Data/hora: 24/06/2024 00:40:36

Biblioteca(s): | Embrapa Gado de Corte.
Data corrente: | 21/03/2019
Data da última atualização: | 21/03/2019
Autoria: | OLIVEIRA, A. L. B. de; MACEDO, G. C. de; ROSINHA, G. M. S.; MELGAREJO, J. L.; ALVES, A. G. L.; BARRETO, W. T. G.; SANTOS, F. M.; CAMPOS, J. B. V.; HERRERA, H. M.; OLIVEIRA, C. E. de.
Afiliação: | Ana Laura Bello de Oliveira, Universidade Católica Dom Bosco - UCDB; Gabriel Carvalho de Macedo, Universidade Católica Dom Bosco - UCDB; GRACIA MARIA SOARES ROSINHA, CNPGC; Jhessyca Leal Melgarejo, Universidade Católica Dom Bosco - UCDB; Andreza Gabriela Leão Alves, Universidade Católica Dom Bosco - UCDB; Wanessa Teixeira Gomes Barreto, Universidade Católica Dom Bosco - UCDB; Filipe Martins Santos, Universidade Católica Dom Bosco - UCDB; João Bosco Vilela Campos, Universidade Católica Dom Bosco - UCDB; Heitor Miraglia Herrera, Universidade Católica Dom Bosco - UCDB; Carina Elisei de Oliveira, Universidade Católica Dom Bosco - UCDB.
Título: | Detection of Brucella spp. in dogs at Pantanal wetlands.
Ano de publicação: | 2019
Fonte/Imprenta: | Brazilian Journal of Microbiology, v. 50, n. 1, p. 307-312, 2019.
Idioma: | Inglês
Conteúdo: | Canine brucellosis is an infectious disease that produces reproductive disease in both males and females. Although Brucella canis is more common, the infection by Brucella abortus is more frequent in dogs sharing habitats with livestock and wild animals.We decided to investigate the role of dogs in the maintenance of Brucella spp. in the Pantanal wetland. Serum and whole blood samples were collected from 167 dogs. To detect antibodies against B. abortus and B. canis, buffered acidified plate antigen (BAPA) and agar gel immunodiffusion (AGID) tests were performed. To detect Brucella spp., B. abortus and B. canis DNA, PCR was performed using the bcsp31, BruAb2_0168, and BR00953 genes, respectively. To confirm the PCR results, three bcsp31 PCR products were sequenced and compared with sequences deposited in GenBank. The seropositivity rates of 7.8% and 9% were observed for the AGID and BAPA tests, respectively. Positivity rates of 45.5% and 10.8% were observed when testing bcsp31 and BruAb2_0168, respectively, while there was no positivity for BR00953. The sequenced products had 110 base pairs that aligned with 100% identity to B. abortus, B. canis, and B. suis. Considering our results, dogs may be acting as maintenance hosts of Brucella spp. in the Pantanal region.
Palavras-Chave: | Molecular tests; Reservoir host.
Thesaurus Nal: | Pantanal; Serology.
Categoria do assunto: | --
Registro original: | Embrapa Gado de Corte (CNPGC)
Exemplares: | CNPGC - (17333-1) - UPC - AP - DD -  -  -  - DIS