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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
26/11/2009 |
Data da última atualização: |
15/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MEO, S. C.; FERREIRA, C. R.; PERECIN, F.; SARAIVA, N. Z.; TETZNER, T. A. D.; YAMAZAKI, W.; LEA, C. L. V.; MEIRELLES, F. V.; GARCIA, J. M. |
Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; CHRISTINA RAMIRES FERREIRA, USP; FELIPE PERECIN, USP; NAIARA ZOCCAL SARAIVA, UNESP; TATIANE ALMEIDA DRUMMOND TETZNER, UNESP; WALT YAMAZAKI, UNESP; CLÁUDIA LIMA VERDE LEA, USP; FLÁVIO VIEIRA MEIRELLES, USP; JOAQUIM MANSANO GARCIA, UNESP. |
Título: |
Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Animal Reproduction Science, v. 116, n. 3-4, p. 381-385, dec. 2009. |
DOI: |
https://doi.org/10.1016/j.anireprosci.2009.02.020 |
Idioma: |
Inglês |
Conteúdo: |
Ooplasmic factors drive nuclear organization after fertilization and are also important for re-programming in nuclear transfer procedures, in which artificial activation is essential for reconstructed embryos to progress in development. The present research evaluated the effect of pronuclear transfer (PT) between zygotes parthenogenetically activated with ionomycin followed by strontium (S) or 6-DMAP (D) on early embryonic development. PT was performed in the same zygote to obtain embryos in control groups (S-PT and D-PT) and between zygotes activated with S and D to achieve embryos with differentially activated cytoplasm (C) and nucleus (N) (SCDN and DCSN). PT procedure did not affect cleavage and blastocyst rates, respectively, in PT control groups compared to non-manipulated control (S-PT: 73.6% and 7.3% compared with S-Control: 77.9% and 7.8%; and D-PT: 73.3% and 31.7% compared with D-Control: 83.1% and 41.5%). Cleavage, eight-cell, and blastocyst rates, respectively, were similar between SCDN (76.5%, 36.4%, and 6.8%) and DCSN (69.5%, 25.0%, and 4.9%) embryos. Developmental rates in SCDN were similar to S-PT, but inferior to D-PT. Developmental arrest up to eight-cell stage was greater in SCDN and DCSN than in S-PT and D-PT. In conclusion, karyoplast exchange between parthenogenetic zygotes activated with strontium and 6-DMAP can lead to nuclear?cytoplasmic incompatibilities and affect embryonic development to the eight-cell and blastocyst stages. |
Palavras-Chave: |
Artificial activation; Bovine; Embryonic development; Pronuclear transfer. |
Thesaurus Nal: |
beef cattle. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02365naa a2200289 a 4500 001 1576277 005 2023-03-15 008 2009 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.anireprosci.2009.02.020$2DOI 100 1 $aMEO, S. C. 245 $aKaryoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle.$h[electronic resource] 260 $c2009 520 $aOoplasmic factors drive nuclear organization after fertilization and are also important for re-programming in nuclear transfer procedures, in which artificial activation is essential for reconstructed embryos to progress in development. The present research evaluated the effect of pronuclear transfer (PT) between zygotes parthenogenetically activated with ionomycin followed by strontium (S) or 6-DMAP (D) on early embryonic development. PT was performed in the same zygote to obtain embryos in control groups (S-PT and D-PT) and between zygotes activated with S and D to achieve embryos with differentially activated cytoplasm (C) and nucleus (N) (SCDN and DCSN). PT procedure did not affect cleavage and blastocyst rates, respectively, in PT control groups compared to non-manipulated control (S-PT: 73.6% and 7.3% compared with S-Control: 77.9% and 7.8%; and D-PT: 73.3% and 31.7% compared with D-Control: 83.1% and 41.5%). Cleavage, eight-cell, and blastocyst rates, respectively, were similar between SCDN (76.5%, 36.4%, and 6.8%) and DCSN (69.5%, 25.0%, and 4.9%) embryos. Developmental rates in SCDN were similar to S-PT, but inferior to D-PT. Developmental arrest up to eight-cell stage was greater in SCDN and DCSN than in S-PT and D-PT. In conclusion, karyoplast exchange between parthenogenetic zygotes activated with strontium and 6-DMAP can lead to nuclear?cytoplasmic incompatibilities and affect embryonic development to the eight-cell and blastocyst stages. 650 $abeef cattle 653 $aArtificial activation 653 $aBovine 653 $aEmbryonic development 653 $aPronuclear transfer 700 1 $aFERREIRA, C. R. 700 1 $aPERECIN, F. 700 1 $aSARAIVA, N. Z. 700 1 $aTETZNER, T. A. D. 700 1 $aYAMAZAKI, W. 700 1 $aLEA, C. L. V. 700 1 $aMEIRELLES, F. V. 700 1 $aGARCIA, J. M. 773 $tAnimal Reproduction Science$gv. 116, n. 3-4, p. 381-385, dec. 2009.
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Embrapa Pecuária Sudeste (CPPSE) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Meio-Norte. Para informações adicionais entre em contato com cpamn.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Meio-Norte. |
Data corrente: |
25/02/2010 |
Data da última atualização: |
25/02/2010 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
ARAÚJO, A. M. de; COSTA, M. da S.; ABREU, U. P.; BRANCO, J. da F. C.; MACHADO, T. M. M.; PIRES, L. C.; CAMPELO, J. E. G. |
Afiliação: |
Adriana Mello de Araújo, Embrapa Meio-Norte; Márcio da Silva Costa, UFPI; Urbano Pinto Abreu; José da Fonseca Castelo Branco, UFPI; Thea M. Medeiros Machado, UFV; Luanna Chácara Pires, UFV; José Elivalto Guimarães Campelo, UFPI. |
Título: |
Perfil sócio-econômico dos criatórios de caprinos tradicionais no Estado do Piauí e composição genética dos rebanhos. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
In: SIMPÓSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE, SIRGEALC, 7, 2009, Pucón, Chile. Proceeding... Santiago de Chile: Ministério de Agricultura, Instituto de Investigaciones Agropecuarias, 2009. |
Páginas: |
p. 225-226 |
Idioma: |
Português |
Thesagro: |
Caprino. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00795naa a2200205 a 4500 001 1658821 005 2010-02-25 008 2009 bl uuuu u00u1 u #d 100 1 $aARAÚJO, A. M. de 245 $aPerfil sócio-econômico dos criatórios de caprinos tradicionais no Estado do Piauí e composição genética dos rebanhos. 260 $c2009 300 $ap. 225-226 650 $aCaprino 700 1 $aCOSTA, M. da S. 700 1 $aABREU, U. P. 700 1 $aBRANCO, J. da F. C. 700 1 $aMACHADO, T. M. M. 700 1 $aPIRES, L. C. 700 1 $aCAMPELO, J. E. G. 773 $tIn: SIMPÓSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE, SIRGEALC, 7, 2009, Pucón, Chile. Proceeding... Santiago de Chile: Ministério de Agricultura, Instituto de Investigaciones Agropecuarias, 2009.
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