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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
03/09/1996 |
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Data da última atualização: |
03/09/1996 |
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Autoria: |
CARVALHO, L. J. C. B.; CASCARDO, J. C. M.; FERREIRA, M. A.; LOUREIRO, M. E. |
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Título: |
Studies on proteins and enzymes related to tuberization and starch biosynthesis in cassava roots. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
In: INTERNATIONAL SCIENTIFIC MEETING CASSAVA BIOTECHNOLOGY NETWORK, 1., 1992, Cartagena de Indias, Colombia. Proceedings. Cali: CIAT, 1993. |
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Páginas: |
p.234-238. |
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Idioma: |
Inglês |
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Conteúdo: |
A method has been developed to isolate intact cassava root amyloplast through protoplast preparation. Protoplasts were obtained after incubating cassava root slices in a medium containing cellulase and pectolyase for more than 10 hours at room temperature. After purification iln a discontinuous ficoll density agradient, the protoplasts were ruptured by forcing the suspension through modified disposable syringe. Amyloplast ilntactness was observed in a fluorescence microscopy and activity of amyloplast marker enzymes before and after rupture of the amyloplast membrane. Starch branching enzyme, ADPG-pyrophosphorylase, and starch symthetase were used as amyloplast marker enzymes. The results of the experiment are discussed in a broad starch biosynthesis and tube-rization project that is undergoing in CENARGEN. |
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Palavras-Chave: |
Byosynthesis. |
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Thesagro: |
Amido; Biossíntese; Enzima; Mandioca; Manihot Esculenta; Proteína. |
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Thesaurus Nal: |
cassava; enzymes; proteins; starch. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01696naa a2200301 a 4500
001 1556414
005 1996-09-03
008 1993 bl uuuu u00u1 u #d
100 1 $aCARVALHO, L. J. C. B.
245 $aStudies on proteins and enzymes related to tuberization and starch biosynthesis in cassava roots.
260 $c1993
300 $ap.234-238.
520 $aA method has been developed to isolate intact cassava root amyloplast through protoplast preparation. Protoplasts were obtained after incubating cassava root slices in a medium containing cellulase and pectolyase for more than 10 hours at room temperature. After purification iln a discontinuous ficoll density agradient, the protoplasts were ruptured by forcing the suspension through modified disposable syringe. Amyloplast ilntactness was observed in a fluorescence microscopy and activity of amyloplast marker enzymes before and after rupture of the amyloplast membrane. Starch branching enzyme, ADPG-pyrophosphorylase, and starch symthetase were used as amyloplast marker enzymes. The results of the experiment are discussed in a broad starch biosynthesis and tube-rization project that is undergoing in CENARGEN.
650 $acassava
650 $aenzymes
650 $aproteins
650 $astarch
650 $aAmido
650 $aBiossíntese
650 $aEnzima
650 $aMandioca
650 $aManihot Esculenta
650 $aProteína
653 $aByosynthesis
700 1 $aCASCARDO, J. C. M.
700 1 $aFERREIRA, M. A.
700 1 $aLOUREIRO, M. E.
773 $tIn: INTERNATIONAL SCIENTIFIC MEETING CASSAVA BIOTECHNOLOGY NETWORK, 1., 1992, Cartagena de Indias, Colombia. Proceedings. Cali: CIAT, 1993.
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Registro original: |
Embrapa Cerrados (CPAC) |
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