Data/hora: 23/06/2024 13:07:26

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 09/09/1993
Data da última atualização: | 09/09/1993
Autoria: | MACDONALD, H. M.; EVANS, R.; SPENCER, W. J.
Afiliação: | Reading Scientific Services Ltd, The Lord Zuckerman Research Centre, The University, PO Box 234, Whiteknights, Reading RG6 2LA, UK.
Título: | Purification and properties of the majo pactinesterases in lemon fruits (Citrus limon).
Ano de publicação: | 1993
Fonte/Imprenta: | Journal of the Science of Food and Agriculture. v.62, n.2, p.163-168, 1993.
ISSN: | 0022-5142
Idioma: | Inglês
Conteúdo: | Pectinesterase from lemon was separated into seven fracions by chromatography on CM-Sephadex C-50. Purification was hindered by the presence of pectin, for which the pectinesterase had a high affinity. Two majo pectinesterases were purified: one was located solely in the peel and the other in the endocarp. They possessed similar molecular weights (35 kDa, respectively) and high isoelectric points (> 11, and c 9). They required the presence of cations for optimal activity, the peel pectinesterase requring a higher concentration of cations than the endocarp enzyme. Bothe enzymes were completely inactivated at temperatures above 88 degree C. Although neith enzyme destabilised the cloud of lemon juice or of comminuted lemon beverages, they caused cloud destabilisation of orange juice.
Palavras-Chave: | Lemon; Pactinesterase; purification.
Thesaurus Nal: | Citrus limon.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (2206-1) - ADC - AP - -- - 664.005 -  - 664.005 - DIS