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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
08/04/1997 |
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Data da última atualização: |
08/04/1997 |
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Autoria: |
MANFROI, V.; MARODIN, G. A. B.; SEIBERT, E.; ILHA, L. L. H.; MOLINOS, P. R. |
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Afiliação: |
Escola Agrotecnica Federal Pres. Juscelino Kubitschek, Bento Goncalves, RS. UFRGS, Porto Alegre. |
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Título: |
Quebra de dormencia e antecipacao da colheita em videira cv. Niagara Rosada. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Revista Brasileira de Fruticultura v. 18, n. 1, p. 65-74, 1996. |
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Idioma: |
Português |
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Conteúdo: |
O trabalho foi realizado na regiao metropolitana de Porto Alegre, RS, com o objetivo de avaliar a eficiencia da cianamida hidrogenada na quebra de dormencia e antecipacao da colheita em videira cv. Niagara Rosada. O delineamento experimental foi o de blocos casualizados, em parcelas subdivididas, com quatro repeticoes e tres plantas por subparcela. Nas parcelas foram alocadas as epocas de aplicacao e nas subparcelas as concentracoes de cianamida hidrogenada; as epocas de aplicao foram;epoca 1 - 11.07,1992, epoca 2 - 25.07.1992; as concentracoes de cianamida hidrogenada foram: 0% (testemunha), 0,49%, 0,98%, 1,47%. 1,96%, 2,45%. Concluiu-se que os tratamentos com ciabamida hidrogenada proporcionaram, em praticamente todos os casos, uma maior porcentagem de bro tacao das gemas, e a utilizacao de cianamida hidrogenada a partir da concentracao de 0,98% foi suficiente para estimular uma brotacao adequada.A producao de frutos de primeira qualidade foi aumentada pelo uso de cianamida, e as concentracoes maiores deste produto permitiram uma antecipacao consideravel na colheita das uvas de Niagara Rosada. |
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Palavras-Chave: |
Cianamida hidrogenada; Grape. |
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Thesagro: |
Maturação; Uva. |
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Thesaurus Nal: |
hydrogen cyanamide; ripening; Vitis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01788naa a2200253 a 4500
001 1634498
005 1997-04-08
008 1996 bl uuuu u00u1 u #d
100 1 $aMANFROI, V.
245 $aQuebra de dormencia e antecipacao da colheita em videira cv. Niagara Rosada.
260 $c1996
520 $aO trabalho foi realizado na regiao metropolitana de Porto Alegre, RS, com o objetivo de avaliar a eficiencia da cianamida hidrogenada na quebra de dormencia e antecipacao da colheita em videira cv. Niagara Rosada. O delineamento experimental foi o de blocos casualizados, em parcelas subdivididas, com quatro repeticoes e tres plantas por subparcela. Nas parcelas foram alocadas as epocas de aplicacao e nas subparcelas as concentracoes de cianamida hidrogenada; as epocas de aplicao foram;epoca 1 - 11.07,1992, epoca 2 - 25.07.1992; as concentracoes de cianamida hidrogenada foram: 0% (testemunha), 0,49%, 0,98%, 1,47%. 1,96%, 2,45%. Concluiu-se que os tratamentos com ciabamida hidrogenada proporcionaram, em praticamente todos os casos, uma maior porcentagem de bro tacao das gemas, e a utilizacao de cianamida hidrogenada a partir da concentracao de 0,98% foi suficiente para estimular uma brotacao adequada.A producao de frutos de primeira qualidade foi aumentada pelo uso de cianamida, e as concentracoes maiores deste produto permitiram uma antecipacao consideravel na colheita das uvas de Niagara Rosada.
650 $ahydrogen cyanamide
650 $aripening
650 $aVitis
650 $aMaturação
650 $aUva
653 $aCianamida hidrogenada
653 $aGrape
700 1 $aMARODIN, G. A. B.
700 1 $aSEIBERT, E.
700 1 $aILHA, L. L. H.
700 1 $aMOLINOS, P. R.
773 $tRevista Brasileira de Fruticultura$gv. 18, n. 1, p. 65-74, 1996.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
19/02/2016 |
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Data da última atualização: |
19/02/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, L. J.; DUARTE, A. W. F.; ROSA, L. H.; OLIVEIRA, V. M.; MELO, I. S. de. |
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Afiliação: |
L. J. SILVA, ESALQ/USP; A. W. F. DUARTE, USP; L. H. ROSA, UFMG; V. M. OLIVEIRA, CPQBA/Unicamp. |
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Título: |
Fosmid library: methodological challenges. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 0676-1. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Since 1983, Brazil conducts researchs in the Antartic continent through the Brazilian Antartic Program. For many years, studies were focused only to the understanding of the environmental impacts of climate change resulting from human action and the system of micro.- and macrorganisms. The interest of the bioprospecting molecules for use in agriculture or health began only in the last decade in the same continent. Since about half of the known bioactive compounds are originating from bacteria and fungi, and a tiny portion of the microbiota is cultivable in the Laboratory. Recent advances mainly to independent cultivation techniques allow a greater understanding of microbial biodiversity as well as access to functionality of their metabolic pathways. In this context the heterologous expression from metagenomic clones from total environmental DNA (eDNA) it stands out as a promising tool to access the microbial functional part. The objective of this study was to evaluate the efficiency of the cloning procedure (metagenomic library of clones in fosmid vector - pCC2FOS) according to different methodologies of obtaining eDNA, selection of the gene inserts and conditions for the reaction for ligation to vector. The samples were collected at Admiralty Bay - Antarctic Peninsula, during last summer (Nov/Feb 2014-2015.) by the MycoAntar Project. The samples were submitted to extraction of eDNA high molecular weight such as to mechanical and chemical cell lysis procedures. A portion of the product extraction was partitioned by pulsed field gel electrophoresis from which they were recovered fragments containing approximately 40Kb and the other portion went directly to the subsequent procedure. The ligation reaction was made in two different temperatures 16-25°C. The result of the chemical cell lysis reaction was more efficient at obtaining size fragments of interest for the cloning procedure despite the quantitative reduction compared to extraction by mechanical lysis. The recovery procedure of the inserts gel using gelase enzyme resulted in drastic losses at the final concentration of eDNA. In relation to the difference in incubation temperature the binding reaction some differences can be observed. Thus 15,000 clones were obtained and are currently preserved at -80°C for next experiments as well as be submitted to the enzymatic screening functional biological activities of biotechnological interest. MenosAbstract: Since 1983, Brazil conducts researchs in the Antartic continent through the Brazilian Antartic Program. For many years, studies were focused only to the understanding of the environmental impacts of climate change resulting from human action and the system of micro.- and macrorganisms. The interest of the bioprospecting molecules for use in agriculture or health began only in the last decade in the same continent. Since about half of the known bioactive compounds are originating from bacteria and fungi, and a tiny portion of the microbiota is cultivable in the Laboratory. Recent advances mainly to independent cultivation techniques allow a greater understanding of microbial biodiversity as well as access to functionality of their metabolic pathways. In this context the heterologous expression from metagenomic clones from total environmental DNA (eDNA) it stands out as a promising tool to access the microbial functional part. The objective of this study was to evaluate the efficiency of the cloning procedure (metagenomic library of clones in fosmid vector - pCC2FOS) according to different methodologies of obtaining eDNA, selection of the gene inserts and conditions for the reaction for ligation to vector. The samples were collected at Admiralty Bay - Antarctic Peninsula, during last summer (Nov/Feb 2014-2015.) by the MycoAntar Project. The samples were submitted to extraction of eDNA high molecular weight such as to mechanical and chemical cell lysis procedures. A p... Mostrar Tudo |
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Palavras-Chave: |
Antarctic peninsula; Metagenomic library; Unculturable microrganisms. |
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Categoria do assunto: |
V Taxonomia de Organismos |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139515/1/2015RA-008.pdf
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Marc: |
LEADER 03101nam a2200193 a 4500
001 2037800
005 2016-02-19
008 2015 bl uuuu u00u1 u #d
100 1 $aSILVA, L. J.
245 $aFosmid library$bmethodological challenges.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 0676-1.$c0676
520 $aAbstract: Since 1983, Brazil conducts researchs in the Antartic continent through the Brazilian Antartic Program. For many years, studies were focused only to the understanding of the environmental impacts of climate change resulting from human action and the system of micro.- and macrorganisms. The interest of the bioprospecting molecules for use in agriculture or health began only in the last decade in the same continent. Since about half of the known bioactive compounds are originating from bacteria and fungi, and a tiny portion of the microbiota is cultivable in the Laboratory. Recent advances mainly to independent cultivation techniques allow a greater understanding of microbial biodiversity as well as access to functionality of their metabolic pathways. In this context the heterologous expression from metagenomic clones from total environmental DNA (eDNA) it stands out as a promising tool to access the microbial functional part. The objective of this study was to evaluate the efficiency of the cloning procedure (metagenomic library of clones in fosmid vector - pCC2FOS) according to different methodologies of obtaining eDNA, selection of the gene inserts and conditions for the reaction for ligation to vector. The samples were collected at Admiralty Bay - Antarctic Peninsula, during last summer (Nov/Feb 2014-2015.) by the MycoAntar Project. The samples were submitted to extraction of eDNA high molecular weight such as to mechanical and chemical cell lysis procedures. A portion of the product extraction was partitioned by pulsed field gel electrophoresis from which they were recovered fragments containing approximately 40Kb and the other portion went directly to the subsequent procedure. The ligation reaction was made in two different temperatures 16-25°C. The result of the chemical cell lysis reaction was more efficient at obtaining size fragments of interest for the cloning procedure despite the quantitative reduction compared to extraction by mechanical lysis. The recovery procedure of the inserts gel using gelase enzyme resulted in drastic losses at the final concentration of eDNA. In relation to the difference in incubation temperature the binding reaction some differences can be observed. Thus 15,000 clones were obtained and are currently preserved at -80°C for next experiments as well as be submitted to the enzymatic screening functional biological activities of biotechnological interest.
653 $aAntarctic peninsula
653 $aMetagenomic library
653 $aUnculturable microrganisms
700 1 $aDUARTE, A. W. F.
700 1 $aROSA, L. H.
700 1 $aOLIVEIRA, V. M.
700 1 $aMELO, I. S. de
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Embrapa Meio Ambiente (CNPMA) |
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