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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
18/01/2024 |
Data da última atualização: |
18/01/2024 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
NOGUEIRA, C. P.; ADRIANO, L. H. C.; MOREIRA, O. B. de O.; OLIVEIRA, M. A. L. de; MACHADO, J. C. |
Afiliação: |
CECILIA PINTO NOGUEIRA, CNPGL; LUIZ HENRIQUE CANTARINO ADRIANO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; OLIVIA BRITO DE OLIVEIRA MOREIRA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; MARCONE AUGUSTO LEAL DE OLIVEIRA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; JUAREZ CAMPOLINA MACHADO, CNPGL. |
Título: |
Perfil de extração de antocianinas em genótipo de capim elefante roxo |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 12., 2023, Caxambu, MG. Anais. Piracicaba: Sociedade Brasileira de Melhoramento de Plantas, 2023. |
Idioma: |
Português |
Conteúdo: |
O capim-elefante roxo [Cenchrus purpureus (Schumach.) Morrone] tem a cor característica exibida pelos vegetais que possuem antocianinas que são um corante natural que apresenta efeito contra doenças cardiovasculares, neurodegenerativas e que pode prevenir o câncer. O presente trabalho teve como objetivo obter o perfil de separação de antocianinas em capimelefante por técnicas espectroscópicas e cromatográficas utilizando a metodologia do pH Único de forma a otimizar o processo de extração com foco às necessidades das rotinas industriais. As análises foram realizadas em amostras compostas de folhas de capim-elefante de coloração arroxeada. Utilizando dois reagentes, observa-se o alto teor de antocianinas obtido pela mistura de água e etanol que é semelhante a mistura usada para outros tipos de materiais (5,22mg/100g). A partir da identificação das substâncias pode-se inferir que a Pelargonidina 3-robinobiosídeo e a Malvidina são as antocianinas mais comuns nos extratos do genótipo de capim-elefante roxo. |
Palavras-Chave: |
Antocianidina; Composto bioativo; Planejamento de misturas. |
Thesagro: |
Antocianina. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1161013/1/Perfil-de-extracao-de-antocianinas-em-genotipo-de-capim-elefante-roxo.pdf
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Marc: |
LEADER 01764nam a2200205 a 4500 001 2161013 005 2024-01-18 008 2023 bl uuuu u00u1 u #d 100 1 $aNOGUEIRA, C. P. 245 $aPerfil de extração de antocianinas em genótipo de capim elefante roxo$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 12., 2023, Caxambu, MG. Anais. Piracicaba: Sociedade Brasileira de Melhoramento de Plantas$c2023 520 $aO capim-elefante roxo [Cenchrus purpureus (Schumach.) Morrone] tem a cor característica exibida pelos vegetais que possuem antocianinas que são um corante natural que apresenta efeito contra doenças cardiovasculares, neurodegenerativas e que pode prevenir o câncer. O presente trabalho teve como objetivo obter o perfil de separação de antocianinas em capimelefante por técnicas espectroscópicas e cromatográficas utilizando a metodologia do pH Único de forma a otimizar o processo de extração com foco às necessidades das rotinas industriais. As análises foram realizadas em amostras compostas de folhas de capim-elefante de coloração arroxeada. Utilizando dois reagentes, observa-se o alto teor de antocianinas obtido pela mistura de água e etanol que é semelhante a mistura usada para outros tipos de materiais (5,22mg/100g). A partir da identificação das substâncias pode-se inferir que a Pelargonidina 3-robinobiosídeo e a Malvidina são as antocianinas mais comuns nos extratos do genótipo de capim-elefante roxo. 650 $aAntocianina 653 $aAntocianidina 653 $aComposto bioativo 653 $aPlanejamento de misturas 700 1 $aADRIANO, L. H. C. 700 1 $aMOREIRA, O. B. de O. 700 1 $aOLIVEIRA, M. A. L. de 700 1 $aMACHADO, J. C.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Tabuleiros Costeiros. Para informações adicionais entre em contato com cpatc.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
19/12/2023 |
Data da última atualização: |
29/12/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
C - 0 |
Autoria: |
SANTOS, K. S.; COSTA, C.; BESSA, M. J.; TEIXEIRA, J. P.; SILVA, A. V. C. da; PADILHA, F. F.; DARIVA, C.; OLIVEIRA, M. B. P. P. |
Afiliação: |
KLEBSON SILVA SANTOS, ITP; CARLA COSTA, Portuguese National Institute of Health; MARIA JOÃO BESSA, Universidade do Porto; JOAO PAULO TEIXEIRA, UNIVERSIDADE DO PORTO; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC; FRANCINE FERREIRA PADILHA, ITP; CLÁUDIO DARIVA, ITP; MARIA BEATRIZ PINTO PRIOR OLIVEIRA, UNIVERSIDADE DO PORTO. |
Título: |
Azadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblastoid cells growth via cell cycle arrest, apoptosis induction, and DNA damage. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Exploration of Foods and Foodomics, v. 1, p. 130-142, 2023. |
DOI: |
https://doi.org/10.37349/eff.2023.00011 |
Idioma: |
Inglês |
Conteúdo: |
Aim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed
from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negative control (NC) to 83% after treatment with neem extracts (15 μg/mL). Neem extracts were also able to cause DNA strand breaks in TK6 cells. MenosAim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed
from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negati... Mostrar Tudo |
Thesagro: |
Conservação; Nim; Planta Medicinal. |
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
Marc: |
LEADER 02468naa a2200253 a 4500 001 2160010 005 2023-12-29 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.37349/eff.2023.00011$2DOI 100 1 $aSANTOS, K. S. 245 $aAzadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblastoid cells growth via cell cycle arrest, apoptosis induction, and DNA damage.$h[electronic resource] 260 $c2023 520 $aAim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was removed from the neem. The dried neem sample was used in a new extraction for obtaining the ethyl acetate extract (EAE). Total phenolic compounds (TPC) and total flavonoid contents (TFC) were determined by spectrophotometric methods. Lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) tests were used to evaluate the cytotoxicity in TK6 cells. The stop at G0/G1 cell cycle and inducing apoptosis in the TK6 cells were analyzed by flow cytometry. For deoxyribonucleic acid (DNA) damage evaluation, the alkaline comet test was used. Results: The higher TFC (65.50 mg/g of extract ± 1.17 mg/g of extract) and TPC (52.08 mg of extract ± 0.88 mg of extract) were obtained in EAE compared to HE that was obtained TFC of 14.61 mg/g of extract ± 0.60 mg/g of extract and TPC of 3.20 mg/g of extract ± 1.20 mg/g of extract. EAE was more significantly cytotoxic to TK6 cells than HE. The apoptosis induction was higher after exposure to 15.0 μg/mL of EAE (11.29%) in comparison to 15.0 μg/mL of HE (2.52%). The G0/G1 phase increased from 72% negative control (NC) to 83% after treatment with neem extracts (15 μg/mL). Neem extracts were also able to cause DNA strand breaks in TK6 cells. 650 $aConservação 650 $aNim 650 $aPlanta Medicinal 700 1 $aCOSTA, C. 700 1 $aBESSA, M. J. 700 1 $aTEIXEIRA, J. P. 700 1 $aSILVA, A. V. C. da 700 1 $aPADILHA, F. F. 700 1 $aDARIVA, C. 700 1 $aOLIVEIRA, M. B. P. P. 773 $tExploration of Foods and Foodomics$gv. 1, p. 130-142, 2023.
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