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 | Acesso ao texto completo restrito à biblioteca da Embrapa Café. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
17/03/2011 |
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Data da última atualização: |
28/10/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
SILVESTRINI, M.; RAMIRO, D. A.; GUERREIRO-FILHO, O.; MALUF, M. P. |
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Afiliação: |
Coffee Center "Alcides Carvalho"; IAC; IAC; MIRIAN PEREZ MALUF, SAPC. |
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Título: |
Defense-related gene expression in response to leaf-miner infection. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas. |
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Idioma: |
Inglês |
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Conteúdo: |
In Brazilian coffee plantations, the leaf-miner (Leucoptera coffeella) represents a major threat. Insect control is mainly based on the use of pesticides, as resistant cultivars are not yet available. However, the coffee breeding program of IAC has already selected promising genotypes bearing resistance to the leaf-miner, which was transferred from the diploid species Coffea racemosa. In order to characterize molecular aspects of defense mechanisms associated to the resistance response, expression of key genes was evaluated in coffee leaves, from both resistant and susceptible plants, in response to leaf-miner infection. Infected leavesb were collected at different time-intervals during insect development. Defense-related genes were identified in the Coffee Genome Database, through Blast searches. Gene specific primers were used to amplify corresponding transcripts on sampled leaves using a quantitative RT-PCR approach. Results indicated that there are no significant differences in the expression patterns of evaluated genes when comparing resistant and susceptible infected leaves. Major differences were observed for lipoxygenase, glutathione transferase, protein-kinase receptor and glucanase. However, these differences are mainly associated with expression timing along insect infection rather than with gene regulation, suggesting that resistance to leafminer in coffee may be associated with a previously built basal defense-response. |
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Thesagro: |
Leucoptera Coffeella. |
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Categoria do assunto: |
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Marc: |
LEADER 01958nam a2200157 a 4500
001 1881314
005 2011-10-28
008 2008 bl uuuu u00u1 u #d
100 1 $aSILVESTRINI, M.
245 $aDefense-related gene expression in response to leaf-miner infection.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas.$c2008
520 $aIn Brazilian coffee plantations, the leaf-miner (Leucoptera coffeella) represents a major threat. Insect control is mainly based on the use of pesticides, as resistant cultivars are not yet available. However, the coffee breeding program of IAC has already selected promising genotypes bearing resistance to the leaf-miner, which was transferred from the diploid species Coffea racemosa. In order to characterize molecular aspects of defense mechanisms associated to the resistance response, expression of key genes was evaluated in coffee leaves, from both resistant and susceptible plants, in response to leaf-miner infection. Infected leavesb were collected at different time-intervals during insect development. Defense-related genes were identified in the Coffee Genome Database, through Blast searches. Gene specific primers were used to amplify corresponding transcripts on sampled leaves using a quantitative RT-PCR approach. Results indicated that there are no significant differences in the expression patterns of evaluated genes when comparing resistant and susceptible infected leaves. Major differences were observed for lipoxygenase, glutathione transferase, protein-kinase receptor and glucanase. However, these differences are mainly associated with expression timing along insect infection rather than with gene regulation, suggesting that resistance to leafminer in coffee may be associated with a previously built basal defense-response.
650 $aLeucoptera Coffeella
700 1 $aRAMIRO, D. A.
700 1 $aGUERREIRO-FILHO, O.
700 1 $aMALUF, M. P.
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Registro original: |
Embrapa Café (CNPCa) |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Milho e Sorgo; Embrapa Unidades Centrais. |
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Data corrente: |
30/07/2013 |
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Data da última atualização: |
16/09/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CARVALHO, M. T. de M.; MADARI, B. E.; LEAL, W. G. de O.; COSTA, A. R. da; MACHADO, P. L. O. de A.; SILVEIRA, P. M. da; MOREIRA, J. A. A.; HEINEMANN, A. B. |
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Afiliação: |
MARCIA THAIS DE MELO CARVALHO, CNPAF; BEATA EMOKE MADARI, CNPAF; WESLEY GABRIEL DE OLIVEIRA LEAL, CNPAF; ADRIANA RODOLFO DA COSTA, UEG; PEDRO LUIZ OLIVEIRA DE A MACHADO, CNPAF; PEDRO MARQUES DA SILVEIRA, CNPAF; JOSE ALOISIO ALVES MOREIRA, CNPMS; ALEXANDRE BRYAN HEINEMANN, CNPAF. |
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Título: |
Nitrogen fluxes from irrigated common-bean as affected by mulching and mineral fertilization. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, v. 48, n. 5, p. 478-486, maio 2013. |
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ISSN: |
0100-204X |
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DOI: |
10.1590/S0100-204X2013000500003 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this work was to measure the fluxes of N2O‑N and NH3‑N throughout the growing season of irrigated common‑bean (Phaseolus vulgaris), as affected by mulching and mineral fertilization. Fluxes of N2O‑N and NH3‑N were evaluated in areas with or without Congo signal grass mulching (Urochloa ruziziensis) or mineral fertilization. Fluxes of N were also measured in a native Cerrado area, which served as reference. Total N2O‑N and NH3‑N emissions were positively related to the increasing concentrations of moisture, ammonium, and nitrate in the crop system, within 0.5 m soil depth. Carbon content in the substrate and microbial biomass within 0.1 m soil depth were favoured by Congo signal grass and related to higher emissions of N2O‑N, regardless of N fertilization. Emission factors (N losses from the applied mineral nitrogen) for N2O‑N (0.01?0.02%) and NH3‑N (0.3?0.6%) were lower than the default value recognized by the Intergovernmental Panel on Climate Change. Mulch of Congo signal grass benefits N2O‑N emission regardless of N fertilization. |
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Palavras-Chave: |
Gás de efeito estufa; N fertilization; No?tillage; Óxido nitroso. |
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Thesagro: |
Brachiaria; Cerrado; Cobertura morta; Efeito estufa; Feijão; Fertilidade do solo; Mudança Climática; Nitrogênio; Óxido; Phaseolus vulgaris; Plantio direto. |
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Thesaurus NAL: |
Climate change; Greenhouse gases; Nitrous oxide. |
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Categoria do assunto: |
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F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/88351/1/pab.pdf
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Marc: |
LEADER 02392naa a2200445 a 4500
001 1964556
005 2022-09-16
008 2013 bl uuuu u00u1 u #d
022 $a0100-204X
024 7 $a10.1590/S0100-204X2013000500003$2DOI
100 1 $aCARVALHO, M. T. de M.
245 $aNitrogen fluxes from irrigated common-bean as affected by mulching and mineral fertilization.
260 $c2013
520 $aThe objective of this work was to measure the fluxes of N2O‑N and NH3‑N throughout the growing season of irrigated common‑bean (Phaseolus vulgaris), as affected by mulching and mineral fertilization. Fluxes of N2O‑N and NH3‑N were evaluated in areas with or without Congo signal grass mulching (Urochloa ruziziensis) or mineral fertilization. Fluxes of N were also measured in a native Cerrado area, which served as reference. Total N2O‑N and NH3‑N emissions were positively related to the increasing concentrations of moisture, ammonium, and nitrate in the crop system, within 0.5 m soil depth. Carbon content in the substrate and microbial biomass within 0.1 m soil depth were favoured by Congo signal grass and related to higher emissions of N2O‑N, regardless of N fertilization. Emission factors (N losses from the applied mineral nitrogen) for N2O‑N (0.01?0.02%) and NH3‑N (0.3?0.6%) were lower than the default value recognized by the Intergovernmental Panel on Climate Change. Mulch of Congo signal grass benefits N2O‑N emission regardless of N fertilization.
650 $aClimate change
650 $aGreenhouse gases
650 $aNitrous oxide
650 $aBrachiaria
650 $aCerrado
650 $aCobertura morta
650 $aEfeito estufa
650 $aFeijão
650 $aFertilidade do solo
650 $aMudança Climática
650 $aNitrogênio
650 $aÓxido
650 $aPhaseolus vulgaris
650 $aPlantio direto
653 $aGás de efeito estufa
653 $aN fertilization
653 $aNo?tillage
653 $aÓxido nitroso
700 1 $aMADARI, B. E.
700 1 $aLEAL, W. G. de O.
700 1 $aCOSTA, A. R. da
700 1 $aMACHADO, P. L. O. de A.
700 1 $aSILVEIRA, P. M. da
700 1 $aMOREIRA, J. A. A.
700 1 $aHEINEMANN, A. B.
773 $tPesquisa Agropecuária Brasileira$gv. 48, n. 5, p. 478-486, maio 2013.
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Embrapa Arroz e Feijão (CNPAF) |
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