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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
13/04/2011 |
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Data da última atualização: |
13/04/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
RAMIRO, D. A.; TOMA-BRAGHINI, M.; PETITOT, A. -S.; MALUF, M. P.; FERNANDEZ, D. |
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Afiliação: |
IRD/CIRAD/INRA; IRD/CIRAD/INRA; MIRIAN PEREZ MALUF, SAPC; IRD/CIRAD/INRA. |
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Título: |
Use of leaf-disk technique for gene expression analysis of the coffee responses to Hemileia vastatrix Infection. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM. |
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Idioma: |
Inglês |
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Conteúdo: |
The most acknowledged method for coffee leaf-rust resistance evaluation uses leaf disks inoculated with Hemileia vastatrix and kept in moisture chambers. Besides an efficient control of inoculation conditions, this technique allows a simultaneous evaluation of innumerous plants, with diverse fungal race/coffee genotype combinations, and using low uredospore quantities. The objective of this study was to evaluate the suitability of this technique for the functional gene analysis of coffee responses to leaf-rust infection. A comparison of gene expression in the presence or absence of the pathogen was performed on intact leaves and on leaf disks. To avoid non-specific gene expression due to leaf injury, the leaf disks were prepared 24h and 48h before inoculation and kept moist. Coffea arabica plant samples of the resistant Obatã and the susceptible Ouro Verde cultivars were challenged with H. vastatrix race II and were collected 24 h after inoculation. Semi-quantitative reverse transcription (RT)-PCR and real time quantitative PCR were used to evaluate expression of several coffee genes. Genes known to be constitutively expressed such as the Glyceraldehyde 3-phosphate deshydrogenase gene or the Ubiquitine gene were used, as well as genes involved in disease resistance responses. Results demonstrated that overall there are differences in the gene expression patterns observed in leaves and disks, either prepared 24h or 48h before inoculation. The genes PAD3 and PR1b showed induction in leaves and in the 48h-disks of Obatã upon rust fungus inoculation, and gene suppression in the 24h-disks treatment. The genes WRKYs were activated in leaves and suppressed in disks in the same cultivar. Opposite patterns of WRKY expression were detected in disks of Ouro Verde. Our results showed that most of the defense-related genes studied displayed altered patterns of gene expression compared to intact leaves. These results suggest that the leaf-disk technique cannot be successfully used for transcriptomic analysis of coffee-rust interactions. MenosThe most acknowledged method for coffee leaf-rust resistance evaluation uses leaf disks inoculated with Hemileia vastatrix and kept in moisture chambers. Besides an efficient control of inoculation conditions, this technique allows a simultaneous evaluation of innumerous plants, with diverse fungal race/coffee genotype combinations, and using low uredospore quantities. The objective of this study was to evaluate the suitability of this technique for the functional gene analysis of coffee responses to leaf-rust infection. A comparison of gene expression in the presence or absence of the pathogen was performed on intact leaves and on leaf disks. To avoid non-specific gene expression due to leaf injury, the leaf disks were prepared 24h and 48h before inoculation and kept moist. Coffea arabica plant samples of the resistant Obatã and the susceptible Ouro Verde cultivars were challenged with H. vastatrix race II and were collected 24 h after inoculation. Semi-quantitative reverse transcription (RT)-PCR and real time quantitative PCR were used to evaluate expression of several coffee genes. Genes known to be constitutively expressed such as the Glyceraldehyde 3-phosphate deshydrogenase gene or the Ubiquitine gene were used, as well as genes involved in disease resistance responses. Results demonstrated that overall there are differences in the gene expression patterns observed in leaves and disks, either prepared 24h or 48h before inoculation. The genes PAD3 and PR1b showed induct... Mostrar Tudo |
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Palavras-Chave: |
Coffee. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32618/1/Use-of-Leaf-Disk.pdf
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Marc: |
LEADER 02739nam a2200169 a 4500
001 1885780
005 2011-04-13
008 2007 bl uuuu u00u1 u #d
100 1 $aRAMIRO, D. A.
245 $aUse of leaf-disk technique for gene expression analysis of the coffee responses to Hemileia vastatrix Infection.$h[electronic resource]
260 $aIn:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM.$c2007
520 $aThe most acknowledged method for coffee leaf-rust resistance evaluation uses leaf disks inoculated with Hemileia vastatrix and kept in moisture chambers. Besides an efficient control of inoculation conditions, this technique allows a simultaneous evaluation of innumerous plants, with diverse fungal race/coffee genotype combinations, and using low uredospore quantities. The objective of this study was to evaluate the suitability of this technique for the functional gene analysis of coffee responses to leaf-rust infection. A comparison of gene expression in the presence or absence of the pathogen was performed on intact leaves and on leaf disks. To avoid non-specific gene expression due to leaf injury, the leaf disks were prepared 24h and 48h before inoculation and kept moist. Coffea arabica plant samples of the resistant Obatã and the susceptible Ouro Verde cultivars were challenged with H. vastatrix race II and were collected 24 h after inoculation. Semi-quantitative reverse transcription (RT)-PCR and real time quantitative PCR were used to evaluate expression of several coffee genes. Genes known to be constitutively expressed such as the Glyceraldehyde 3-phosphate deshydrogenase gene or the Ubiquitine gene were used, as well as genes involved in disease resistance responses. Results demonstrated that overall there are differences in the gene expression patterns observed in leaves and disks, either prepared 24h or 48h before inoculation. The genes PAD3 and PR1b showed induction in leaves and in the 48h-disks of Obatã upon rust fungus inoculation, and gene suppression in the 24h-disks treatment. The genes WRKYs were activated in leaves and suppressed in disks in the same cultivar. Opposite patterns of WRKY expression were detected in disks of Ouro Verde. Our results showed that most of the defense-related genes studied displayed altered patterns of gene expression compared to intact leaves. These results suggest that the leaf-disk technique cannot be successfully used for transcriptomic analysis of coffee-rust interactions.
653 $aCoffee
700 1 $aTOMA-BRAGHINI, M.
700 1 $aPETITOT, A. -S.
700 1 $aMALUF, M. P.
700 1 $aFERNANDEZ, D.
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Registro original: |
Embrapa Café (CNPCa) |
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| Registros recuperados : 69 | |
| 9. |  | BRANDALISE, M.; MALUF, M. P.; GUERREIRO FILHO, O.; GONÇALVES, W.; MAIA, I. de G. Caracterização de genes com expressão tecido-específica em raiz e folha de Coffea arabica. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 4., 2005, Londrina. Anais... Brasília, DF: Embrapa Café, 2005.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 10. | | OLIVEIRA, A. S. de; GOMES, L. H. M.; GOLDMAN, M. H. S.; MALUF, M. P. Caracterização de genes MADS em acessos de Coffea. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia, SP. Anais... Brasília, DF: Embrapa Café, 2007.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 11. | | SILVESTRINI, M.; MALUF, M. P.; RUGGIERO, L. M. de C.; GUERREIRO-FILHO, O.; COLOMBO, C. A. Caracterização de linhagens comerciais de café através de marcadores moleculares. IN: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 1., 2000, Poços de Caldas. Resumos Expandidos. Brasília, DF: Embrapa Café/MINASPLAN, 2000.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 16. | | MALUF, M. P.; AGUIAR, A. T. E.; GALLO, P. B.; FAZUOLI, L.; GUERREIRO FILHO, O. Caracterização agronômica e tecnológica de linhagens comerciais de café selecionadas pelo IAC. IN: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 1., 2000, Poços de Caldas. Resumos Expandidos. Brasília, DF: Embrapa Café/MINASPLAN, 2000.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 20. | | GALLEP, C. M.; AMARAL, E. M. do; ALONCO, G. C.; MALUF, M. P.; PADILHA, L. Germination vigour versus delayed luminescence of coffee seeds: preliminary series. In: INTERNATIONAL CONFERENCE ON PHOTONICS, OPTICS AND LASER TECHNOLOGY, 2., 2014, Lisboa. Photoptics 2014. Lisboa, 2014.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 69 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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