Data/hora: 11/06/2024 10:06:17

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	25/04/1994	
Data da última atualização: 	25/04/1994	
Autoria: 	CARVALHO, L. J. C. B.; CASCARDO, J. C. M.; FERREIRA, M. A.; LOUREIRO, M. E.	
Afiliação: 	CENARGEN-EMBRAPA, Brasilia-DF, Brasil.	
Título: 	Studies on proteins and enzymes related to tuverization and starch biosynthesis in cassava roots.	
Ano de publicação: 	1993	
Fonte/Imprenta: 	Cali, Colombia: CIAT, 1993	
Páginas: 	234-238p	
Série: 	CIAT, Working Document,123	
Idioma: 	Inglês	
Notas: 	International scientific meeting Cassava Biotechnology Network, 1, 1992, Cartajena, Colombia. Proceedinjs... Cali, Colombia: CIAT, 1993.	
Conteúdo: 	A method has been developed to isolate intact cassava root amyloplast through protoplast proparation. Protoplasts were obtained after incubating cassava root slices in a medium containing cellulase and pectolyase for more than 10 hours at room temperature. After purification iln a discontinuous Ficoll density fradient, the protoplasts were ruptured by forcing the suspension through modified disposable syringe. Amyloplast ilntactness was observed in a fluorescence microscopy and activity of amyloplast marker enzymes before and after rupture of the amyloplast membrane. Starch branching enzyme, ADPG-pyrophosphorylase, and starch synthetase were used as amyloplast marker enzymes. The results of the experiment are discussed in a broad starch biosynthesis and tuber ization project that is undergoinf in CENARGEN.	
Palavras-Chave: 	Congresso.	
Thesagro: 	Biossíntese; Eletroforese; Meristema.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (13438-1) - ADD - PL - -- - 633.6820601 - I61p - 1993000070 - DIS