Data/hora: 01/06/2024 07:37:48

Biblioteca(s): 	Embrapa Hortaliças.	
Data corrente: 	26/10/2023	
Data da última atualização: 	26/10/2023	
Autoria: 	MORAES, G. S. de; MELLO, J. F. de; BRITO, A. C. de Q.; REIS, A.; MICHEREFF, S. J.; GOMES, A. A. M.; CÂMARA, M. P. S.; VIEIRA, J. C. B; SOUZA-MOTA, C. M. de; MACHADO, A. R.	
Afiliação: 	GLÍCIA SILVA DE MORAES, UNIVERSIDADE FEDERAL DE PERNAMBUCO; JULIANA FERREIRA DE MELLO, UNIVERSIDADE FEDERAL DE PERNAMBUCO; AMANDA CUPERTINO DE QUEIROZ BRITO, UNIVERSIDADE FEDERAL DE PERNAMBUCO; AILTON REIS, CNPH; SAMI JORGE MICHEREFF, UNIVERSIDADE FEDERAL DE PERNAMBUCO; ANDRÉ ANGELO MEDEIROS GOMES, UNIVERSIDADE FEDERAL DE PERNAMBUCO; MARCOS PAZ SARAIVA CÂMARA, UNIVERSIDADE FEDERAL DE PERNAMBUCO; JANIELE CÁSSIA BARBOSA VIEIRA, UNIVERSIDADE FEDERAL DE PERNAMBUCO; CRISTINA MARIA DE SOUZA-MOTA, UNIVERSIDADE FEDERAL DE PERNAMBUCO; ALEXANDRE REIS MACHADO, UNIVERSIDADE FEDERAL DE PERNAMBUCO.	
Título: 	Phylogeny of Pseudoperonospora causing downy mildew of Cucurbitaceae in Brazil.	
Ano de publicação: 	2023	
Fonte/Imprenta: 	European Journal of Plant Pathology, v. 167, p. 361-370, 2023.	
DOI: 	https://doi.org/10.1007/s10658-023-02717-9	
Idioma: 	Inglês	
Conteúdo: 	Downy mildew of cucurbits is a disease caused by the Oomycete Pseudoperonospora cubensis. It can cause considerable losses in cucurbit crops. Phylogenetic studies point to the existence of cryptic species of Pseudoperonospora. The objective of this study was to determine, the phylogeny of Pseudoperonospora species afecting cucurbits in diferent regions of Brazil. For this, leaves with symptoms of downy mildew were collected in areas of cucurbits production in the states of Pernambuco, Minas Gerais, Ceará, and the Distrito Federal. For phylogenetic analyses, DNA extraction was performed, and PCR reactions were carried out using primers DC-6 and LR-0, Cox2-F and Cox2-RC4, OomCox1-levup and OomCox1-levlo, FM79-C1 and FMPHY-10b, for amplifcation of the internal transcribed spacer (ITS) region, the mitochondrial cytochrome c oxidase subunit II (cox2), the mitochondrial cytochrome c oxidase subunit I (cox1) and cox2-cox1 intergenic spacer respectively. The nucleotide sequences were analyzed, edited, and aligned to perform Bayesian Inference analysis. The results of the phylogenetic analyses presented in the cox2-cox1-cox2_1-ITS concatenated tree indicate that in Brazil, the downy mildew of cucurbits is caused by phylogenetically distinct lineages of P. cubensis.	
Palavras-Chave: 	Oomiceto Pseudoperonospora.	
Thesagro: 	Cucumis Melo; Cucumis Sativus.	
Thesaurus Nal: 	Genetic variation; Oomycetes; Peronosporaceae; Pseudoperonospora cubensis; Watermelons.	
Categoria do assunto: 	F Plantas e Produtos de Origem Vegetal	
Registro original: 	Embrapa Hortaliças (CNPH)	
Exemplares: 	CNPH - (41916-1) - UPC - AP - DD -  -  -  - DIS