Data/hora: 08/06/2024 01:15:35

Biblioteca(s): | Embrapa Meio Norte / UEP-Parnaíba.
Data corrente: | 01/11/1995
Data da última atualização: | 01/11/1995
Autoria: | CHOI, P. S.; SOH, W. Y.; KIM, Y. S.; YOO, O. J.; LIU, J. R.
Título: | Genetic transformation and plant regeneration of watermelon using Agrobacterium tumefaciens.
Ano de publicação: | 1994
Fonte/Imprenta: | Plant Cell Reports, v.13, n.6, p.344-348, 1994.
Idioma: | Inglês
Conteúdo: | Adventitious shoots formed on the proximal cut edges of different cotyledonary explants of watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai; cvs. Sweet Gem and Gold Medal] cultured on Murashige and Skoog's (MS) medium with 1 mgl-1 6-benzyladenine (BA). Light (16-h photoperiod, about 7 Wm-2 cool-white fluorescent lamps) was essential for shoot formation. To obtain transformed plants, cotyledonary explants of "Sweet Gem" were cocultured with Agrobacterium tumefaciens LBA4404, a disarmed strain harboring a binary vector pBI121 carrying the CaMV 35S promoter-belta-glucuronidase (GUS) gene fusion used as a reporter gene and NOS promoter-neomycin phosphotransferase gene as a positive selection marker, for 48h on MS medium with 1 mgl-1 BA and 200 uM beta-hydroxyacetosyringone. After 48h of culture, explants were transferred to medium with 1 mgl-1 BA, 250 mgl-1 carbenicillin, and 100 mgl-1 kanamycin and cultured in the light. Adventitious shoots formed on the explants after 4 weeks of culture. When subjected to GUS histochemical assay, young leaves obtained from the shoots showed a positive response at a frequency of up to 16%. Preculturing cotyledonary explants on MS medium with 1 mgl-1 BA for 5d enhanced the competence of the cells to be transformed by Agrobacterium Southern blot analysis confirmed that the GUS gene was incorporated into the genomic DNA of the GUS-positive regenerants. The transformed plants were grown to maturity.
Palavras-Chave: | Melhoramento genetico; Watermelon.
Thesagro: | Agrobacterium Tumefaciens; Melancia.
Thesaurus Nal: | genetic transformation.
Categoria do assunto: | --
Registro original: | Embrapa Meio Norte / UEP-Parnaíba (CPAMN-UEPP)
Exemplares: | CPAMN-UEPP - (4370-1) - ADP - SP - -- -  -  - 1995.00158 - DIS