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Registro Completo |
Biblioteca(s): |
Embrapa Acre. |
Data corrente: |
11/06/2014 |
Data da última atualização: |
06/07/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA, C. C.; MANTELLO, C. C.; CAMPOS, T. de; SOUZA, L. M.; GONÇALVES, P. S.; SOUZA, A. P. |
Afiliação: |
Carla C. Silva, Universidade Estadual de Campinas (Unicamp); Camila C. Mantello, Universidade Estadual de Campinas (Unicamp); TATIANA DE CAMPOS, CPAF-AC; Livia M. Souza, Universidade Estadual de Campinas (Unicamp); Paulo S. Gonçalves, Instituto Agronômico de Campinas (IAC); Anete P. Souza, Universidade Estadual de Campinas (Unicamp). |
Título: |
Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted Functional markers for stress response. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Molecular Breeding, Berlim, v. 34, n. 3, p. 1035-1053, Oct. 2014. |
ISSN: |
1380-3743 (impresso) / 1572-9788 (on-line) |
DOI: |
10.1007/s11032-014-0095-2 |
Idioma: |
Inglês |
Conteúdo: |
Hevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consuming and expensive, but molecular markers can serve as a tool for early valuation, thus reducing time and costs. In this work, we constructed six different cDNA libraries with the aim of developing gene-targeted molecular markers for the rubber tree. A total of 8,263 reads were assembled, generating 5,025 unigenes that were analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, and two sequences were highly up-regulated by cold stress. These unigenes were scanned for microsatellite (SSR) regions and single nucleotide polymorphisms (SNPs). In total, 169 novel EST-SSR markers were developed; 138 loci were polymorphic in the rubber tree, and 98 % presented transferability to six other Hevea species. Locus duplication was observed in H. brasil-iensis and other species. Additionally, 43 SNP markers in 13 sequences that showed similarity to proteins involved in stress response, latex biosynthesis and developmental processes were characterized. cDNA libraries are a rich source of SSR and SNP markers and enable the identification of new transcripts. The new markers developed here will be a valuable resource for linkage mapping, QTL identification and other studies in the rubber tree and can also be used to evaluate the genetic variability of other Hevea species, which are valuable assets in rubber tree breeding. MenosHevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consuming and expensive, but molecular markers can serve as a tool for early valuation, thus reducing time and costs. In this work, we constructed six different cDNA libraries with the aim of developing gene-targeted molecular markers for the rubber tree. A total of 8,263 reads were assembled, generating 5,025 unigenes that were analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, and two sequences were highly up-regulated by cold stress. These unigenes were scanned for microsatellite (SSR) regions and single nucleotide polymorphisms (SNPs). In total, 169 novel EST-SSR markers were developed; 138 loci were polymorphic in the rubber tree, and 98 % presented transferability to six other Hevea species. Locus duplication was observed in H. brasil-iensis and other species. Additionally, 43 SNP markers in 13 sequences that showed similarity to proteins involved in stress response, latex biosynthesis and developmental processes were characterized. cDNA libraries are a rich source of SSR and SNP markers and enable the identification of new transcripts. The new markers developed here will be a valuable resource for linkage mapping, QTL identification and other studies in the r... Mostrar Tudo |
Palavras-Chave: |
Biblioteca cDNA; CDNA library; Fitomejoramiento; Marcador microssatélite; Marcador SNP; Marcadores genéticos; Repeticiones de microsatélite; Rubber tree. |
Thesagro: |
Caucho; DNA; Hevea brasiliensis; Marcador genético; Melhoramento genético vegetal; Seringueira. |
Thesaurus Nal: |
Genetic markers; Microsatellite repeats; Plant breeding. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/160345/1/25073.pdf
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Marc: |
LEADER 02997naa a2200409 a 4500 001 1988256 005 2021-07-06 008 2014 bl uuuu u00u1 u #d 022 $a1380-3743 (impresso) / 1572-9788 (on-line) 024 7 $a10.1007/s11032-014-0095-2$2DOI 100 1 $aSILVA, C. C. 245 $aLeaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions$bthe development of gene-targeted Functional markers for stress response.$h[electronic resource] 260 $c2014 520 $aHevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consuming and expensive, but molecular markers can serve as a tool for early valuation, thus reducing time and costs. In this work, we constructed six different cDNA libraries with the aim of developing gene-targeted molecular markers for the rubber tree. A total of 8,263 reads were assembled, generating 5,025 unigenes that were analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, and two sequences were highly up-regulated by cold stress. These unigenes were scanned for microsatellite (SSR) regions and single nucleotide polymorphisms (SNPs). In total, 169 novel EST-SSR markers were developed; 138 loci were polymorphic in the rubber tree, and 98 % presented transferability to six other Hevea species. Locus duplication was observed in H. brasil-iensis and other species. Additionally, 43 SNP markers in 13 sequences that showed similarity to proteins involved in stress response, latex biosynthesis and developmental processes were characterized. cDNA libraries are a rich source of SSR and SNP markers and enable the identification of new transcripts. The new markers developed here will be a valuable resource for linkage mapping, QTL identification and other studies in the rubber tree and can also be used to evaluate the genetic variability of other Hevea species, which are valuable assets in rubber tree breeding. 650 $aGenetic markers 650 $aMicrosatellite repeats 650 $aPlant breeding 650 $aCaucho 650 $aDNA 650 $aHevea brasiliensis 650 $aMarcador genético 650 $aMelhoramento genético vegetal 650 $aSeringueira 653 $aBiblioteca cDNA 653 $aCDNA library 653 $aFitomejoramiento 653 $aMarcador microssatélite 653 $aMarcador SNP 653 $aMarcadores genéticos 653 $aRepeticiones de microsatélite 653 $aRubber tree 700 1 $aMANTELLO, C. C. 700 1 $aCAMPOS, T. de 700 1 $aSOUZA, L. M. 700 1 $aGONÇALVES, P. S. 700 1 $aSOUZA, A. P. 773 $tMolecular Breeding, Berlim$gv. 34, n. 3, p. 1035-1053, Oct. 2014.
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Registro original: |
Embrapa Acre (CPAF-AC) |
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Registros recuperados : 6 | |
1. | | SILVA, C. C.; MANTELLO, C. C.; CAMPOS, T. de; SOUZA, L. M.; GONÇALVES, P. S.; SOUZA, A. P. Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted Functional markers for stress response. Molecular Breeding, Berlim, v. 34, n. 3, p. 1035-1053, Oct. 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Acre. |
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4. | | DERAMI, M. S.; MANTELLO, C. C.; SOUZA, A. P.; SOUSA, V. A. de; KESTRING, D. R.; AGUIAR, A. V. de; PENTEADO, S. do R. C. Development of microsatellite-enriched genomic library for Deladenus siricidicola. In: CONGRESSO BRASILEIRO DE GENÉTICA, 58., 2012, Foz do Iguaçu. Resumos... Foz do Iguaçú: SBG, 2012.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Florestas. |
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5. | | SOUZA, L. M. de; SANTOS, L. H. B. dos; ROSA, J. R. B. F.; SILVA, C. C. da; MANTELLO, C. C.; CONSON, A. R. O.; SCALOPPI JUNIOR, E. J.; FIALHO, J. de F.; MORAES, M. L. T. de; GONÇALVES, P. de S.; MARGARIDO, G. R. A.; GARCIA, A. A. F.; LE GUEN, V.; SOUZA, A. P. de. Linkage disequilibrium and population structure in wild and cultivated populations of rubber tree (Hevea brasiliensis). Frontiers in Plant Science, v. 9, article 815, 03 July 2018.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Cerrados. |
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6. | | SOUZA, L. M. de; LE GUEN, V.; CERQUEIRA-SILVA, C. B. M.; SILVA, C. C.; MANTELLO, C. C.; CONSON, A. R. O.; VIANNA, J. P. G.; ZUCCHI, M. I.; SCALOPPI JUNIOR, E. J.; FIALHO, J. de F.; MORAES, M. L. T. de; GONÇALVES, P. de S.; SOUZA, A. P. de. Genetic diversity strategy for the management and use of rubber genetic resources: more than 1,000 wild and cultivated accessions in a 100-Genotype core collection. Plos One, San Francisco, v. 10, n. 7, e013607, 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Cerrados. |
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Registros recuperados : 6 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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