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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
20/10/1995 |
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Data da última atualização: |
20/10/1995 |
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Autoria: |
LURO, F.; LAIGRET, F.; BOVE, J. M.; OLLITRAULT, P. |
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Afiliação: |
Station de Recherche Agronomique de Corse, Institut National de la Recherche Agonomique, et al. Laboratoire de Biologie Celluaire et Moleculaire. |
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Título: |
DNA amplified fingerprinting, a useful tool for determination of genetic Origin and diversity analysis in citrus. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
HortScience, v.30, n.5, p.1063-1067, 1995. |
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Idioma: |
Inglês |
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Conteúdo: |
We used three short repetitive nucleotide sequences [(GTG)5, (TCC)5, and (GACA)4] either as radiolabeled probes for hybridization with restricted with restricted citrus DNA or as single primers in polymerase chain reaction amplication experiments with total genomic DNA. We tested the ability of the sequences to discriminate between seedlings of zygotic or nucellar origin in the progeny of a Volkamer lemon (Citrus volkameriana Ten. & Pasq.) tree. The genetic variability within two species [Citrus sinensis (L) Osbeck (sweet oranges) and Citrus reticulata Blanco and relatives (mandarins)] also was evaluated. DNA amplified fingerprinting with single primers was the more successful technique fordiscriminating between nucellar and zygotic seedlings. Although we were not able to distinguish among 10 cultivars of C. sinensis, all 10 reticulata cultivars tested were distinguishable. However, it still is difficult to indentiy the putative parents of a hybrid plant when the two parental genomes are closely related. |
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Palavras-Chave: |
PCR; RAPD. |
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Thesaurus Nal: |
plant breeding; polymorphism. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01582naa a2200205 a 4500
001 1634064
005 1995-10-20
008 1995 bl uuuu u00u1 u #d
100 1 $aLURO, F.
245 $aDNA amplified fingerprinting, a useful tool for determination of genetic Origin and diversity analysis in citrus.
260 $c1995
520 $aWe used three short repetitive nucleotide sequences [(GTG)5, (TCC)5, and (GACA)4] either as radiolabeled probes for hybridization with restricted with restricted citrus DNA or as single primers in polymerase chain reaction amplication experiments with total genomic DNA. We tested the ability of the sequences to discriminate between seedlings of zygotic or nucellar origin in the progeny of a Volkamer lemon (Citrus volkameriana Ten. & Pasq.) tree. The genetic variability within two species [Citrus sinensis (L) Osbeck (sweet oranges) and Citrus reticulata Blanco and relatives (mandarins)] also was evaluated. DNA amplified fingerprinting with single primers was the more successful technique fordiscriminating between nucellar and zygotic seedlings. Although we were not able to distinguish among 10 cultivars of C. sinensis, all 10 reticulata cultivars tested were distinguishable. However, it still is difficult to indentiy the putative parents of a hybrid plant when the two parental genomes are closely related.
650 $aplant breeding
650 $apolymorphism
653 $aPCR
653 $aRAPD
700 1 $aLAIGRET, F.
700 1 $aBOVE, J. M.
700 1 $aOLLITRAULT, P.
773 $tHortScience$gv.30, n.5, p.1063-1067, 1995.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Origem |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
30/01/2004 |
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Data da última atualização: |
25/01/2016 |
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Autoria: |
KESSLER, R. H.; GOMES, A.; CAPIBARIBE, P. R.; SILVA, D. da; SCHENK, M. A. M. |
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Afiliação: |
Embrapa Gado de Corte. (Campo Grande, MS). |
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Título: |
Estabelecimento de cultura in vitro de células embrionárias do carrapato Boophilus microplus. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Campo Grande: Embrapa Gado de Corte, 1999. |
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Páginas: |
4 p. |
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Série: |
(Embrapa Gado de Corte. Pesquisa em Andamento, 54). |
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Idioma: |
Português |
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Notas: |
CNPGC. |
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Palavras-Chave: |
In vitro. |
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Thesagro: |
Boophilus Microplus; Carrapato; Cultura de Célula. |
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Thesaurus NAL: |
ticks; tissue culture. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/137709/1/PESQ-EM-ANDAMENTO-54.pdf
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Marc: |
LEADER 00732nam a2200253 a 4500
001 1325492
005 2016-01-25
008 1999 bl uuuu u0uu1 u #d
100 1 $aKESSLER, R. H.
245 $aEstabelecimento de cultura in vitro de células embrionárias do carrapato Boophilus microplus.
260 $aCampo Grande: Embrapa Gado de Corte$c1999
300 $a4 p.
490 $a(Embrapa Gado de Corte. Pesquisa em Andamento, 54).
500 $aCNPGC.
650 $aticks
650 $atissue culture
650 $aBoophilus Microplus
650 $aCarrapato
650 $aCultura de Célula
653 $aIn vitro
700 1 $aGOMES, A.
700 1 $aCAPIBARIBE, P. R.
700 1 $aSILVA, D. da
700 1 $aSCHENK, M. A. M.
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Embrapa Gado de Corte (CNPGC) |
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Classificação |
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