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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
16/12/1992 |
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Data da última atualização: |
10/08/2004 |
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Autoria: |
BEACHY, R. N.; THOMPSON, J. F.; MADISON, J. T. |
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Título: |
Isolation and characterization of messenger RNAs that code for the subunits of soybean seed protein. |
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Ano de publicação: |
1979 |
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Fonte/Imprenta: |
In: RUBENSTEIN, I.; PHILLIPS, R.L.; GREEN, C.E.; GENGENBACH, B.G. The plant seed: development, preservation, and germination. New York : Academic Press, 1979. |
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Páginas: |
p.67-84. |
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Idioma: |
Inglês |
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Conteúdo: |
The storage proteins of soybean characteristically sediment in sucrose density gradients with coefficients of aproximately 7S (conglycinin) and 11S (glycinin). With sodium dodecyl sulfate and Beta-mercaptoethanol treatment the 7S protein breaks down into at least three polypeptide subunits, while the 11S protein breaks into four basic and four acidic subunits. Messenger RNAs have been isolated from polyribosomes taken from developing soybean seeds and after affinity chromatography on oligo dT-cellulose and sucrose density gradient centrifugation (under denaturing conditions) where translated in a wheat germ cellfree protein synthesizing system. Messenger RNA activities ranged from approximately 10S to 24S. Some, but not all, of the polypeptides synthesized invitro had electrophoretic mobilities identical with some of the subunits of the storage proteins. Preliminary evidence from tryptic fragment analysis suggested that the basic subunits of the 11S protein are synthesized in vitro; other products have not been comparably characterized. Polyacrylamide gel electrophoretic analysis of the poly(A) + RNAs showed a widely heterodisperse population of mRNAs with no predominating species. |
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Palavras-Chave: |
Protein; Seed; Soybean. |
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Thesagro: |
Proteína; Semente; Soja. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01910naa a2200229 a 4500
001 1451432
005 2004-08-10
008 1979 bl uuuu u00u1 u #d
100 1 $aBEACHY, R. N.
245 $aIsolation and characterization of messenger RNAs that code for the subunits of soybean seed protein.
260 $c1979
300 $ap.67-84.
520 $aThe storage proteins of soybean characteristically sediment in sucrose density gradients with coefficients of aproximately 7S (conglycinin) and 11S (glycinin). With sodium dodecyl sulfate and Beta-mercaptoethanol treatment the 7S protein breaks down into at least three polypeptide subunits, while the 11S protein breaks into four basic and four acidic subunits. Messenger RNAs have been isolated from polyribosomes taken from developing soybean seeds and after affinity chromatography on oligo dT-cellulose and sucrose density gradient centrifugation (under denaturing conditions) where translated in a wheat germ cellfree protein synthesizing system. Messenger RNA activities ranged from approximately 10S to 24S. Some, but not all, of the polypeptides synthesized invitro had electrophoretic mobilities identical with some of the subunits of the storage proteins. Preliminary evidence from tryptic fragment analysis suggested that the basic subunits of the 11S protein are synthesized in vitro; other products have not been comparably characterized. Polyacrylamide gel electrophoretic analysis of the poly(A) + RNAs showed a widely heterodisperse population of mRNAs with no predominating species.
650 $aProteína
650 $aSemente
650 $aSoja
653 $aProtein
653 $aSeed
653 $aSoybean
700 1 $aTHOMPSON, J. F.
700 1 $aMADISON, J. T.
773 $tIn: RUBENSTEIN, I.; PHILLIPS, R.L.; GREEN, C.E.; GENGENBACH, B.G. The plant seed: development, preservation, and germination. New York : Academic Press, 1979.
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Registro original: |
Embrapa Soja (CNPSO) |
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