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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
05/01/2016 |
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Data da última atualização: |
17/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BOSCO, D. D.; SINSKI, I.; COMACHIO, V.; MAIA, J. D. G.; RITSCHEL, P. S.; QUECINI, V. |
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Afiliação: |
DANIELA DAL BOSCO, CNPUV; IRACI SINSKI, CNPUV; VALTAIR COMACHIO, CNPUV; JOAO DIMAS GARCIA MAIA, CNPUV; PATRICIA SILVA RITSCHEL, CNPUV; VERA MARIA QUECINI, CNPUV. |
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Título: |
In Vitro techniques for grapevine germplasm conservation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Acta Horticultura, n. 1082, Apr. 2015. p. 201-206. |
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Idioma: |
Português |
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Conteúdo: |
Abstract Plant genetic resources hold significant phenotypic variation resultant from the presence of allelic diversity, which is maintained by evolutionary processes or by artificial selection. Therefore, plant germplasm encompasses the huge genotypic diversity found in wild and cultivated species and constitutes a source of interesting traits for breeders. Although extremely valuable, biodiversity conservation has high demand for funding, physical space and labor. In vitro conservation is an interesting alternative for the maintenance of highly-heterozygous, vegetatively propagated, perennial species, such as grapevine. It also contributes to the plants? phytosanitary conditions. The current work aimed to develop effective and feasible means toward in vitro establishment and conservation of grapevine germplasm. Woody stakes were obtained from the field collection of the Grapevine Germplasm Bank, at Embrapa, and were surface disinfected, planted in a mixture of autoclaved soil and vermiculite (1:1), and kept under controlled temperature (23±5°C) and relative humidity (70%). Young apical shoots were excised and superficially disinfected in the presence of 1% (w/v) polyvinylpirrolidone. Explants were transferred to tubes containing Galzy medium with active charcoal, under aseptic conditions. Established plants were propagated and maintained in vitro as duplicates. For long-term conservation, the effectiveness of two cryopreservation techniques; vitrification and encapsulationdehydration, was compared for 11 grapevine genotypes, including Vitis vinifera, V. labrusca and hybrids V. berlandieri × V. rupestris, and V. riparia × V. berlandieri. Shoot induction from treated stakes under protected greenhouse conditions significantly reduced environmental contamination and, along with the use of antioxidant agents, allowed in vitro establishment of approximately 1200 (85% of the accessions held by the bank) grapevine accessions. The establishment of the remaining accessions is underway. Plants free of ectophytes were produced for 900 (64.3%) accessions. Cryogenic protocols require further adjustments to allow acceptable recovery rates. High-scale in vitro conservation of grapevine germplasm is feasible and may safeguard valuable biodiversity. Although promising, cryopreservation requires further studies for protocol optimization. MenosAbstract Plant genetic resources hold significant phenotypic variation resultant from the presence of allelic diversity, which is maintained by evolutionary processes or by artificial selection. Therefore, plant germplasm encompasses the huge genotypic diversity found in wild and cultivated species and constitutes a source of interesting traits for breeders. Although extremely valuable, biodiversity conservation has high demand for funding, physical space and labor. In vitro conservation is an interesting alternative for the maintenance of highly-heterozygous, vegetatively propagated, perennial species, such as grapevine. It also contributes to the plants? phytosanitary conditions. The current work aimed to develop effective and feasible means toward in vitro establishment and conservation of grapevine germplasm. Woody stakes were obtained from the field collection of the Grapevine Germplasm Bank, at Embrapa, and were surface disinfected, planted in a mixture of autoclaved soil and vermiculite (1:1), and kept under controlled temperature (23±5°C) and relative humidity (70%). Young apical shoots were excised and superficially disinfected in the presence of 1% (w/v) polyvinylpirrolidone. Explants were transferred to tubes containing Galzy medium with active charcoal, under aseptic conditions. Established plants were propagated and maintained in vitro as duplicates. For long-term conservation, the effectiveness of two cryopreservation techniques; vitrification and encapsulation... Mostrar Tudo |
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Palavras-Chave: |
In vitro; Phytosanitary improvement. |
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Thesaurus Nal: |
Germplasm; Micropropagation; Tissue culture. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136629/1/Ritschel-Actahort-n1082-Apr2015.pdf
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Marc: |
LEADER 03023naa a2200241 a 4500
001 2032995
005 2016-03-17
008 2015 bl uuuu u00u1 u #d
100 1 $aBOSCO, D. D.
245 $aIn Vitro techniques for grapevine germplasm conservation.$h[electronic resource]
260 $c2015
520 $aAbstract Plant genetic resources hold significant phenotypic variation resultant from the presence of allelic diversity, which is maintained by evolutionary processes or by artificial selection. Therefore, plant germplasm encompasses the huge genotypic diversity found in wild and cultivated species and constitutes a source of interesting traits for breeders. Although extremely valuable, biodiversity conservation has high demand for funding, physical space and labor. In vitro conservation is an interesting alternative for the maintenance of highly-heterozygous, vegetatively propagated, perennial species, such as grapevine. It also contributes to the plants? phytosanitary conditions. The current work aimed to develop effective and feasible means toward in vitro establishment and conservation of grapevine germplasm. Woody stakes were obtained from the field collection of the Grapevine Germplasm Bank, at Embrapa, and were surface disinfected, planted in a mixture of autoclaved soil and vermiculite (1:1), and kept under controlled temperature (23±5°C) and relative humidity (70%). Young apical shoots were excised and superficially disinfected in the presence of 1% (w/v) polyvinylpirrolidone. Explants were transferred to tubes containing Galzy medium with active charcoal, under aseptic conditions. Established plants were propagated and maintained in vitro as duplicates. For long-term conservation, the effectiveness of two cryopreservation techniques; vitrification and encapsulationdehydration, was compared for 11 grapevine genotypes, including Vitis vinifera, V. labrusca and hybrids V. berlandieri × V. rupestris, and V. riparia × V. berlandieri. Shoot induction from treated stakes under protected greenhouse conditions significantly reduced environmental contamination and, along with the use of antioxidant agents, allowed in vitro establishment of approximately 1200 (85% of the accessions held by the bank) grapevine accessions. The establishment of the remaining accessions is underway. Plants free of ectophytes were produced for 900 (64.3%) accessions. Cryogenic protocols require further adjustments to allow acceptable recovery rates. High-scale in vitro conservation of grapevine germplasm is feasible and may safeguard valuable biodiversity. Although promising, cryopreservation requires further studies for protocol optimization.
650 $aGermplasm
650 $aMicropropagation
650 $aTissue culture
653 $aIn vitro
653 $aPhytosanitary improvement
700 1 $aSINSKI, I.
700 1 $aCOMACHIO, V.
700 1 $aMAIA, J. D. G.
700 1 $aRITSCHEL, P. S.
700 1 $aQUECINI, V.
773 $tActa Horticultura$gn. 1082, Apr. 2015. p. 201-206.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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| Registros recuperados : 215 | |
| 4. |  | MAIA, J. D. G. Banco ativo de germoplasma de uva: Embrapa Uva e Vinho. In: PADUA, J. G.; ALBUQUERQUE, M. do S. M.; MELLO, S. C. M. de (Ed.). Bancos e coleções de germoplasma da Embrapa: conservação e uso. Brasília: Embrapa Recursos Genéticos e Biotecnologia, 2020. p. 108. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 371).| Biblioteca(s): Embrapa Uva e Vinho. |
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| 5. | | MAIA, J. D. G. Avaliação fenológica e glucométrica de germoplasma de videira. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 4., 2016, Curitiba, PR. Anais...Recursos Genéticos no Brasil: a base para o desenvolvimento sustentável, Curitiba, RS: Instituto Agronômico do Paraná e Sociedade Brasileira de Recursos Genéticos, de 8 a 11 nov. 2016, p. 227.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 7. | | MAIA, J. D. G. Manejo da videira Niágara Rosada em regiões tropicais. In: SIMPÓSIO MINEIRO DE VITICULTURA E ENOLOGIA, 1., 2002, Andradas, MG. Viticultura e Enologia: atualizando conceitos. Caldas: EPAMIG, 2002. p. 49-58 il. Coordenado por Murilo Albuquerque Regina com colaboração de Luiz Eduardo Correa Antunes.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 11. | | MAIA, J. D. G. Origem da videira Niágara. In: MAIA, J. D. G.; CAMARGO, U. A. (Ed.). O cultivo da videira Niágara no Brasil. Brasília, DF: Embrapa, 2012. p. 13-22.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 18. | | MAIA, J. D. G.; RITSCHEL, P. S. Avaliação de germoplasma de videira em condições de clima tropical úmido. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 4., 2016, Curitiba, PR. Anais...Recursos Genéticos no Brasil: a base para o desenvolvimento sustentável, Curitiba, RS: Instituto Agronômico do Paraná e Sociedade Brasileira de Recursos Genéticos, de 8 a 11 nov. 2016, p. 217.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 20. | | MAIA, J. D. G.; CAMARGO, U. A. Implantação do vinhedo. In: MAIA, J. D. G.; CAMARGO, U. A. (Ed.). O cultivo da videira Niágara no Brasil. Brasília, DF: Embrapa, 2012. p. 31-79.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| Registros recuperados : 215 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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