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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
17/11/2015 |
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Data da última atualização: |
31/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROCHA, C. M. L.; VELLICCE, G. R.; GARCÍA, M. G.; PARDO, E. M.; RACEDO, J.; PERERA, M. F.; LUCÍA, A. de; GILLI, J.; BOGADO, N.; BONNECARRÈRE, V.; GERMAN, S.; MARCELINO, F.; LEDESMA, F.; REZNIKOV, S.; PLOPER, L. D.; WELIN, B.; CASTAGNARO, A. P. |
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Afiliação: |
CARLA MARIA LOURDES ROCHA, ITA - NOA; GABRIEL RICARDO VELLICCE, ITA - NOA; MARÍA GABRIELA GARCÍA, ITA - NOA; ESTEBAN MARIANO PARDO, ITA - NOA; JOSEFINA RACEDO, ITA - NOA; MARÍA FRANCISCA PERERA, ITA - NOA; ADRIAN DE LUCÍA, INTA; JAVIER GILLI, INTA; NOELIA BOGADO, IPTA; VICTORIA BONNECARRÈRE, INIA; SILVIA GERMAN, INIA; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; FERNANDO LEDESMA A ,, ITA - NOA; SEBASTIÁN REZNIKOV, ITA - NOA; LEONARDO DANIEL PLOPER, ITA - NOA; BJORN WELIN, ITA - NOA; ATILIO PEDRO CASTAGNARO, ITA- NOA. |
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Título: |
Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Electronic Journal of Biotechnology, v. 18, n. 6, p. 439-444, 2015. |
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ISSN: |
0717-3458 |
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DOI: |
10.1016/j.ejbt.2015.06.007 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies. MenosBackground: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and pot... Mostrar Tudo |
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Palavras-Chave: |
Ferrugem asiática da soja. |
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Thesagro: |
Doença de planta; Ferrugem; Fungo; Phakopsora pachyrhizi; Soja. |
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Thesaurus Nal: |
Microbial growth; Plant diseases and disorders; Soybean rust. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02845naa a2200445 a 4500
001 2028670
005 2017-07-31
008 2015 bl uuuu u00u1 u #d
022 $a0717-3458
024 7 $a10.1016/j.ejbt.2015.06.007$2DOI
100 1 $aROCHA, C. M. L.
245 $aUse of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi.$h[electronic resource]
260 $c2015
520 $aBackground: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies.
650 $aMicrobial growth
650 $aPlant diseases and disorders
650 $aSoybean rust
650 $aDoença de planta
650 $aFerrugem
650 $aFungo
650 $aPhakopsora pachyrhizi
650 $aSoja
653 $aFerrugem asiática da soja
700 1 $aVELLICCE, G. R.
700 1 $aGARCÍA, M. G.
700 1 $aPARDO, E. M.
700 1 $aRACEDO, J.
700 1 $aPERERA, M. F.
700 1 $aLUCÍA, A. de
700 1 $aGILLI, J.
700 1 $aBOGADO, N.
700 1 $aBONNECARRÈRE, V.
700 1 $aGERMAN, S.
700 1 $aMARCELINO, F.
700 1 $aLEDESMA, F.
700 1 $aREZNIKOV, S.
700 1 $aPLOPER, L. D.
700 1 $aWELIN, B.
700 1 $aCASTAGNARO, A. P.
773 $tElectronic Journal of Biotechnology$gv. 18, n. 6, p. 439-444, 2015.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 12 | |
| 3. |  | AMARAL, A. L. do; MORES, N.; ZANELLA, J. R. C.; COLDEBELLA, A.; RANGEL, L. F. S. Fornecimento de plasma suíno spray-dried diluído em água acidificada para leitões com sintomas de circovirose no início da fase de crescimento. Ciência Animal Brasileira, Goiânia, v. 12, n. 4, p. 681?686, 2011. Projeto/Plano de Ação: 03.06.00.89.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 6. | | LIMA, G. J. M. M. de; RANGEL, L. F. S.; MORAES, E.; FALCO, A. L.; CRENSHAW, J. D. Spray dried plasma in gestation and lactation diets improves piglet weight. In: CONGRESO COLÉGIO LATINOAMERICANO DE NUTRICIÓN ANIMAL, 5.; EXPOSIÇÃO LATINOAMERICANA INDUSTRIAL Y TECNOLÓGICA EM NUTRIÇÃO ANIMAL, 1., 2012, Puerto Vallarta, México. Memórias. Puerto Vallarta: CBNA, 2012. 1 CD-ROM. V CLANA. Projeto/Plano de Ação: 11.11.11.111.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 7. | | RANGEL, L. F. S.; MORÉS, N.; LIMA, G. J. M. M.; ZANCANARO, M.; LIMA, E. S. de; BARANCELII, G. V.; MIYADA, V. S.; MENTEN, J. F. M. Efeito do plasma na dieta de leitões desafiados pela circovirose. In: SIMPÓSIO SOBRE MANEJO E NUTRIÇÃO DE AVES E SUÍNOS, 5., 2008, Cascavel, PR. Anais. Campinas, SP: CBNA, 2008. p. 161-170. Projeto n.10.03.21.014-02.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 8. | | RANGEL, L. F. S.; ZANI, A.; CAPUTI, B.; DALMEDICO, G.; MILTENBURG, G.; BARANCELLI, G. V.; MIELE, M.; LIMA, G. J. M. M. de. Destinação de animais mortos. O que pensa a indústria de alimentação animal? Cadáveres devem ou não entrar na cadeia de alimentação animal? In: WORKSHOP CBNA SOBRE ALIMENTOS SEGUROS, 2017, Campinas. Anais... Campinas: CBNA, 2017.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 9. | | RANGEL, L. F. S.; CAMPBELL, J. M.; CRENSHAW, J. D.; BARANCELLI, G. V.; LIMA, G. J. M. M. de; MORÉS, N.; MIYADA, V. S.; MENTEN, J. F. M. Plasma ultrafiltrado spray dried. Suinocultura Industrial, v.30, n.216, p.24-32, 2008. Projeto/Plano de Ação: 16.00.30004-00.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 10. | | MORES, N.; ZANELLA, J. R. C.; AMARAL, A. L. do; COLDEBELLA, A.; LIMA, G. J. M. M. de; ZANELLA, E.; LIMA, E. S. de; RANGEL, L. F. S.; ZANCANARO, M.; GAVA, D. Prevenção da circovirose suína pelo uso do plasma suíno ultrafiltrado pelo processo "spray dried". Concórdia: Embrapa Suínos e Aves, 2007. 6 p. (Embrapa Suínos e Aves. Comunicado Técnico, 456) Projeto n. 10.06.21.032-01.| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 11. | | MORÉS, N.; RANGEL, L. F. S.; AMARAL, A. L. do; ZANELLA, J. R. C.; ZANCANARO, M.; LIMA, G. J. M. M. de; COLDEBELLA, A.; LIMA, E. S. de; MIELE, M. Uso do plasma sangüíneo produzido em sistema de spray dry (plasma) na prevenção da circovirose suína. Acta Scientiae Veterinariae, v.35, p.S209-S219, 2007. Suplemento. Projeto n. 10.06.11.010-01| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - C |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 12. | | MORÉS, N.; ZANELLA, J. R. C.; AMARAL, A. L. do; COLDEBELLA, A.; LIMA, G. J. M. M. de; MIELE, M.; ZANELLA, E. L.; RANGEL, L. F. S.; LIMA, E. S.; ZANCANARO, M. Spray dried porcine plasma in nursery and grower feed reduces the severity of porcine circovirus associated diseases. In: ALLEN D. LEMANN SWINE CONFERENCE, 2007, Minnesota. Proceedings... Miinnesota: College of Veterinary Medicine, University of Minnesota, 2007. v. 34. 1 CD-ROM. Projeto n. 10.06.21.032-01.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 12 | |
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