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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
20/12/2010 |
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Data da última atualização: |
19/01/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
LOCALI-FABRIS, E. C.; MAFRA, V. S.; MARTINS, P. K.; RIBEIRO- ALVES, M.; FRANCISCO, C. S.; FREITAS ASTUA, J. de; KISHI, L. T.; MACHADO, M. A. |
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Afiliação: |
Eliane Cristina Locali-Fabris, APTA; Valéria S. Mafra, APTA; Polyana Kelly. Martins, APTA; Marcelo Ribeiro-Alves, Fiocruz; Carolina S. Francisco, APTA; JULIANA DE FREITAS ASTUA, CNPMF; Luciano T. Kishi, APTA; Marcelo A. Machado, APTA. |
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Título: |
Gene set enrichment analysis in sweet orange plants infected by Candidatus Liberibacter americanus. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. |
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Idioma: |
Inglês |
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Notas: |
0058 PSO
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010 |
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Conteúdo: |
Huanglongbing (HLB) is known as the most devastating disease of citrus. Two species of Candidatus Liberibacter have been reported in Brazil, Ca. L. asiaticus (CaLas) and Ca. L. americanus (CaLam). Since CaLas is prevalent worldwide, most of the studies have focused that species and very little information is available on CaLam. With the objective to investigate differences in metabolic pathways of Citrus sinensis in response to CaLam, we used a genome- wide microarray approach to identify differentially expressed genes in symptomatic plants when compared to control plants. Hybridizations were performed by Roche Nimblegen with a customized 385K chip containing 32,000 unigenes of C. sinensis. The raw data were pre-processed using the Robust Multiarray Average (RMA). Normalized data were imported to R/Bioconductor where statistical analyses were performed. A Bayesian moderated t-test was calculated and p-values were adjusted for multiple comparisons by the false discovery rate correction. A total of 634 genes with P-values ?0.05, fold change |FC| ? 2.0 and odds probability ? 0.95 were considered differentially expressed. From those, 419 were up- and 215 down-regulated in symptomatic plants. In order to identify enriched gene ontologies regulated in infected plants we applied a gene set enrichment analysis approach and found 45 different biological processes including those involved in response to jasmonic acid stimulus, signaling and metabolism, 17 molecular functions, including oxygen binding, monooxygenase activity and oxidoreductase activity, 6 cellular components, mostly related to chloroplast, enriched at a statistically significant level of 0.001. MenosHuanglongbing (HLB) is known as the most devastating disease of citrus. Two species of Candidatus Liberibacter have been reported in Brazil, Ca. L. asiaticus (CaLas) and Ca. L. americanus (CaLam). Since CaLas is prevalent worldwide, most of the studies have focused that species and very little information is available on CaLam. With the objective to investigate differences in metabolic pathways of Citrus sinensis in response to CaLam, we used a genome- wide microarray approach to identify differentially expressed genes in symptomatic plants when compared to control plants. Hybridizations were performed by Roche Nimblegen with a customized 385K chip containing 32,000 unigenes of C. sinensis. The raw data were pre-processed using the Robust Multiarray Average (RMA). Normalized data were imported to R/Bioconductor where statistical analyses were performed. A Bayesian moderated t-test was calculated and p-values were adjusted for multiple comparisons by the false discovery rate correction. A total of 634 genes with P-values ?0.05, fold change |FC| ? 2.0 and odds probability ? 0.95 were considered differentially expressed. From those, 419 were up- and 215 down-regulated in symptomatic plants. In order to identify enriched gene ontologies regulated in infected plants we applied a gene set enrichment analysis approach and found 45 different biological processes including those involved in response to jasmonic acid stimulus, signaling and metabolism, 17 molecular functions, includin... Mostrar Tudo |
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Palavras-Chave: |
Huanglongbing; Plant disease. |
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Thesagro: |
Doença de Planta; Fruta Cítrica. |
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Thesaurus Nal: |
Candidatus Liberibacter; Citrus. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02656nam a2200277 a 4500
001 1870461
005 2011-01-19
008 2010 bl uuuu u00u1 u #d
100 1 $aLOCALI-FABRIS, E. C.
245 $aGene set enrichment analysis in sweet orange plants infected by Candidatus Liberibacter americanus.
260 $aIn: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM.$c2010
500 $a0058 PSO Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010
520 $aHuanglongbing (HLB) is known as the most devastating disease of citrus. Two species of Candidatus Liberibacter have been reported in Brazil, Ca. L. asiaticus (CaLas) and Ca. L. americanus (CaLam). Since CaLas is prevalent worldwide, most of the studies have focused that species and very little information is available on CaLam. With the objective to investigate differences in metabolic pathways of Citrus sinensis in response to CaLam, we used a genome- wide microarray approach to identify differentially expressed genes in symptomatic plants when compared to control plants. Hybridizations were performed by Roche Nimblegen with a customized 385K chip containing 32,000 unigenes of C. sinensis. The raw data were pre-processed using the Robust Multiarray Average (RMA). Normalized data were imported to R/Bioconductor where statistical analyses were performed. A Bayesian moderated t-test was calculated and p-values were adjusted for multiple comparisons by the false discovery rate correction. A total of 634 genes with P-values ?0.05, fold change |FC| ? 2.0 and odds probability ? 0.95 were considered differentially expressed. From those, 419 were up- and 215 down-regulated in symptomatic plants. In order to identify enriched gene ontologies regulated in infected plants we applied a gene set enrichment analysis approach and found 45 different biological processes including those involved in response to jasmonic acid stimulus, signaling and metabolism, 17 molecular functions, including oxygen binding, monooxygenase activity and oxidoreductase activity, 6 cellular components, mostly related to chloroplast, enriched at a statistically significant level of 0.001.
650 $aCandidatus Liberibacter
650 $aCitrus
650 $aDoença de Planta
650 $aFruta Cítrica
653 $aHuanglongbing
653 $aPlant disease
700 1 $aMAFRA, V. S.
700 1 $aMARTINS, P. K.
700 1 $aRIBEIRO- ALVES, M.
700 1 $aFRANCISCO, C. S.
700 1 $aFREITAS ASTUA, J. de
700 1 $aKISHI, L. T.
700 1 $aMACHADO, M. A.
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Esconder MarcMostrar Marc Completo |
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
20/07/1995 |
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Data da última atualização: |
21/10/2021 |
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Autoria: |
SILVA, J. B. C. da; LOPES, C. A. (org.). |
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Afiliação: |
JOÃO BOSCO C. DA SILVA, EMBRAPA-CNPH; CARLOS ALBERTO LOPES, CNPH. |
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Título: |
Cultivo da batata-doce (Ipomoea batatas (L.) Lam). |
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Edição: |
3. ed. rev. e ampl. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Brasilia: EMBRAPA-CNPH, 1995. |
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Páginas: |
18 p. |
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Série: |
(EMBRAPA-CNPH. Instruções técnicas, 7). |
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ISSN: |
0102-6534 |
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Idioma: |
Português |
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Conteúdo: |
Exigências climáticas. Solos. Preparo do solo e calagem. Adubação. Cultivares. Época de plantio. Formas de propagação. Formação do viveiro. Espaçamento. Plantio. Tratos culturais. Irrigação. Doenças. Mal-do-pé. Ferrugem-branca. Sarna. Mancha-de-alternária. Mancha-parda. Podridão-mole. Micoplasmose. Medidas gerais de controle de doenças. Distúrbios fisiológicos. Pragas. Colheita. Classificação e embalagem. Coeficientes técnicos (para 1 hectare). |
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Palavras-Chave: |
Batata-doce; Brasil; Classificação; Cropping; Cultivation; Cultivo; Diseases; Pest insects; Production; Sweet potato. |
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Thesagro: |
Adubação; Batata Doce; Cerrado; Colheita; Doença; Doença de Planta; Embalagem; Hortaliça; Inseto; Ipomoea Batatas; Irrigação; Plantio; Praga; Praga de Planta; Produção; Solo; Variedade. |
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Thesaurus NAL: |
Brazil; classification; fertilizers; harvesting; irrigation; packaging; soil. |
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Categoria do assunto: |
--
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140444/1/CNPH-Instrucoes-tecnicas-7.pdf
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Marc: |
LEADER 01831nam a2200577 a 4500
001 1753382
005 2021-10-21
008 1995 bl uuuu u0uu1 u #d
022 $a0102-6534
100 1 $aSILVA, J. B. C. da
245 $aCultivo da batata-doce (Ipomoea batatas (L.) Lam).
250 $a3. ed. rev. e ampl.
260 $aBrasilia: EMBRAPA-CNPH$c1995
300 $a18 p.
490 $a(EMBRAPA-CNPH. Instruções técnicas, 7).
520 $aExigências climáticas. Solos. Preparo do solo e calagem. Adubação. Cultivares. Época de plantio. Formas de propagação. Formação do viveiro. Espaçamento. Plantio. Tratos culturais. Irrigação. Doenças. Mal-do-pé. Ferrugem-branca. Sarna. Mancha-de-alternária. Mancha-parda. Podridão-mole. Micoplasmose. Medidas gerais de controle de doenças. Distúrbios fisiológicos. Pragas. Colheita. Classificação e embalagem. Coeficientes técnicos (para 1 hectare).
650 $aBrazil
650 $aclassification
650 $afertilizers
650 $aharvesting
650 $airrigation
650 $apackaging
650 $asoil
650 $aAdubação
650 $aBatata Doce
650 $aCerrado
650 $aColheita
650 $aDoença
650 $aDoença de Planta
650 $aEmbalagem
650 $aHortaliça
650 $aInseto
650 $aIpomoea Batatas
650 $aIrrigação
650 $aPlantio
650 $aPraga
650 $aPraga de Planta
650 $aProdução
650 $aSolo
650 $aVariedade
653 $aBatata-doce
653 $aBrasil
653 $aClassificação
653 $aCropping
653 $aCultivation
653 $aCultivo
653 $aDiseases
653 $aPest insects
653 $aProduction
653 $aSweet potato
700 1 $aLOPES, C. A.
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Embrapa Hortaliças (CNPH) |
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