Data/hora: 01/06/2024 00:54:04

Biblioteca(s): 	Embrapa Agricultura Digital; Embrapa Pesca e Aquicultura; Embrapa Recursos Genéticos e Biotecnologia.	
Data corrente: 	11/11/2020	
Data da última atualização: 	22/01/2021	
Autoria: 	CARRIERO, M. M.; HENRIQUE SILVA, F.; MEIRA, C. M.; GATO, I. M. Q.; CAETANO, A. R.; LOBO, F. P.; ALVES, A. L.; VARELA, E. S.; MAIA, A. A. M.	
Afiliação: 	MATEUS MALDONADO CARRIERO, USP; FLAVIO HENRIQUE SILVA, UFSCAR; CAROLINE MUNHOZ MEIRA, USP; IGOR MATEUS QUEIROZ GATO, USP; ALEXANDRE RODRIGUES CAETANO, Cenargen; FRANCISCO PEREIRA LOBO; ANDERSON LUIS ALVES, SIN; EDUARDO SOUSA VARELA, CNPASA; ANTONIO AUGUSTO MENDES MAIA, USP.	
Título: 	Molecular characterization and gene expression analysis of the pro-inflammatory cytokines IL-1beta and IL-8 in the South American fish Piaractus mesopotamicus challenged with Aeromonas dhakensis.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	Genetics and Molecular Biology, v. 43, n. 4, e20200006, 2020.	
ISSN: 	1678-4685	
DOI: 	https://doi.org/10.1590/1678-4685-gmb-2020-0006	
Idioma: 	Inglês	
Conteúdo: 	Abstract. In the present study, the complete characterization of cDNA and genomic sequences of IL-1 and IL-8, as well as the expression profile of these genes in the South American fish pacu (Piaractus mesopotamicus) is provided. The full-length pmIL-1 cDNA was composed of 1208 nucleotides that would produce a precursor peptide with 273 amino acid residues. A putative caspase-1 cleavage site, similar to what is found in mammalian IL-1, was identified producing a mature peptide with a theoretical molecular weight of 17.21 kDa. The pmIL-8 cDNA sequence consisted of 1019 nucleotides which encoded a 95-amino acid protein with a theoretical molecular weight of 10.43 kDa that showed all typical CXC chemokine features, including a 20-residue signal peptide and four conserved cysteine residues. Constitutive mRNA expression was detected for both genes in the liver, head kidney, gill, intestine, skin and spleen. After a bacterial challenge, up-regulation was detected for both pmIL-1 and pmIL-8 in the spleen and head kidney at 12 h post-infection. At 24 h post-infection there was a decrease in the expression of both genes, with pmIL-8 showing a significant down-regulation in the liver and head kidney when compared to the control groups	
Palavras-Chave: 	Bacterial infection; Expressão gênica; Fish immunology; Imunologia de peixe; Infecção bacteriana; Interleukin; South American fish.	
Thesagro: 	Infecção; Pacu; Peixe.	
Thesaurus Nal: 	Fish; Gene expression; Interleukins; Piaractus mesopotamicus.	
Categoria do assunto: 	--
L Ciência Animal e Produtos de Origem Animal	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/220516/1/gmb-2020.pdf	
Registro original: 	Embrapa Pesca e Aquicultura (CNPASA)	
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