checked manually in the database of the soybean genome. This prediction led us to the identification of 9 potential new miRNAs; among these, 4 were conserved in other plants. Moreover, we predicted their target genes might play important roles in the regulation of nodulation. MenosMicroRNAs (miRNAs) are small molecules, noncoding proteins that are involved in many biological processes, especially in plants; among these processes is nodulation in the legume. Biological nitrogen fixation is a key process, with critical importance to the soybean crop. This study aimed to identify the potential of novel miRNAs to act during the root nodulation process. We utilized a set of transcripts that were differentially expressed in soybean roots 10 days after inoculation with Bradyrhizobium japonicum, which were obtained in a previous study, and performed a set of computational analyses that led us to select new miRNAs potentially involved in nodulation. Among these analyses, the set of transcripts were submitted to an in silico annotation of noncoding RNAs, including a search of similarity against miRNA public databases, ab initio tools for miRNA identification, structural search against miRNA families, prediction of the secondary structure of miRNA precursors, and prediction of the sequences of mature miRNAs. Subsequently, we applied filter procedures based on miRNA selections described in the literature (e.g., free energy value). In the next step, a manual curation inspection of the annotation was performed and the top candidates were selected and used for prediction of potential target genes, which were later checked manually in the database of the soybean genome. This prediction led us to the identification of 9 potential new miRNAs; among these, 4 were conser... Mostrar Tudo |
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Thesagro: |
Soja. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/110912/1/Prediction-of-potential-novel-microRNAs-in-soybean-when-in-symbiosis.pdf
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Marc: |
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001 1999050
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008 2014 bl uuuu u00u1 u #d
022 $a1676-5680
024 7 $a10.4238/2014.October.20.28$2DOI
100 1 $aBARROS-CARVALHO, G. A.
245 $aPrediction of potential novel microRNAs in soybean when in symbiosis.$h[electronic resource]
260 $c2014
520 $aMicroRNAs (miRNAs) are small molecules, noncoding proteins that are involved in many biological processes, especially in plants; among these processes is nodulation in the legume. Biological nitrogen fixation is a key process, with critical importance to the soybean crop. This study aimed to identify the potential of novel miRNAs to act during the root nodulation process. We utilized a set of transcripts that were differentially expressed in soybean roots 10 days after inoculation with Bradyrhizobium japonicum, which were obtained in a previous study, and performed a set of computational analyses that led us to select new miRNAs potentially involved in nodulation. Among these analyses, the set of transcripts were submitted to an in silico annotation of noncoding RNAs, including a search of similarity against miRNA public databases, ab initio tools for miRNA identification, structural search against miRNA families, prediction of the secondary structure of miRNA precursors, and prediction of the sequences of mature miRNAs. Subsequently, we applied filter procedures based on miRNA selections described in the literature (e.g., free energy value). In the next step, a manual curation inspection of the annotation was performed and the top candidates were selected and used for prediction of potential target genes, which were later checked manually in the database of the soybean genome. This prediction led us to the identification of 9 potential new miRNAs; among these, 4 were conserved in other plants. Moreover, we predicted their target genes might play important roles in the regulation of nodulation.
650 $aSoja
700 1 $aPASCHOAL, A. R.
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aHUNGRIA, M.
773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 13, n. 4, p. 8519-8529, 2014.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 188 | |
| 7. |  | CARDOSO, R.; CAITAR, V. L.; FERREIRA, E. C.; MARCELINO-GUIMARÃES, F. C.; ABDELNOOR, R. V. Associative mapping for bacterial pustule in soybean (Mapeamento associativo para pústula bacteriana em soja) in: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 51., 2019, Recife. Anais... Brasília, DF: SBF, 2019. p. 156.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 12. | | BORTOLAN, S.; BARCELLOS, F. G.; MARCELINO-GUIMARÃES, F. C.; HUNGRIA, M. Expressão dos genes nodC, nodW e nopP em Bradyrhizobium japonicum estirpe CPAC 15 avaliada por RT-qPCR. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 11, p. 1491-1498, nov. 2009 Título em inglês: Expression of nodC, nodW and nopP genes in Bradyrhizobiumjaponicum CPAC 15 strain evaluated by RT-qPCR.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 13. | | OLIVEIRA, L. R.; RODRIGUES, E. P.; MARCELINO-GUIMARÃES, F. C.; OLIVEIRA, A. L. M.; HUNGRIA, M. Fast induction of biosynthetic polysaccharide genes lpxA, lpxE, and rkpI of Rhizobium sp. strain PRF 81 by common bean seed exudates is indicative of a key role in symbiosis. Functional & Integrative Genomics, v. 13, n. 2, p. 275-283, Jun. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 17. | | NOMURA, R. B. G.; LOPES-CAITAR, V. S.; HISHINUMA-SILVA, S. M.; MARCELINO-GUIMARÃES, F. C. Novos aspectos da interação Pratylenchus brachyurus-soja baseados na predição das proteínas-alvo no hospedeiro. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 16., 2021, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2021. p. 86-93. (Embrapa Soja. Documentos, 440). Regina Maria Villas Bôas de Campos Leite, Kelly Catharin, editoras técnicas. Acesso aberto.
Open access.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 20. | | RODRIGUES, H. P.; SILVA, D. C. G. da; FERREIRA, E. G. C.; MARCELINO-GUIMARÃES, F. C. Otimização de ensaios de PCR Multiplex para a amplificação de alvos de interesse para o melhoramento genético da Soja. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 15., 2020, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2020. 244 p. (Embrapa Soja. Documentos, 429). p. 202-210 (Embrapa Soja. Documentos, 429). Artigo de acesso aberto.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 188 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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