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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
29/03/2016 |
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Data da última atualização: |
28/03/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AGUIRRE, A. de A. R.; LOBO, F. P.; ANDREOTTI, R. |
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Afiliação: |
ANDRÉ DE ABREU RANGEL AGUIRRE, FAMEZ/UFMS; FRANCISCO PEREIRA LOBO, CNPTIA; RENATO ANDREOTTI E SILVA, CNPGC. |
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Título: |
Design of the ATAQ peptide and its evaluation as an immunogen to develop a Rhipicephalus vaccine. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 221, p. 30-38, 2016 |
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Idioma: |
Inglês |
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Conteúdo: |
Tick infestation may cause several problems including affecting domestic animal health and reducing the production of meat and milk, among others. Resistance to several classes of acaricides have been reported, forcing researchers to search for alternative measures, such as vaccines against ticks, to ensure tick control while having no or at least low negative impacts on the environment and public health. However, the current commercially available vaccines in different strains of Rhipicephalus microplus are reported to be of low efficacy. Fortunately, reverse vaccinology approaches have shown positive results in the new generation of vaccines. On this basis, a synthetic peptide from the ATAQ protein, which is present in the gut and Malpighi tubes of R. microplus, was synthesized. The ATAQ proteins were isolated, characterized and sequenced from several species of the genus Rhipicephalus. The alignment showed 93.3% identity among DNA sequences of ATAQs from these species. Because of this, immunization trials with this peptide were conducted on mice, rabbits and cattle to evaluate the humoral immune response and the efficacy against Rhipicephalus sanguineus in addition to R. microplus. Based on recent results, we conclude that reverse vaccinology is a promising approach because it is more accurate and faster than conventional methods in the detection of potential antigens to use in anti-tick vaccines. It is not only applicable against R. microplus but also against tick species that play important roles in spreading other diseases. ATAQ proteins should be considered as the antigen in new trials to develop a multi-antigenic vaccine. Although these peptides behave as hapten and are not able to be recognized by the immune system on its own, using carriers and adjuvants helps its presentation and induces strong immune responses. Furthermore, an efficiency of 35% reduction in overall life cycle parameters was reported for R. microplus (98% for ELISA responder animals) and 47% for R. sanguineus. Although not yet enough to prevent the environment to infestation of ticks, this still constitutes a promising strategy that could be applied to integrated measures on tick control and in new research that develops anti-tick vaccines. MenosTick infestation may cause several problems including affecting domestic animal health and reducing the production of meat and milk, among others. Resistance to several classes of acaricides have been reported, forcing researchers to search for alternative measures, such as vaccines against ticks, to ensure tick control while having no or at least low negative impacts on the environment and public health. However, the current commercially available vaccines in different strains of Rhipicephalus microplus are reported to be of low efficacy. Fortunately, reverse vaccinology approaches have shown positive results in the new generation of vaccines. On this basis, a synthetic peptide from the ATAQ protein, which is present in the gut and Malpighi tubes of R. microplus, was synthesized. The ATAQ proteins were isolated, characterized and sequenced from several species of the genus Rhipicephalus. The alignment showed 93.3% identity among DNA sequences of ATAQs from these species. Because of this, immunization trials with this peptide were conducted on mice, rabbits and cattle to evaluate the humoral immune response and the efficacy against Rhipicephalus sanguineus in addition to R. microplus. Based on recent results, we conclude that reverse vaccinology is a promising approach because it is more accurate and faster than conventional methods in the detection of potential antigens to use in anti-tick vaccines. It is not only applicable against R. microplus but also against tick specie... Mostrar Tudo |
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Palavras-Chave: |
ATAQ; Reverse vaccinology; Synthetic peptide. |
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Thesaurus Nal: |
Immunization; Rhipicephalus microplus; Rhipicephalus sanguineus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/158239/1/Design-of-the-ATAQ-peptide-and-its-evaluation.pdf
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Marc: |
LEADER 02914naa a2200217 a 4500
001 2041997
005 2017-03-28
008 2016 bl uuuu u00u1 u #d
100 1 $aAGUIRRE, A. de A. R.
245 $aDesign of the ATAQ peptide and its evaluation as an immunogen to develop a Rhipicephalus vaccine.$h[electronic resource]
260 $c2016
520 $aTick infestation may cause several problems including affecting domestic animal health and reducing the production of meat and milk, among others. Resistance to several classes of acaricides have been reported, forcing researchers to search for alternative measures, such as vaccines against ticks, to ensure tick control while having no or at least low negative impacts on the environment and public health. However, the current commercially available vaccines in different strains of Rhipicephalus microplus are reported to be of low efficacy. Fortunately, reverse vaccinology approaches have shown positive results in the new generation of vaccines. On this basis, a synthetic peptide from the ATAQ protein, which is present in the gut and Malpighi tubes of R. microplus, was synthesized. The ATAQ proteins were isolated, characterized and sequenced from several species of the genus Rhipicephalus. The alignment showed 93.3% identity among DNA sequences of ATAQs from these species. Because of this, immunization trials with this peptide were conducted on mice, rabbits and cattle to evaluate the humoral immune response and the efficacy against Rhipicephalus sanguineus in addition to R. microplus. Based on recent results, we conclude that reverse vaccinology is a promising approach because it is more accurate and faster than conventional methods in the detection of potential antigens to use in anti-tick vaccines. It is not only applicable against R. microplus but also against tick species that play important roles in spreading other diseases. ATAQ proteins should be considered as the antigen in new trials to develop a multi-antigenic vaccine. Although these peptides behave as hapten and are not able to be recognized by the immune system on its own, using carriers and adjuvants helps its presentation and induces strong immune responses. Furthermore, an efficiency of 35% reduction in overall life cycle parameters was reported for R. microplus (98% for ELISA responder animals) and 47% for R. sanguineus. Although not yet enough to prevent the environment to infestation of ticks, this still constitutes a promising strategy that could be applied to integrated measures on tick control and in new research that develops anti-tick vaccines.
650 $aImmunization
650 $aRhipicephalus microplus
650 $aRhipicephalus sanguineus
653 $aATAQ
653 $aReverse vaccinology
653 $aSynthetic peptide
700 1 $aLOBO, F. P.
700 1 $aANDREOTTI, R.
773 $tVeterinary Parasitology$gv. 221, p. 30-38, 2016
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas; Embrapa Unidades Centrais. |
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Data corrente: |
10/02/2021 |
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Data da última atualização: |
10/02/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
FRAGOSO, R. de O.; CARPANEZZI, A. A.; ZUFFELLATO-RIBAS, K. C.; KOEHLER, H. S. |
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Afiliação: |
ROSIMERI de OLIVEIRA FRAGOSO., Universidade Federal do Paraná, Departamento de Fitotecnia e Fitossanitarismo; ANTONIO APARECIDO CARPANEZZI, CNPF; KATIA CHRISTINA ZUFFELLATO-RIBAS, Universidade Federal do Paraná, Setor de Ciências Biológicas, Departamento de Botânica; HENRIQUE SOARES KOEHLER, Universidade Federal do Paraná, Departamento de Fitotecnia e Fitossanitarismo. |
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Título: |
Forest restoration by different nucleation techniques in Urochloa grassland. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, v.56, e02119, 2021. |
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DOI: |
https://doi.org/10.1590/S1678-3921. pab2021.v56.02119 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT - The objective of this work was to evaluate the effect of brushwood, black plastic mulch, herbicide, and artificial perch on the natural regeneration of native species in Urochloa grassland. The experiment was conducted between February 2014 and February 2016 in the Dense Ombrophilous Forest, in the municipality of Morretes, in the state of Paraná, Brazil. The treatments were: herbicide, herbicide + perch, black plastic mulch, black plastic mulch + perch, brushwood + herbicide, brushwood + herbicide + perch, and a control treatment. The evaluations were carried out at 4, 8, 12, 18, and 24 months after the installation of the experiment, by counting and identifying regenerating woody species and estimating visually the percentage of herbaceous coverage. Initially, brushwood and black plastic mulch reduced the Urochloa grasses; however, this effect was lost over time due to the rapid growth of the grasses from the edges to the center of the plots. The use of perches in the treatments does not allow a significant increase of other species because of the continued inhibiting conditions for the establishment of seedlings. The herbicide is effective in removing the grasses; however, the recruitment of woody species is only satisfactory when perches are used to attract the dispersing fauna. For a successful ecological restoration of pastures, there is a need for the local elimination of Urochloa grasses.
RESUMO - O objetivo deste trabalho foi avaliar os métodos galharia, cobertura por plástico preto, herbicida e poleiro artificial na regeneração natural de espécies nativas em área com pastagem de Urochloa. O experimento foi conduzido entre fevereiro de 2014 e fevereiro de 2016 na Floresta Ombrófila Densa, no município de Morretes, no estado do Paraná, Brasil. Os tratamentos foram: herbicida, herbicida + poleiro, cobertura por plástico preto, cobertura por plástico preto + poleiro, galharia + herbicida, galharia + herbicida + poleiro e testemunha. As avaliações foram realizadas aos 4, 8, 12, 18 e 24 meses após a instalação do experimento, com contagem e identificação das espécies lenhosas regenerantes e estimativa visual do percentual de cobertura por herbáceas. Inicialmente, a galharia e a cobertura por plástico preto reduziram as gramíneas Urochloa; entretanto, com o decorrer do tempo, esse efeito se perdeu devido ao rápido crescimento das gramíneas das bordas para o centro das parcelas. O uso de poleiros nos tratamentos não possibilita incremento significativo de outras espécies, em razão da permanência das condições inibidoras ao estabelecimento das plântulas. O herbicida é eficiente para remover as gramíneas; contudo, o recrutamento de espécies lenhosas somente é satisfatório com o uso de poleiros para atração da fauna dispersora. Para o sucesso da restauração ecológica em pastagens, há a necessidade de eliminação local das gramíneas Urochloa MenosABSTRACT - The objective of this work was to evaluate the effect of brushwood, black plastic mulch, herbicide, and artificial perch on the natural regeneration of native species in Urochloa grassland. The experiment was conducted between February 2014 and February 2016 in the Dense Ombrophilous Forest, in the municipality of Morretes, in the state of Paraná, Brazil. The treatments were: herbicide, herbicide + perch, black plastic mulch, black plastic mulch + perch, brushwood + herbicide, brushwood + herbicide + perch, and a control treatment. The evaluations were carried out at 4, 8, 12, 18, and 24 months after the installation of the experiment, by counting and identifying regenerating woody species and estimating visually the percentage of herbaceous coverage. Initially, brushwood and black plastic mulch reduced the Urochloa grasses; however, this effect was lost over time due to the rapid growth of the grasses from the edges to the center of the plots. The use of perches in the treatments does not allow a significant increase of other species because of the continued inhibiting conditions for the establishment of seedlings. The herbicide is effective in removing the grasses; however, the recruitment of woody species is only satisfactory when perches are used to attract the dispersing fauna. For a successful ecological restoration of pastures, there is a need for the local elimination of Urochloa grasses.
RESUMO - O objetivo deste trabalho foi avaliar os métodos galharia,... Mostrar Tudo |
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Thesagro: |
Espécie Nativa; Gramínea; Herbicida; Nucleação; Planta Lenhosa; Reflorestamento; Regeneração Natural. |
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Thesaurus NAL: |
Grasslands; Herbicides; Natural regeneration; Perch; Urochloa; Woody plants. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/221072/1/Forest-restoration-different-2021.pdf
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Marc: |
LEADER 03889naa a2200325 a 4500
001 2129923
005 2021-02-10
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S1678-3921. pab2021.v56.02119$2DOI
100 1 $aFRAGOSO, R. de O.
245 $aForest restoration by different nucleation techniques in Urochloa grassland.$h[electronic resource]
260 $c2021
520 $aABSTRACT - The objective of this work was to evaluate the effect of brushwood, black plastic mulch, herbicide, and artificial perch on the natural regeneration of native species in Urochloa grassland. The experiment was conducted between February 2014 and February 2016 in the Dense Ombrophilous Forest, in the municipality of Morretes, in the state of Paraná, Brazil. The treatments were: herbicide, herbicide + perch, black plastic mulch, black plastic mulch + perch, brushwood + herbicide, brushwood + herbicide + perch, and a control treatment. The evaluations were carried out at 4, 8, 12, 18, and 24 months after the installation of the experiment, by counting and identifying regenerating woody species and estimating visually the percentage of herbaceous coverage. Initially, brushwood and black plastic mulch reduced the Urochloa grasses; however, this effect was lost over time due to the rapid growth of the grasses from the edges to the center of the plots. The use of perches in the treatments does not allow a significant increase of other species because of the continued inhibiting conditions for the establishment of seedlings. The herbicide is effective in removing the grasses; however, the recruitment of woody species is only satisfactory when perches are used to attract the dispersing fauna. For a successful ecological restoration of pastures, there is a need for the local elimination of Urochloa grasses. RESUMO - O objetivo deste trabalho foi avaliar os métodos galharia, cobertura por plástico preto, herbicida e poleiro artificial na regeneração natural de espécies nativas em área com pastagem de Urochloa. O experimento foi conduzido entre fevereiro de 2014 e fevereiro de 2016 na Floresta Ombrófila Densa, no município de Morretes, no estado do Paraná, Brasil. Os tratamentos foram: herbicida, herbicida + poleiro, cobertura por plástico preto, cobertura por plástico preto + poleiro, galharia + herbicida, galharia + herbicida + poleiro e testemunha. As avaliações foram realizadas aos 4, 8, 12, 18 e 24 meses após a instalação do experimento, com contagem e identificação das espécies lenhosas regenerantes e estimativa visual do percentual de cobertura por herbáceas. Inicialmente, a galharia e a cobertura por plástico preto reduziram as gramíneas Urochloa; entretanto, com o decorrer do tempo, esse efeito se perdeu devido ao rápido crescimento das gramíneas das bordas para o centro das parcelas. O uso de poleiros nos tratamentos não possibilita incremento significativo de outras espécies, em razão da permanência das condições inibidoras ao estabelecimento das plântulas. O herbicida é eficiente para remover as gramíneas; contudo, o recrutamento de espécies lenhosas somente é satisfatório com o uso de poleiros para atração da fauna dispersora. Para o sucesso da restauração ecológica em pastagens, há a necessidade de eliminação local das gramíneas Urochloa
650 $aGrasslands
650 $aHerbicides
650 $aNatural regeneration
650 $aPerch
650 $aUrochloa
650 $aWoody plants
650 $aEspécie Nativa
650 $aGramínea
650 $aHerbicida
650 $aNucleação
650 $aPlanta Lenhosa
650 $aReflorestamento
650 $aRegeneração Natural
700 1 $aCARPANEZZI, A. A.
700 1 $aZUFFELLATO-RIBAS, K. C.
700 1 $aKOEHLER, H. S.
773 $tPesquisa Agropecuária Brasileira$gv.56, e02119, 2021.
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Embrapa Florestas (CNPF) |
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