Data/hora: 23/09/2024 02:50:27

Biblioteca(s): 	Embrapa Soja.	
Data corrente: 	19/03/2010	
Data da última atualização: 	19/01/2017	
Autoria: 	OLIVEIRA, L. R.; MARCELINO, F. C.; BARCELLOS, F. G.; RODRIGUES, E. P.; MEGIAS, M.; HUNGRIA, M.	
Afiliação: 	LUCIANA RUANO OLIVEIRA, UEL - CNPq; FRANCISMAR CORREA MARCELINO, CNPSo; FERNANDO GOMES BARCELLOS, CNPSo; ELISETE PAINS RODRIGUES, CNPSo - CNPq; MANUEL MEGIAS, Universidad de Sevilha; MARIANGELA HUNGRIA DA CUNHA, CNPSo.	
Título: 	The nodC, nodG, and glgX genes of Rhizobium tropici strain PRF 81.	
Ano de publicação: 	2010	
Fonte/Imprenta: 	Functional & Integrative Genomics, Heidelberg, v. 10, n. 3, p. 425-431, Aug. 2010.	
DOI: 	DOI 10.1007/s10142-009-0151-x	
Idioma: 	Inglês	
Conteúdo: 	Rhizobium tropici is a diazotrophic microsymbiont of common bean (Phaseolus vulgaris L.) that encompasses important but still poorly studied tropical strains, and a recent significant contribution to the knowledge of the species was the publication of a genomic draft of strain PRF 81, which revealed several novel genes [Pinto et al. Funct Int Gen 9:263?270, 2009]. In this study, we investigated the transcription of nodC, nodG, and glgX genes, located in the nod operon of PRF 81 strain, by reverse-transcription quantitative PCR. All three genes showed low levels of transcription when the cells were grown until exponential growth phase in the presence of common-bean-seed exudates or of the root nod-gene inducer naringenin. However, when cells at the exponential phase of growth were incubated with seed exudates, transcription occurred after only 5 min, and nodC, nodG, and glgX were transcribed 121.97-, 14.86-, and 50.29-fold more than the control, respectively, followed by a rapid decrease in gene transcription. Much lower levels of transcription were observed in the presence of naringenin; furthermore, maximum transcription required 8 h of incubation for all three genes. In light of these results, the mechanisms of induction of the nodulation genes by flavonoids are discussed.	
Palavras-Chave: 	Reverse transcription quantitative-PCR.	
Thesagro: 	Feijao; Fixacao de nitrogenio; Gene; Phaseolus Vulgaris; Simbiose.	
Thesaurus Nal: 	Beans; Genes; Nitrogen fixation; Rhizobium tropici; Symbiosis.	
Categoria do assunto: 	G Melhoramento Genético	
Registro original: 	Embrapa Soja (CNPSO)	
Exemplares: 	CNPSO - (30608-1) - UPC - AP - PP - 11785 -  - 11785 - DIS