Data/hora: 20/06/2024 13:47:05

Biblioteca(s): 	Embrapa Café; Embrapa Unidades Centrais.	
Data corrente: 	01/03/2011	
Data da última atualização: 	04/03/2011	
Autoria: 	DIOLA, V.; BRITO, G. G. G.; GALLINA, A. P.; CAIXETA, E. T.; SAKYAMA, N. S.; LOUREIRO, M. E.	
Afiliação: 	UFV; UFV; BIOAGRO/UFV; EVELINE TEIXEIRA CAIXETA, SAPC; BIOAGRO/UFV; UFV/BIOAGRO.	
Título: 	High density genetic mapping of resistance gene to race II of Hemileia vstatrix in hibrido de timor genotype.	
Ano de publicação: 	2008	
Fonte/Imprenta: 	In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas.	
Idioma: 	Inglês	
Conteúdo: 	Coffee leaf rust caused by the fungus Hemileia vastatrix is the most important disease of Coffea Arabica, and can be responsible for reductions about 35 to 50% in coffee production in infected fields. In the Híbrido de Timor (HT) populations were detected resistance genes, that alone or in association, could confer resistance to several different physiologic races of H. vastatrix. The objective of this study was to generate a high density genetic map surrounding a single rust resistance gene. The mapping population (F2 UFV 421-4) was composed by 224 plants, derived from F1 self-fertilized individuals, which were derived from the cross between HT UFV 427-15 and the susceptible clone Catuai Amarelo IAC 30 (UFV 2143-236). Phenotypic characterization was performed by infection rate analysis of leaf disc inoculated with monospore cell culture rust race IIb spores. Phenotipic segregation in this population follow a rate of 3 resistant to 1 susceptible individual. Two BSAs (Bulked Segregant Analysis), one for resistant and other for susceptible plants, were composed by 5 genotypes each. About 770 primer combinations were used in AFLP analysis, and 15 markers were obtained. This marker delimited a chromosomal region of 90 cM. The nearest marker was positioned at 3.2 cM of the resistance gene, and it is inside a region of 6.6 cM between the markers limiting both gene sides. This make possible the obtaining of the physical map and to facility the studies of the signaling the defense answer in the interaction coffee x H. vastarix.	
Thesagro: 	Coffea Arábica; Hemileia Vastatrix.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Café (CNPCa)	
Exemplares: 	AI-SEDE - (49107-1) - EMB - AA - DD -  -  -  - DIS
CNPCa - (10-1) - UPC - AA - DD -  -  -  - DIS