Data/hora: 16/06/2024 17:50:28

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 11/08/2021
Data da última atualização: | 11/08/2021
Autoria: | LOPES, J. M. L.; MATOS, E. M. de; ZORZATTO, C.; AZEVEDO, A. L. S.; MACHADO, M. A.; CHESTER, M.; VICCINI, L. F.
Afiliação: | JULIANA MAINENTI LEAL LOPES, Universidade Federal de Juiz de Fora; ELYABE MONTEIRO DE MATOS, Universidade Federal de Juiz de Fora; CRISTIANE ZORZATTO, Universidade Federal de Juiz de Fora; ANA LUISA SOUSA AZEVEDO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; MICHAEL CHESTER, Royal Botanic Gardens; LYDERSON FACIO VICCINI, Universidade Federal de Juiz de Fora.
Título: | Development of microsatellite markers for Lippia alba and related Lippia species.
Ano de publicação: | 2020
Fonte/Imprenta: | Molecular Biology Reports, v. 47, p. 4911-4915, 2020
DOI: | https://doi.org/10.1007/s11033-020-05445-z
Idioma: | Inglês
Conteúdo: | Microsatellite primers were developed in Lippia alba complex to better understanding the origins and evolution of the species. We sought to increase the numbers of available simple sequence repeat (SSR) markers. We performed low-coverage (~ twofold) genomic DNA sequencing of a diploid accession and generated a de novo assembly comprising 175,572 contigs. Sixteen SSR loci were selected and of these 13 SSR loci were successfully amplified in 20 L. alba tetraploid accessions and in 12 other Lippia species. Only one SSR locus was monomorphic, whereas 12 loci were polymorphic, yielding one to nine alleles. The heterozygosity was similar among markers, with values of 0.274?0.485; the polymorphism information content values varied from 0.237 to 0.367. These markers were successfully amplified in related species with 85% of transferability on average. Thus, we demonstrate the utility of including a de novo assembly step to obtain SSR markers from low-coverage genomic datasets.
Palavras-Chave: | Simple sequence repeat; SSR.
Thesagro: | Genética; Marcador Genético.
Thesaurus Nal: | Phyla (Verbenaceae); Polyploidy.
Categoria do assunto: | G Melhoramento Genético
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (25182-1) - UPC - AP - DD -  -  -  - DIS