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Registro Completo |
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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
18/03/2021 |
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Data da última atualização: |
18/03/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROSSATTO, T.; AULER, P. A.; AMARAL, M. N.; MILECH, C.; MAGALHAES JUNIOR, A. M. de; BRAGA, E. J. B. |
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Afiliação: |
T. ROSSATTO, UFPEL; P. A. AULER, UFPEL; M. N. AMARAL, UFPEL; C. MILECH, UFPEL; ARIANO MARTINS DE MAGALHAES JUNIOR, CPACT; E. J. B. BRAGA, UFPEL. |
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Título: |
Selection of Reference Genes for RT-qPCR Studies in Different Organs of Rice Cultivar BRS AG Submitted to Recurrent Saline Stress. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Russian Journal of Plant Physiology, v. 68, n. 2, p. 254-265, 2021. |
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ISSN: |
1021-4437 |
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Idioma: |
Inglês |
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Conteúdo: |
Quantitative real-time polymerase chain reactions (RT-qPCR) have become one of the most widely used methods for analyzing gene expression, provided suitable reference genes are available to normalize the data. RNA was isolated from leaves, grain, rachises and sheaths of rice (Oryza sativa L. cv. BRS AG) submitted to different saline stress events for seven days, and expression analysis was carried out by RT-qPCR. Expression levels of ten candidate reference genes were assessed, actin11 (ACT11), ubiquitin conjugating enzyme E2 (UBC-E2), eukaryotic elongation factor1-a (Eef-1a), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), B-tubulin (B-Tub), eukaryotic initiation factor 4a (Eif-4-a), ubiquitin10 (UBQ10), ubiquitin5 (UBQ5), aquaporin TIP41 (TIP41-like). Gene expression stability was calculated using the common statistical algorithms geNorm, BestKeeper and ?Ct method, NormFinder and RefFinder. The most stably expressed genes were UBC2E and GAPDH for leaves, UBQ5 and UBQ10 for sheaths, TIP41 and UBQ10 for rachises, and TIP41 and cyclophilin for grain. Gene expression of triose phosphate translocator (TPT1), ADP-glucose transporter (BT1-1), choline monooxygenase (CMO) was used to validate the selected reference genes. The results highlighted the importance of using suitable reference gene to normalize gene expression data in rice plants. |
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Thesagro: |
Arroz; Gene; Oryza Sativa. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/221965/1/Artigo-Selection-of-Reference-Genes.pdf
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Marc: |
LEADER 02060naa a2200229 a 4500
001 2130778
005 2021-03-18
008 2021 bl uuuu u00u1 u #d
022 $a1021-4437
100 1 $aROSSATTO, T.
245 $aSelection of Reference Genes for RT-qPCR Studies in Different Organs of Rice Cultivar BRS AG Submitted to Recurrent Saline Stress.$h[electronic resource]
260 $c2021
520 $aQuantitative real-time polymerase chain reactions (RT-qPCR) have become one of the most widely used methods for analyzing gene expression, provided suitable reference genes are available to normalize the data. RNA was isolated from leaves, grain, rachises and sheaths of rice (Oryza sativa L. cv. BRS AG) submitted to different saline stress events for seven days, and expression analysis was carried out by RT-qPCR. Expression levels of ten candidate reference genes were assessed, actin11 (ACT11), ubiquitin conjugating enzyme E2 (UBC-E2), eukaryotic elongation factor1-a (Eef-1a), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), B-tubulin (B-Tub), eukaryotic initiation factor 4a (Eif-4-a), ubiquitin10 (UBQ10), ubiquitin5 (UBQ5), aquaporin TIP41 (TIP41-like). Gene expression stability was calculated using the common statistical algorithms geNorm, BestKeeper and ?Ct method, NormFinder and RefFinder. The most stably expressed genes were UBC2E and GAPDH for leaves, UBQ5 and UBQ10 for sheaths, TIP41 and UBQ10 for rachises, and TIP41 and cyclophilin for grain. Gene expression of triose phosphate translocator (TPT1), ADP-glucose transporter (BT1-1), choline monooxygenase (CMO) was used to validate the selected reference genes. The results highlighted the importance of using suitable reference gene to normalize gene expression data in rice plants.
650 $aArroz
650 $aGene
650 $aOryza Sativa
700 1 $aAULER, P. A.
700 1 $aAMARAL, M. N.
700 1 $aMILECH, C.
700 1 $aMAGALHAES JUNIOR, A. M. de
700 1 $aBRAGA, E. J. B.
773 $tRussian Journal of Plant Physiology$gv. 68, n. 2, p. 254-265, 2021.
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Registro original: |
Embrapa Clima Temperado (CPACT) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Ocidental. Para informações adicionais entre em contato com cpaa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
19/12/2018 |
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Data da última atualização: |
02/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
RIBEIRO, D. G.; ALMEIDA, R. F. de; FONTES, W.; CASTRO, M. de S.; SOUSA, M. V. de; RICART, C. A. O.; CUNHA, R. N. V. da; LOPES, R.; PEREIRA, J. E. S.; REIS, A. M. dos. |
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Afiliação: |
Daiane Gonzaga Ribeiro; Raphael Ferreira de Almeida; Wagner Fontes, University of Brasília; Mariana de Souza Castro, University of Brasília; Marcelo Valle de Sousa, University of Brasília; Carlos André Ornelas Ricart, University of Brasília; RAIMUNDO NONATO VIEIRA DA CUNHA, CPAA; RICARDO LOPES, CPAA; JONNY EVERSON SCHERWINSKI PEREIRA, Cenargen; ANGELA MEHTA DOS REIS, Cenargen. |
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Título: |
Stress and cell cycle regulation during somatic embryogenesis plays a key role in oil palm callus development. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Proteomics, v. 192, p. 137-146, 2019. |
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DOI: |
https://doi-org.ez103.periodicos.capes.gov.br/10.1016/j.jprot.2018.08.015 |
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Idioma: |
Inglês |
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Conteúdo: |
il palm is an oleaginous plant of relevant economic importance since its fruits are rich in vegetable oil. These plants have a single apical meristem and the main method for vegetative propagation is somatic embryogenesis. The aim of this study was to identify differentially abundant proteins from oil palm genotypes contrasting in the capacity of embryogenic competence acquisition, using shotgun proteomics. Oil palm leaves were subjected to callus induction and the material was collected in biological triplicates at 14 and 90 days of callus induction. |
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Palavras-Chave: |
Embryogenic competence; Palma de óleo. |
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Thesagro: |
Elaeis Guineensis; Elaeis Oleifera. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01480naa a2200289 a 4500
001 2102086
005 2019-12-02
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi-org.ez103.periodicos.capes.gov.br/10.1016/j.jprot.2018.08.015$2DOI
100 1 $aRIBEIRO, D. G.
245 $aStress and cell cycle regulation during somatic embryogenesis plays a key role in oil palm callus development.$h[electronic resource]
260 $c2019
520 $ail palm is an oleaginous plant of relevant economic importance since its fruits are rich in vegetable oil. These plants have a single apical meristem and the main method for vegetative propagation is somatic embryogenesis. The aim of this study was to identify differentially abundant proteins from oil palm genotypes contrasting in the capacity of embryogenic competence acquisition, using shotgun proteomics. Oil palm leaves were subjected to callus induction and the material was collected in biological triplicates at 14 and 90 days of callus induction.
650 $aElaeis Guineensis
650 $aElaeis Oleifera
653 $aEmbryogenic competence
653 $aPalma de óleo
700 1 $aALMEIDA, R. F. de
700 1 $aFONTES, W.
700 1 $aCASTRO, M. de S.
700 1 $aSOUSA, M. V. de
700 1 $aRICART, C. A. O.
700 1 $aCUNHA, R. N. V. da
700 1 $aLOPES, R.
700 1 $aPEREIRA, J. E. S.
700 1 $aREIS, A. M. dos
773 $tJournal of Proteomics$gv. 192, p. 137-146, 2019.
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Embrapa Amazônia Ocidental (CPAA) |
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