| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
31/03/2005 |
|
Data da última atualização: |
31/03/2005 |
|
Autoria: |
LIU, S. M.; MURRAY, A.; SCHLINK, A. C.; MATA, G.; MASTERS, D. G. |
|
Título: |
The effects of intradermal injections of spermidine on the growth rate of fibres and mitosis of wool follicles in Merino lambs. |
|
Ano de publicação: |
2002 |
|
Fonte/Imprenta: |
Animal Science, v. 75, pt 1, p. 33-40, 2002. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Polyamines (putrescine, spermidine and spermine) are required for optimal growth in alI cells, and are essential for cell proliferation and growth of cultured wool follicles, with an optimal concentration of spermidine required for the fibre elongation. The effects of a local supply of exogenous spermidine on the rate of cell division in the wool follicles, the length growth rate and diameter of fibres were therefore examined in Merino lambs. Three groups of eight lambs (40 kg) were given food at 1.2 X maintenance. Spermidine was injected intradermally into a small patch (3 X 3 cm) on the left flank three times per day for 7 days at one of three concentrations: 1.38, 2.75 or 4.58 mmol in 0.8 ml volume. The same volume of saline was injected into the contralateral side as a control. The concentration of spermidine in the skin patch 3 h after injection on day 7 increased by proportionately 0-18,0.33 or 0-41 (P < 0.001) respectively. The rates of cell division in lhe follicle bulb 3 h after the spermidine injection were proportionately 0.104, 0.184 and 0.283 higher compared with the contralateral side (P = 0.078 overall) for lhe low, medium and high doses of spermidine respectively and differed between the three doses (P < 0.05). The fibre length growth rate, as measured using autoradiography, was proportionately 0-099, 0.117 and 0-156 higher than that of the contralateral side (P < 0.001 overall) for lhe low, medium and high doses of spermidine respectively, but differences between doses were not significant (P> 0.05). Spermidine injection did not result in a significant change in fibre diameter during the treatment period. The ratio of fibre length growth rate to fibre diameter was increased by the injection of spermidine (P < 0.001). The results suggest that injecting extra spermidine into lhe skin altered spermidine homeostasis in the skin, stimulated cell proliferation and resulted in increased fibre growth. MenosPolyamines (putrescine, spermidine and spermine) are required for optimal growth in alI cells, and are essential for cell proliferation and growth of cultured wool follicles, with an optimal concentration of spermidine required for the fibre elongation. The effects of a local supply of exogenous spermidine on the rate of cell division in the wool follicles, the length growth rate and diameter of fibres were therefore examined in Merino lambs. Three groups of eight lambs (40 kg) were given food at 1.2 X maintenance. Spermidine was injected intradermally into a small patch (3 X 3 cm) on the left flank three times per day for 7 days at one of three concentrations: 1.38, 2.75 or 4.58 mmol in 0.8 ml volume. The same volume of saline was injected into the contralateral side as a control. The concentration of spermidine in the skin patch 3 h after injection on day 7 increased by proportionately 0-18,0.33 or 0-41 (P < 0.001) respectively. The rates of cell division in lhe follicle bulb 3 h after the spermidine injection were proportionately 0.104, 0.184 and 0.283 higher compared with the contralateral side (P = 0.078 overall) for lhe low, medium and high doses of spermidine respectively and differed between the three doses (P < 0.05). The fibre length growth rate, as measured using autoradiography, was proportionately 0-099, 0.117 and 0-156 higher than that of the contralateral side (P < 0.001 overall) for lhe low, medium and high doses of spermidine respectively, but differences be... Mostrar Tudo |
|
Palavras-Chave: |
Espermidina; Poliamina. |
|
Thesagro: |
Cordeiro; Lã; Merino; Metionina; Ovino. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02629naa a2200253 a 4500
001 1531036
005 2005-03-31
008 2002 bl uuuu u00u1 u #d
100 1 $aLIU, S. M.
245 $aThe effects of intradermal injections of spermidine on the growth rate of fibres and mitosis of wool follicles in Merino lambs.
260 $c2002
520 $aPolyamines (putrescine, spermidine and spermine) are required for optimal growth in alI cells, and are essential for cell proliferation and growth of cultured wool follicles, with an optimal concentration of spermidine required for the fibre elongation. The effects of a local supply of exogenous spermidine on the rate of cell division in the wool follicles, the length growth rate and diameter of fibres were therefore examined in Merino lambs. Three groups of eight lambs (40 kg) were given food at 1.2 X maintenance. Spermidine was injected intradermally into a small patch (3 X 3 cm) on the left flank three times per day for 7 days at one of three concentrations: 1.38, 2.75 or 4.58 mmol in 0.8 ml volume. The same volume of saline was injected into the contralateral side as a control. The concentration of spermidine in the skin patch 3 h after injection on day 7 increased by proportionately 0-18,0.33 or 0-41 (P < 0.001) respectively. The rates of cell division in lhe follicle bulb 3 h after the spermidine injection were proportionately 0.104, 0.184 and 0.283 higher compared with the contralateral side (P = 0.078 overall) for lhe low, medium and high doses of spermidine respectively and differed between the three doses (P < 0.05). The fibre length growth rate, as measured using autoradiography, was proportionately 0-099, 0.117 and 0-156 higher than that of the contralateral side (P < 0.001 overall) for lhe low, medium and high doses of spermidine respectively, but differences between doses were not significant (P> 0.05). Spermidine injection did not result in a significant change in fibre diameter during the treatment period. The ratio of fibre length growth rate to fibre diameter was increased by the injection of spermidine (P < 0.001). The results suggest that injecting extra spermidine into lhe skin altered spermidine homeostasis in the skin, stimulated cell proliferation and resulted in increased fibre growth.
650 $aCordeiro
650 $aLã
650 $aMerino
650 $aMetionina
650 $aOvino
653 $aEspermidina
653 $aPoliamina
700 1 $aMURRAY, A.
700 1 $aSCHLINK, A. C.
700 1 $aMATA, G.
700 1 $aMASTERS, D. G.
773 $tAnimal Science$gv. 75, pt 1, p. 33-40, 2002.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
23/03/2006 |
|
Data da última atualização: |
22/02/2016 |
|
Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
|
Autoria: |
LEAL-BERTIOLI, S. C. M.; GUEDES, L.; GUIMARÃES, P. M.; FÁVERO, A. P.; BERTIOLI, D. J. |
|
Título: |
Isolamento de seqüências de Arachis stenosperma em resposta à infecção de Puccinia arachidis e Cercosporidium personatum por cDNA-AFLP. |
|
Ano de publicação: |
2005 |
|
Fonte/Imprenta: |
Brasília: Embrapa Recursos Genéticos e Biotecnologia, 2005. |
|
Páginas: |
16 p. |
|
Série: |
(Embrapa Recursos Genéticos e Biotecnologia. Boletim de Pesquisa e Desenvolvimento, 94) |
|
Idioma: |
Português |
|
Palavras-Chave: |
Arachis stenosperma; cDNA-AFLP; Cercosporidium personatum; Ground nut; Sequeciamento. |
|
Thesagro: |
Amendoim; Fungo. |
|
Thesaurus NAL: |
fungi; Puccinia arachidis. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CENARGEN/26631/1/bp094.pdf
|
|
Marc: |
LEADER 00935nam a2200277 a 4500
001 1187041
005 2016-02-22
008 2005 bl uuuu u0uu1 u #d
100 1 $aLEAL-BERTIOLI, S. C. M.
245 $aIsolamento de seqüências de Arachis stenosperma em resposta à infecção de Puccinia arachidis e Cercosporidium personatum por cDNA-AFLP.$h[electronic resource]
260 $aBrasília: Embrapa Recursos Genéticos e Biotecnologia$c2005
300 $a16 p.
490 $a(Embrapa Recursos Genéticos e Biotecnologia. Boletim de Pesquisa e Desenvolvimento, 94)
650 $afungi
650 $aPuccinia arachidis
650 $aAmendoim
650 $aFungo
653 $aArachis stenosperma
653 $acDNA-AFLP
653 $aCercosporidium personatum
653 $aGround nut
653 $aSequeciamento
700 1 $aGUEDES, L.
700 1 $aGUIMARÃES, P. M.
700 1 $aFÁVERO, A. P.
700 1 $aBERTIOLI, D. J.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
