| |
|
|
| Registros recuperados : 33 | |
| 5. |  | RIBEIRO, R. C.; LEMOS, W. P.; SOUZA, M. T. de; COSTA, M. B. de O. Insetos com potencial de causar danos a plantios de helicônias (Heliconiaceae) no Município de Castanhal, PA. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA UFRA, 5.; SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL (AVALIAÇÃO - 2007), 11., 2008, Belém, PA. Mudanças climáticas globais: sustentabilidade na Amazônia: anais. Belém, PA: UFRA: Embrapa Amazônia Oriental, 2008.| Biblioteca(s): Embrapa Amazônia Oriental. |
|    |
| 7. | | RIBEIRO, R. C.; LEMOS, W. P.; SOUZA, M. T.; COSTA, M. B. de O. Insetos associados a bastão-do-imperador, Etlingera elatior (Zingiberaceae) no Município de Castanhal. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA UFRA, 5.; SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL (AVALIAÇÃO - 2007), 11., 2008, Belém, PA. Mudanças climáticas globais: sustentabilidade na Amazônia: anais. Belém, PA: UFRA: Embrapa Amazônia Oriental, 2008.| Biblioteca(s): Embrapa Amazônia Oriental. |
| |
| 11. | | LEMOS, W. P.; COSTA, M. B. de O.; THOMAZ, M. A. A.; ARAÚJO, P. P. M. de; ATAÍDE, I. Ocorrência de Deois incompleta (Hemiptera: Cercopidae) em Brachiaria brizantha no município de Uruará, PA. In: SEMANA DE INTEGRAÇÃO DAS CIÊNCIAS AGRÁRIAS, 8., 2008, Altamira. Anais... Altamira, PA: UFPA, 2008. p. 351-358.| Biblioteca(s): Embrapa Amazônia Oriental. |
| |
| 14. | | SOUZA, V. B. de; CARVALHO, A. J. de; SILVA, K. J. D.; ROCHA, M. de M.; PEREIRA FILHO, I. A.; BRITO, O. G.; SILVA, M. B. de O. Adaptabilidade e estabilidade produtiva de linhagens-elite e cultivares de feijão-caupi de portes ereto e semiereto em ambientes de Mato Grosso e Minas Gerais. In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 4., 2016, Sorriso. Feijão-caupi: avanços e desafios tecnológicos e de mercado: resumos. Brasília, DF: Embrapa, 2016. p. 132.| Biblioteca(s): Embrapa Milho e Sorgo. |
| |
| 15. | | BARBOSA, E. da S.; SILVA, M. B. de O.; ALVES, J. B.; BARBOSA, J. A. E.; SOUZA, T. L. P. O. de; CARVALHO, A. J. de. Fitossociologia de plantas daninhas em cultivo de feijão-comum BRS FC402 em diferentes densidades de semeadura. In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 13., 2021, Goiânia. Conectividade tecnológica, intensificação sustentável: resumos. Brasília, DF: Embrapa; Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2021. p. 237. Evento online.| Biblioteca(s): Embrapa Arroz e Feijão. |
| |
| 16. | | PARAHYBA, R. da B. V.; LUCENA, A. M. A. de; NETO, M. B. de O.; SANTOS, J. C. P. dos; SILVA, M. S. L. da. Caracterização dos solos da reserva biológica de Serra Negra - PE: 1º etapa - Porção Sudeste da Serra. In: JORNADA DE ENSINO, PESQUISA E EXTENSÃO DA UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO, 8., 24-31 out. 2008. Evoluçaõ e Diversidade. Recife, PE. [Anais...] Recife, PE: Universidade Federal de Pernambuco, 2008. 2 p. il.| Biblioteca(s): Embrapa Solos. |
| |
| 17. | | SOUZA, V. B. de; CARVALHO, A. J. de; BRITO, O. G.; SILVA, M. B. de O.; ROCHA, M. de M.; SILVA, K. J. D.; PEREIRA FILHO, I. A. Comprimento de vagem e índice de grãos de genótipos de feijão-caupi de porte semiprostrado em ambientes de Mato Grosso e Minas Gerais. In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 4., 2016, Sorriso. Feijão-caupi: avanços e desafios tecnológicos e de mercado: resumos. Brasília, DF: Embrapa, 2016. p. 154.| Biblioteca(s): Embrapa Milho e Sorgo. |
| |
| 18. | | SILVA, M. B. de O.; CARVALHO, A. J. de; ROCHA, M. de M.; BATISTA, P. S. C.; SANTOS JÚNIOR, P. V.; OLIVEIRA, S. M. de. Desempenho agronómico de genótipos de feijão-caupi. Revista de Ciências Agrárias, v. 41, n. 4, p. 1059-1066, 2018.| Biblioteca(s): Embrapa Meio-Norte. |
| |
| 19. | | RIBEIRO, F. N.; CHAVES, V. C.; MENDONÇA. C. E. S.; SILVA, M. S. L. da; CUNHA, T. J. F.; MOREIRA, M. M.; NETO, M. B. de O. Estudo de caso: impacto de barragem subterrânea no agroecossistema rural e na vida do agricultor. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 32., 2009, Fortaleza. O solo e a produção de bioenergia: perspectivas e desafios. Fortaleza: UFC: SBCS, 2009. 1 CD-ROM.| Biblioteca(s): Embrapa Semiárido. |
| |
| 20. | | FIRMINO, A.; ABREU, H. dos S.; NASCIMENTO, A. M. do; PEREIRA, R. P. W.; MONTEIRO, M. B. de O.; SOUSA, E. L. de; MAÊDA, J. M. Lignification of Solanum gilo Raddi induced by calcium nutrition. Floresta e Ambiente, Rio de Janeiro, v. 10, n. 2, p. 105-107, ago./dez. 2003.| Biblioteca(s): Embrapa Florestas. |
| |
| Registros recuperados : 33 | |
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
|
Data corrente: |
18/04/2023 |
|
Data da última atualização: |
25/05/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
VASQUEZ, D. D. N.; PINHEIRO, D. H.; TEIXEIRA, L. A.; MOREIRA-PINTO, C. E.; MACEDO, L. L. P. de; SALLES-FILHO, A. L. O.; SILVA, M. C. M. da; LOURENCO, I. T.; MORGANTE, C. V.; SILVA, L. P. da; SA, M. F. G. de. |
|
Afiliação: |
DANIEL D. N. VASQUEZ, Catholic University of Brasília; DANIELE H. PINHEIRO; LAYS A. TEIXEIRA, Catholic University of Brasília; CLIDIA E. MOREIRA-PINTO; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; ALVARO L. O. SALLES-FILHO, Federal University of Paraná; MARIA CRISTINA MATTAR DA SILVA, Cenargen; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; LUCIANO PAULINO DA SILVA, Cenargen; MARIA FATIMA GROSSI DE SA, Cenargen. |
|
Título: |
Simultaneous silencing of juvenile hormone metabolism genes through RNAi interrupts metamorphosis in the cotton boll weevil. |
|
Ano de publicação: |
2023 |
|
Fonte/Imprenta: |
Frontiers in Molecular Biosciences, v. 10, 2023. |
|
DOI: |
https://doi.org/10.3389/fmolb.2023.1073721 |
|
Idioma: |
Inglês |
|
Notas: |
Na publicação: Leonardo L. P. Macedo; Maria C. M. Silva; Isabela T. Lourenço-Tessutti; Luciano P. Silva; Maria F. Grossi-de-Sa. |
|
Conteúdo: |
The cotton boll weevil (CBW) (Anthonomus grandis) is one of the major insect pests of cotton in Brazil. Currently, CBW control is mainly achieved by insecticide application, which is costly and insufficient to ensure effective crop protection. RNA interference (RNAi) has been used in gene function analysis and the development of insect control methods. However, some insect species respond poorly to RNAi, limiting the widespread application of this approach. Therefore, nanoparticles have been explored as an option to increase RNAi efficiency in recalcitrant insects. Herein, we investigated the potential of chitosan?tripolyphosphate (CS-TPP) and polyethylenimine (PEI) nanoparticles as a dsRNA carrier system to improve RNAi efficiency in the CBW. Different formulations of the nanoparticles with dsRNAs targeting genes associated with juvenile hormone metabolism, such as juvenile hormone diol kinase (JHDK), juvenile hormone epoxide hydrolase (JHEH), and methyl farnesoate hydrolase (MFE), were tested. The formulations were delivered to CBW larvae through injection (0.05?2 ?g), and the expression of the target genes was evaluated using RT-qPCR. PEI nanoparticles increased targeted gene silencing compared with naked dsRNAs (up to 80%), whereas CS-TPP-dsRNA nanoparticles decreased gene silencing (0%?20%) or led to the same level of gene silencing as the naked dsRNAs (up to 50%). We next evaluated the effects of targeting a single gene orsimultaneously targeting two genes via the injection of naked dsRNAs or dsRNAs complexed with PEI (500 ng) on CBW survival and phenotypes. Overall, the gene expression analysis showed that the treatments with PEI targeting either a single gene or multiple genes induced greater gene silencing than naked dsRNA (~60%). In addition, the injection of dsJHEH/JHDK, either naked or complexed with PEI, significantly affected CBW survival (18% for PEI nanoparticles and 47% for naked dsRNA) and metamorphosis. Phenotypic alterations, such as uncompleted pupation or malformed pupae, suggested that JHEH and JHDK are involved in developmental regulation. Moreover, CBW larvae treated with dsJHEH/JHDK + PEI (1,000 ng/g) exhibited significantly lower survival rate (55%) than those that MenosThe cotton boll weevil (CBW) (Anthonomus grandis) is one of the major insect pests of cotton in Brazil. Currently, CBW control is mainly achieved by insecticide application, which is costly and insufficient to ensure effective crop protection. RNA interference (RNAi) has been used in gene function analysis and the development of insect control methods. However, some insect species respond poorly to RNAi, limiting the widespread application of this approach. Therefore, nanoparticles have been explored as an option to increase RNAi efficiency in recalcitrant insects. Herein, we investigated the potential of chitosan?tripolyphosphate (CS-TPP) and polyethylenimine (PEI) nanoparticles as a dsRNA carrier system to improve RNAi efficiency in the CBW. Different formulations of the nanoparticles with dsRNAs targeting genes associated with juvenile hormone metabolism, such as juvenile hormone diol kinase (JHDK), juvenile hormone epoxide hydrolase (JHEH), and methyl farnesoate hydrolase (MFE), were tested. The formulations were delivered to CBW larvae through injection (0.05?2 ?g), and the expression of the target genes was evaluated using RT-qPCR. PEI nanoparticles increased targeted gene silencing compared with naked dsRNAs (up to 80%), whereas CS-TPP-dsRNA nanoparticles decreased gene silencing (0%?20%) or led to the same level of gene silencing as the naked dsRNAs (up to 50%). We next evaluated the effects of targeting a single gene orsimultaneously targeting two genes via the inj... Mostrar Tudo |
|
Palavras-Chave: |
Bicudo do algodoeiro; Juvenile diol kinase; Juvenile hormone epoxide hydrolase; Polyethylenimine; Pragas do algodoeiro. |
|
Thesagro: |
Algodão; Anthonomus Grandis; Inseto; Praga. |
|
Thesaurus NAL: |
Chitosan; Cotton; Insect control; Insect pests. |
|
Categoria do assunto: |
--
O Insetos e Entomologia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1153217/1/fmolb-10-1073721.pdf
|
|
Marc: |
LEADER 03612naa a2200421 a 4500
001 2153271
005 2023-05-25
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fmolb.2023.1073721$2DOI
100 1 $aVASQUEZ, D. D. N.
245 $aSimultaneous silencing of juvenile hormone metabolism genes through RNAi interrupts metamorphosis in the cotton boll weevil.$h[electronic resource]
260 $c2023
500 $aNa publicação: Leonardo L. P. Macedo; Maria C. M. Silva; Isabela T. Lourenço-Tessutti; Luciano P. Silva; Maria F. Grossi-de-Sa.
520 $aThe cotton boll weevil (CBW) (Anthonomus grandis) is one of the major insect pests of cotton in Brazil. Currently, CBW control is mainly achieved by insecticide application, which is costly and insufficient to ensure effective crop protection. RNA interference (RNAi) has been used in gene function analysis and the development of insect control methods. However, some insect species respond poorly to RNAi, limiting the widespread application of this approach. Therefore, nanoparticles have been explored as an option to increase RNAi efficiency in recalcitrant insects. Herein, we investigated the potential of chitosan?tripolyphosphate (CS-TPP) and polyethylenimine (PEI) nanoparticles as a dsRNA carrier system to improve RNAi efficiency in the CBW. Different formulations of the nanoparticles with dsRNAs targeting genes associated with juvenile hormone metabolism, such as juvenile hormone diol kinase (JHDK), juvenile hormone epoxide hydrolase (JHEH), and methyl farnesoate hydrolase (MFE), were tested. The formulations were delivered to CBW larvae through injection (0.05?2 ?g), and the expression of the target genes was evaluated using RT-qPCR. PEI nanoparticles increased targeted gene silencing compared with naked dsRNAs (up to 80%), whereas CS-TPP-dsRNA nanoparticles decreased gene silencing (0%?20%) or led to the same level of gene silencing as the naked dsRNAs (up to 50%). We next evaluated the effects of targeting a single gene orsimultaneously targeting two genes via the injection of naked dsRNAs or dsRNAs complexed with PEI (500 ng) on CBW survival and phenotypes. Overall, the gene expression analysis showed that the treatments with PEI targeting either a single gene or multiple genes induced greater gene silencing than naked dsRNA (~60%). In addition, the injection of dsJHEH/JHDK, either naked or complexed with PEI, significantly affected CBW survival (18% for PEI nanoparticles and 47% for naked dsRNA) and metamorphosis. Phenotypic alterations, such as uncompleted pupation or malformed pupae, suggested that JHEH and JHDK are involved in developmental regulation. Moreover, CBW larvae treated with dsJHEH/JHDK + PEI (1,000 ng/g) exhibited significantly lower survival rate (55%) than those that
650 $aChitosan
650 $aCotton
650 $aInsect control
650 $aInsect pests
650 $aAlgodão
650 $aAnthonomus Grandis
650 $aInseto
650 $aPraga
653 $aBicudo do algodoeiro
653 $aJuvenile diol kinase
653 $aJuvenile hormone epoxide hydrolase
653 $aPolyethylenimine
653 $aPragas do algodoeiro
700 1 $aPINHEIRO, D. H.
700 1 $aTEIXEIRA, L. A.
700 1 $aMOREIRA-PINTO, C. E.
700 1 $aMACEDO, L. L. P. de
700 1 $aSALLES-FILHO, A. L. O.
700 1 $aSILVA, M. C. M. da
700 1 $aLOURENCO, I. T.
700 1 $aMORGANTE, C. V.
700 1 $aSILVA, L. P. da
700 1 $aSA, M. F. G. de
773 $tFrontiers in Molecular Biosciences$gv. 10, 2023.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Semiárido (CPATSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
