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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
05/12/2022 |
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Data da última atualização: |
13/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PATROCINIO, T. A.; FERNANDES, C. A. de C.; MACEDO, G. C.; SILVA, A. D. da; BARBOSA, N. R. R.; QUINTAO, C. C. R.; RIBEIRO, M. E. da S.; CAMARGO, L. S. de A. |
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Afiliação: |
TAIS APARECIDA PATROCINIO, UNIFENAS; CARLOS ANTÔNIO DE CARVALHO FERNANDES, UNIFENAS; GILSON COSTA MACEDO, Universidade Federal de Juiz de Fora; ADILSON DAVID DA SILVA, Universidade Federal de Juiz de Fora; NADIA REZENDE RAPOSO BARBOSA, Universidade Federal de Juiz de Fora; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; MARCELA EDUARDA DA SILVA RIBEIRO, UNIFENAS; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
RA33, an analogue of resveratrol, improves the development of in vitro-fertilized bovine embryos. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Zygote, v. 30, n. 6, p. 891-894, 2022. |
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DOI: |
https://doi.org/10.1017/S0967199422000430 |
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Idioma: |
Inglês |
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Conteúdo: |
Oxidative stress is an undesirable effect of in vitro culture, which requires antioxidant supplementation. This study investigated the analogue of resveratrol (RA33) as an alternative to resveratrol, an antioxidant molecule, for the in vitro culture of in vitro-fertilized bovine embryos. The effect of different concentrations of RA33 on embryo development was evaluated and a comparison between RA33 and resveratrol was performed. The cleavage rate was higher (P < 0.05) with 2.5 μM (69.0 ± 4.4%) than at 0, 0.1 or 0.5 uM RA33 (62.1 ± 2.0%, 60.7 ± 5.9% and 56.7 ± 5.8%, respectively). The blastocyst rates on days 7 and 8 post-fertilization with 2.5 μM RA33 (19.4 ± 3.3% and 24.6 ± 3.3%, respectively) were higher (P < 0.05) than for 0 uM (12.4 ± 2.5% and 15.2±2.5%, respectively). When 2.5 uM RA33 was compared with 0.5 uM resveratrol, similar (P > 0.05) cleavage and blastocyst rates were found between them, but the cleavage rate was higher (P < 0.05) in the control (80.8 ± 3.4%) than for the resveratrol treatment (76.4 ± 3.6%). The numbers of apoptotic cells and the apoptotic index were lower (P < 0.05) with RA33 (6.5 ± 0.6 cells and 6.4 ± 0.7%, respectively) and resveratrol (5 ± 0.8 cells and 5.5 ± 1.0%, respectively) than in the control group (9.8 ± 1.2 cells and 8.9 ± 1.1%, respectively). In conclusion, RA33 can enhance the preimplantation development of in vitro-fertilized bovine embryos and be an alternative to resveratrol in embryo culture medium. |
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Palavras-Chave: |
Apoptose; Fertilização in vitro. |
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Thesagro: |
Antioxidante; Bovino; Cultura de Embrião; Embrião Animal; Fertilidade Animal. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02417naa a2200301 a 4500
001 2149208
005 2023-01-13
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S0967199422000430$2DOI
100 1 $aPATROCINIO, T. A.
245 $aRA33, an analogue of resveratrol, improves the development of in vitro-fertilized bovine embryos.$h[electronic resource]
260 $c2022
520 $aOxidative stress is an undesirable effect of in vitro culture, which requires antioxidant supplementation. This study investigated the analogue of resveratrol (RA33) as an alternative to resveratrol, an antioxidant molecule, for the in vitro culture of in vitro-fertilized bovine embryos. The effect of different concentrations of RA33 on embryo development was evaluated and a comparison between RA33 and resveratrol was performed. The cleavage rate was higher (P < 0.05) with 2.5 μM (69.0 ± 4.4%) than at 0, 0.1 or 0.5 uM RA33 (62.1 ± 2.0%, 60.7 ± 5.9% and 56.7 ± 5.8%, respectively). The blastocyst rates on days 7 and 8 post-fertilization with 2.5 μM RA33 (19.4 ± 3.3% and 24.6 ± 3.3%, respectively) were higher (P < 0.05) than for 0 uM (12.4 ± 2.5% and 15.2±2.5%, respectively). When 2.5 uM RA33 was compared with 0.5 uM resveratrol, similar (P > 0.05) cleavage and blastocyst rates were found between them, but the cleavage rate was higher (P < 0.05) in the control (80.8 ± 3.4%) than for the resveratrol treatment (76.4 ± 3.6%). The numbers of apoptotic cells and the apoptotic index were lower (P < 0.05) with RA33 (6.5 ± 0.6 cells and 6.4 ± 0.7%, respectively) and resveratrol (5 ± 0.8 cells and 5.5 ± 1.0%, respectively) than in the control group (9.8 ± 1.2 cells and 8.9 ± 1.1%, respectively). In conclusion, RA33 can enhance the preimplantation development of in vitro-fertilized bovine embryos and be an alternative to resveratrol in embryo culture medium.
650 $aAntioxidante
650 $aBovino
650 $aCultura de Embrião
650 $aEmbrião Animal
650 $aFertilidade Animal
653 $aApoptose
653 $aFertilização in vitro
700 1 $aFERNANDES, C. A. de C.
700 1 $aMACEDO, G. C.
700 1 $aSILVA, A. D. da
700 1 $aBARBOSA, N. R. R.
700 1 $aQUINTAO, C. C. R.
700 1 $aRIBEIRO, M. E. da S.
700 1 $aCAMARGO, L. S. de A.
773 $tZygote$gv. 30, n. 6, p. 891-894, 2022.
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Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
19/02/2016 |
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Data da última atualização: |
15/04/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BONONI, L.; VASCONCELLOS, R. L. F.; TAKETANI, R. G.; VILELA, E. S. D.; MOITINHO, M. A.; MELO, I. S. de. |
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Afiliação: |
L. BONONI, ESALQ/USP; R. L. F. VASCONCELLOS; R. G. TAKETANI, Bolsista FAPESP; E. S. D. VILELA; M. A. MOITINHO, ESALQ/USP; ITAMAR SOARES DE MELO, CNPMA. |
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Título: |
Change in enzymatic activity of microbial communities on the seasonal differences in soils the of biomes caatinga and atlantic forest. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 0973-1. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The average global temperature has increased and will continue increasing, with forecast of up to 5.8° C by the end of this century as a result of the increase in the concentration of greenhouse gases in the atmosphere. Due to these increased temperature, some biomes may introduce features of arid and semi-arid environments in the coming years. The patterns of water availability in the soil can select groups of microorganisms with physiological strategies to tolerate these seasonal changes. Nutrient availability for plants becomes limited when the aqueous part of the soil is low in the dry period. On the other hand, in irrigated soils the concentration of nutrients available associated with mineralization increases rapidly. Environmental indicators such as seasonal variations in the enzymatic activity, the abundance of microorganisms and soil physical and chemical characteristics, are useful to measure and analyze the change of the functionality soil microbiota. This work was aimed to study the seasonal changes of enzymatic activity (dehydrogenase, acid phosphatase and alkaline, arylsulfatase, phytase, cellulase, amylase and urease) of microbial communities in soils of Caatinga and Atlantic Forest. The soils were collected in Bertioga-SP, Cananéia-SP, Juazeiro-BA and Petrolina-PE. The samples of soil were sifted and separated into 18 glass bottles of 200 mL containing 50 g of each soil. The bottles were incubated at 28° C varying moisture in 60% and 30% every 14 days corresponding to three seasonal cycles. The sequencing of 16S ribosomal RNA gene (rRNA) of samples was performed using the Ion Sequencing Kit 400 (Life Technologies). The dynamics of the bacterial communities had influences of seasonal changes, especially when compared the biogeographic patterns. The effect of moisture was significantly different (p < 0.05) only to the activity of alkaline phosphatase and phytase enzymes. The activity of all enzymes, except phytase, was important to discriminate the soils between Caatinga and Atlantic Forest biomes. Seasonality had an effect on the enzymatic activity that was related with the functional change in the soil microbiota. MenosAbstract: The average global temperature has increased and will continue increasing, with forecast of up to 5.8° C by the end of this century as a result of the increase in the concentration of greenhouse gases in the atmosphere. Due to these increased temperature, some biomes may introduce features of arid and semi-arid environments in the coming years. The patterns of water availability in the soil can select groups of microorganisms with physiological strategies to tolerate these seasonal changes. Nutrient availability for plants becomes limited when the aqueous part of the soil is low in the dry period. On the other hand, in irrigated soils the concentration of nutrients available associated with mineralization increases rapidly. Environmental indicators such as seasonal variations in the enzymatic activity, the abundance of microorganisms and soil physical and chemical characteristics, are useful to measure and analyze the change of the functionality soil microbiota. This work was aimed to study the seasonal changes of enzymatic activity (dehydrogenase, acid phosphatase and alkaline, arylsulfatase, phytase, cellulase, amylase and urease) of microbial communities in soils of Caatinga and Atlantic Forest. The soils were collected in Bertioga-SP, Cananéia-SP, Juazeiro-BA and Petrolina-PE. The samples of soil were sifted and separated into 18 glass bottles of 200 mL containing 50 g of each soil. The bottles were incubated at 28° C varying moisture in 60% and 30% every 14 da... Mostrar Tudo |
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Palavras-Chave: |
Arid environments; Seasonality; Soil microbiota. |
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Thesagro: |
Caatinga. |
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Thesaurus NAL: |
Dry environmental conditions; Enzyme activity; Soil microorganisms. |
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Categoria do assunto: |
V Taxonomia de Organismos |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139543/1/2015RA-018.pdf
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Marc: |
LEADER 03090nam a2200253 a 4500
001 2037853
005 2016-04-15
008 2015 bl uuuu u00u1 u #d
100 1 $aBONONI, L.
245 $aChange in enzymatic activity of microbial communities on the seasonal differences in soils the of biomes caatinga and atlantic forest.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 0973-1.$c2015
520 $aAbstract: The average global temperature has increased and will continue increasing, with forecast of up to 5.8° C by the end of this century as a result of the increase in the concentration of greenhouse gases in the atmosphere. Due to these increased temperature, some biomes may introduce features of arid and semi-arid environments in the coming years. The patterns of water availability in the soil can select groups of microorganisms with physiological strategies to tolerate these seasonal changes. Nutrient availability for plants becomes limited when the aqueous part of the soil is low in the dry period. On the other hand, in irrigated soils the concentration of nutrients available associated with mineralization increases rapidly. Environmental indicators such as seasonal variations in the enzymatic activity, the abundance of microorganisms and soil physical and chemical characteristics, are useful to measure and analyze the change of the functionality soil microbiota. This work was aimed to study the seasonal changes of enzymatic activity (dehydrogenase, acid phosphatase and alkaline, arylsulfatase, phytase, cellulase, amylase and urease) of microbial communities in soils of Caatinga and Atlantic Forest. The soils were collected in Bertioga-SP, Cananéia-SP, Juazeiro-BA and Petrolina-PE. The samples of soil were sifted and separated into 18 glass bottles of 200 mL containing 50 g of each soil. The bottles were incubated at 28° C varying moisture in 60% and 30% every 14 days corresponding to three seasonal cycles. The sequencing of 16S ribosomal RNA gene (rRNA) of samples was performed using the Ion Sequencing Kit 400 (Life Technologies). The dynamics of the bacterial communities had influences of seasonal changes, especially when compared the biogeographic patterns. The effect of moisture was significantly different (p < 0.05) only to the activity of alkaline phosphatase and phytase enzymes. The activity of all enzymes, except phytase, was important to discriminate the soils between Caatinga and Atlantic Forest biomes. Seasonality had an effect on the enzymatic activity that was related with the functional change in the soil microbiota.
650 $aDry environmental conditions
650 $aEnzyme activity
650 $aSoil microorganisms
650 $aCaatinga
653 $aArid environments
653 $aSeasonality
653 $aSoil microbiota
700 1 $aVASCONCELLOS, R. L. F.
700 1 $aTAKETANI, R. G.
700 1 $aVILELA, E. S. D.
700 1 $aMOITINHO, M. A.
700 1 $aMELO, I. S. de
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