Data/hora: 23/09/2024 05:07:48

Biblioteca(s): 	Embrapa Suínos e Aves.	
Data corrente: 	03/11/2020	
Data da última atualização: 	03/11/2020	
Autoria: 	ROMANO, G. de S.; IBELLI, A. M. G.; LORENZETTI, W. R.; WEBER, T.; PEIXOTO, J. de O.; CANTAO, M. E.; MORES, M. A. Z.; MORÉS, N.; PEDROSA, V. B.; COUTINHO. L. L.; LEDUR, M. C.	
Afiliação: 	GABRIELI DE SOUZA ROMANO, UFBA; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; WILLIAN RAPHAEL LORENZETTI, UNICENTRO; TOMÁS WEBER, BRF; JANE DE OLIVEIRA PEIXOTO, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; MARCOS ANTONIO ZANELLA MORES, CNPSA; NELSON MORÉS, CNPSA; VICTOR BRENO PEDROSA, UEPG; LUIZ LEHMANN COUTINHO, ESALQ; MONICA CORREA LEDUR, CNPSA.	
Título: 	Inguinal ring RNA sequencing reveals downregulation of muscular genes related to scrotal hernia in pigs.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	Genes, v. 11, n. 117, 2020.	
DOI: 	https://doi.org/10.3390/genes11020117	
Idioma: 	Inglês	
Conteúdo: 	Abstract: Scrotal hernias (SH) are common congenital defects in commercial pigs, characterized by the presence of abdominal contents in the scrotal sac, leading to considerable production and animal welfare losses. Since the etiology of SH remains obscure, we aimed to identify the biological and genetic mechanisms involved in its occurrence through the whole transcriptome analysis of SH affected and unaffected pigs? inguinal rings. From the 22,452 genes annotated in the pig reference genome, 13,498 were expressed in the inguinal canal tissue. Of those, 703 genes were differentially expressed (DE, FDR < 0.05) between the two groups analyzed being, respectively, 209 genes upregulated and 494 downregulated in the SH-affected group. Thirty-seven significantly overrepresented GO terms related to SH were enriched, and the most relevant biological processes were muscular system, cell differentiation, sarcome reorganization, and myofibril assembly. The calcium signaling, hypertrophic cardiomyopathy, dilated cardiomyopathy, and cardiac muscle contraction were the major pathways possibly involved in the occurrence of the scrotal hernias. The expression profile of the DE genes was associated with the reduction of smooth muscle dierentiation, followed by low calcium content in the cell, which could lead to a decreased apoptosis ratio and diminished muscle contraction of the inguinal canal region. We have demonstrated that genes involved with musculature are closely linked to the physiological imbalance predisposing to scrotal hernia. According to our study, the genes MYBPC1, BOK, SLC25A4, SLC8A3, DES, TPM2, MAP1CL3C, and FGF1 were considered strong candidates for future evaluation.	
Palavras-Chave: 	Congenital disease; Differentially expressed genes; Doença congênita; Genes expressos diferencialmente; Hérnia escrotal; Sequenciamento do RNA; Transcriptoma.	
Thesagro: 	Genética Animal; Porco; Suíno.	
Thesaurus Nal: 	Animal genetics; Swine; Swine diseases; Transcriptome.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Suínos e Aves (CNPSA)	
Exemplares: 	CNPSA - (21953-1) - UPC - AP - DD -  -  -  - DIS