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Registro Completo |
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
24/05/2022 |
Data da última atualização: |
23/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MARGARIDO, A.; MANZINE, L. R.; ARAÚJO-MOREIRA, F. M.; GONÇALVES, R. V.; HERRMANN JUNIOR, P. S. de P. |
Afiliação: |
PAULO SERGIO DE P HERRMANN JUNIOR, CNPDIA. |
Título: |
Specific detection of short-chain alcohols, with the development of an enzyme-coated microcantilever-based biosensor. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Brazilian Journal of Physics, v. 52, 112, 2022. |
Páginas: |
14 p. |
DOI: |
10.1007/s13538-022-01111-w |
Idioma: |
Inglês |
Conteúdo: |
This work describes the development and evaluation of a biosensor designed for enzymatic detection of short-chain alcohols, using an X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The biorecognition element, alcohol dehydrogenase, was immobilized on self-assembled monolayers deposited on top of silicon nitride microcantilevers. Biosensor assays provided detection of short-chain alcohols and evaluation of the infuence of a biological element immobilization on the analytical performance of the device. The self-assembly process was performed by surface activation using 3-aminopropyltriethoxysilane, followed by glutaraldehyde and biomolecule binding. XPS and AFM were used to verify surface oxidation and reagent binding. The biosensor showed a response time shorter than 1 s, a total renovation of the bioactive layer after 10 min, a sensibility from 0.03 to 1.2 mL/L, and a lifetime of 22 days. Its selectivity was analyzed through exposure to pure and mixed volatile solvents. Sensor sensibility was higher in the presence of short-chain alcohols family (methanol, ethanol, and propanol) ranging from 0.45 to 0.85 kHz and practically null involving other polar or nonpolar solvents. The biosensor showed less susceptibility to humidity and temperature variations, presenting a high-quality factor, a faster response time, selectivity, sensitivity, and durability. |
Palavras-Chave: |
Biosensor; Gas detector; Microcantilever; Short-chain alcohols; XPS. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1143406/1/P-Specific-Detection-of-Short8209Chain-Alcohols-with-the-Development.pdf
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Marc: |
LEADER 02171naa a2200253 a 4500 001 2143406 005 2024-01-23 008 2022 bl uuuu u00u1 u #d 024 7 $a10.1007/s13538-022-01111-w$2DOI 100 1 $aMARGARIDO, A. 245 $aSpecific detection of short-chain alcohols, with the development of an enzyme-coated microcantilever-based biosensor.$h[electronic resource] 260 $c2022 300 $a14 p. 520 $aThis work describes the development and evaluation of a biosensor designed for enzymatic detection of short-chain alcohols, using an X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The biorecognition element, alcohol dehydrogenase, was immobilized on self-assembled monolayers deposited on top of silicon nitride microcantilevers. Biosensor assays provided detection of short-chain alcohols and evaluation of the infuence of a biological element immobilization on the analytical performance of the device. The self-assembly process was performed by surface activation using 3-aminopropyltriethoxysilane, followed by glutaraldehyde and biomolecule binding. XPS and AFM were used to verify surface oxidation and reagent binding. The biosensor showed a response time shorter than 1 s, a total renovation of the bioactive layer after 10 min, a sensibility from 0.03 to 1.2 mL/L, and a lifetime of 22 days. Its selectivity was analyzed through exposure to pure and mixed volatile solvents. Sensor sensibility was higher in the presence of short-chain alcohols family (methanol, ethanol, and propanol) ranging from 0.45 to 0.85 kHz and practically null involving other polar or nonpolar solvents. The biosensor showed less susceptibility to humidity and temperature variations, presenting a high-quality factor, a faster response time, selectivity, sensitivity, and durability. 653 $aBiosensor 653 $aGas detector 653 $aMicrocantilever 653 $aShort-chain alcohols 653 $aXPS 700 1 $aMANZINE, L. R. 700 1 $aARAÚJO-MOREIRA, F. M. 700 1 $aGONÇALVES, R. V. 700 1 $aHERRMANN JUNIOR, P. S. de P. 773 $tBrazilian Journal of Physics$gv. 52, 112, 2022.
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Embrapa Instrumentação (CNPDIA) |
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Registros recuperados : 4 | |
1. | | MARGARIDO, A.; HERRMANN JUNIOR, P. S. de P.; MANZINE, L. R.; ARAUJO-MOREIRA, F. M. A selective VOC biosensor based on microcantilever functionalized with enzyme. Potential application on plant investigations. In: II Latin-American Conference on Plant Phenotyping and Phenomics for Plant Breeding, 2, 2017, São Carlos, SP. Proceedings... São Carlos: Embrapa Instrumentação, p. 53, 2017.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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4. | | COLUSSI, F.; SERPA, V.; DELABONA, P. da S.; MANZINE, L. R.; VOLTATÓDIO, M. L.; ALVES, R.; MELLO, B. L.; PEREIRA JUNIOR, N.; FARINAS, C. S.; GOLUBEV, A. M.; SANTOS, M. A. M.; POLIKARPOV, I. Purification, and biochemical and biophysical characterization of cellobiohydrolase I from trichoderma harzianum IOC 3844. Journal of microbiology and biotechnology, Kangnam-Gu, v. 21, n. 8, p. 808-817, 2011.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Instrumentação. |
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Registros recuperados : 4 | |
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