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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
04/09/2015 |
Data da última atualização: |
16/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FONSECA, F. C. de A.; FIRMINO, A. A. P.; MACEDO. L. L. P. de; COELHO, R. R.; SOUSA JÚNIOR, J. D. A. de; SILVA JUNIOR, O. B.; TOGAWA, R. C.; PAPPAS JUNIOR, G. J.; GÓIS, L. A. B. de; SILVA, M. C. M. da; SA, M. F. G. de. |
Afiliação: |
FERNANDO CAMPOS DE ASSIS FONSECA, UnB; ALEXANDRE AUGUSTO PEREIRA FIRMINO, UnB; LEONARDO LIMA PEPINO DE MACEDO, UnB; ROBERTA RAMOS COELHO; JOSÉ DIJAIR ANTONINO DE SOUSA JÚNIOR, UnB; ORZENIL BONFIM SILVA-JUNIOR, UnB; ROBERTO COITI TOGAWA, CENARGEN; GEORGIOS JOANNIS PAPPAS JUNIOR, Unb; LUIZ AVELAR BRANDÃO DE GÓIS, USINA TRUNFO, ALAGOAS; MARIA CRISTINA MATTAR DA SILVA, CENARGEN; MARIA FATIMA GROSSI DE SA, CENARGEN. |
Título: |
Sugarcane giant borer transcriptome analysis and identification of genes related to digestion. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Plos One, fev., 2015. |
DOI: |
10.1371/journal.pone.0118231 |
Idioma: |
Inglês |
Conteúdo: |
Sugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the Gen-Bank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST database for the species. Transcriptome analysis will certainly be useful to identify novel developmental genes, to better understand the insect?s biology and to guide the development of new strategies for insect-pest control. MenosSugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the Gen-Bank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST d... Mostrar Tudo |
Thesagro: |
Broca Gigante; Controle Biológico; Praga. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/129096/1/journal.pone.0118231.pdf
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Marc: |
LEADER 02579naa a2200289 a 4500 001 2023363 005 2023-03-16 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1371/journal.pone.0118231$2DOI 100 1 $aFONSECA, F. C. de A. 245 $aSugarcane giant borer transcriptome analysis and identification of genes related to digestion.$h[electronic resource] 260 $c2015 520 $aSugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the Gen-Bank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST database for the species. Transcriptome analysis will certainly be useful to identify novel developmental genes, to better understand the insect?s biology and to guide the development of new strategies for insect-pest control. 650 $aBroca Gigante 650 $aControle Biológico 650 $aPraga 700 1 $aFIRMINO, A. A. P. 700 1 $aMACEDO. L. L. P. de 700 1 $aCOELHO, R. R. 700 1 $aSOUSA JÚNIOR, J. D. A. de 700 1 $aSILVA JUNIOR, O. B. 700 1 $aTOGAWA, R. C. 700 1 $aPAPPAS JUNIOR, G. J. 700 1 $aGÓIS, L. A. B. de 700 1 $aSILVA, M. C. M. da 700 1 $aSA, M. F. G. de 773 $tPlos One, fev., 2015.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
02/07/1998 |
Data da última atualização: |
02/07/1998 |
Autoria: |
TORRES, A. C.; NAGATA, R. T.; FERL, R. J.; BEWICK, T. A.; CANTLIFFE, D. J. |
Afiliação: |
EMBRAPA-CNPH, Brasilia, DF. |
Título: |
Metodo in vitro para seleccionar plantas transgenicas resistentes al herbicida glifosato. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
In: ENCUENTRO LATINOAMERICANO DE BIOTECNOLOGIA VEGETAL, 3., 1998, La Habana, Cuba. Resumenes... La Habana: FAO, 1998. |
Páginas: |
p.335. |
Idioma: |
Espanhol |
Palavras-Chave: |
Lucerne; Planta daninha; Resistance. |
Thesagro: |
Alfafa; Cultura In Vitro; Erva Daninha; Herbicida; Medicago Sativa; Planta Transgênica; Resistência. |
Thesaurus NAL: |
alfalfa; in vitro culture; transgenic plants; weeds. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00960naa a2200337 a 4500 001 1761891 005 1998-07-02 008 1998 bl --- 0-- u #d 100 1 $aTORRES, A. C. 245 $aMetodo in vitro para seleccionar plantas transgenicas resistentes al herbicida glifosato. 260 $c1998 300 $ap.335. 650 $aalfalfa 650 $ain vitro culture 650 $atransgenic plants 650 $aweeds 650 $aAlfafa 650 $aCultura In Vitro 650 $aErva Daninha 650 $aHerbicida 650 $aMedicago Sativa 650 $aPlanta Transgênica 650 $aResistência 653 $aLucerne 653 $aPlanta daninha 653 $aResistance 700 1 $aNAGATA, R. T. 700 1 $aFERL, R. J. 700 1 $aBEWICK, T. A. 700 1 $aCANTLIFFE, D. J. 773 $tIn: ENCUENTRO LATINOAMERICANO DE BIOTECNOLOGIA VEGETAL, 3., 1998, La Habana, Cuba. Resumenes... La Habana: FAO, 1998.
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